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  • 1
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A mesoscale model soil and ground-water system was constructed and instrumented to study physical, chemical, and microbial processes, including transport and degradation of contaminants. The model system was 4.6 m in height by 2.4 m in diameter and contained up to 65 tonnes of soil and geologic materials. The model was filled with Ap (11 cm), B (20 cm), C1 and C2 (369 cm) horizons; the water table was 3.3 m from the soil surface. A rain simulation system applied uniform coverage of rain and/or chloride tracer to the model. Sampling of soil, sediment, pore waters, and gas phase was carried out using a series of in situ collectors and sampling ports. Major anions, metals, pH, alkalinity, CO2, N2, O2, CH4, microbial numbers, microbial biomass and heterotrophic potential (14 C-amino-acid uptake) were monitored over time (120 days) and depth. The system approached steady state with respect to solution chemistry and gases after approximately 60–70 days. During this interval, carbon dioxide rose from atmospheric to 3–5% of total gas volume measured at each sampling depth above the water table. Physical, chemical, and biological conditions in the model system after the initial 120 days were comparable to those reported for vadose and saturated zones during field studies on shallow phreatic aquifers. These results indicated that the mesoscale model preserved many of the features of natural systems while providing controlled conditions for studies such as evaluating the fate, and ground-water contamination potential, of agricultural and industrial chemicals.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Various biotic and abiotic measurements were made over a 48-h period in a small lava-lakelet on the sub-Antarctic Marion Island. Typical oligotrophic conditions prevailed, with low biomass values and rates of productivity for the phytoplankton and bacteria. The bacteria and phytoplankton were subject to heavy zooplankton grazing pressures, which in turn were responsible for rapid carbon turnover rates in the lavalakelet. Diel cycles were observed in bacterial production and algal photosynthesis, with higher rates during the day time. No definite pattern could be seen for the zooplankton, either in terms of filtering rates or migratory activities. Phytoplankton productivity and zooplankton grazing and respiration were responsible for the greatest flow of carbon. Zooplankton occupied the top of the intermediate food chain and dominated the “live” organic carbon content of the water. A cycling index of 44% indicated a fairly closed system where most components were important in determining the overall structure of the system.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Scanning confocal laser microscopy was used to directly visualize accumulation of the herbicide diclofop methyl and its breakdown products by a degradative biofilm community, cultivated in continuous-flow cell cultures. Some bacterial cells accumulated these compounds. However, most accumulation occurred in cell capsules and certain regions of the exopolymer matrix. Mass spectroscopic analysis of the biofilm material confirmed accumulation of the parent compound and its breakdown products in the biofilms. Lower molecular weight degradation products were found in the effluent, indicating mineralization of diclofop by the flow cell cultures. Grazing protozoa feeding on the biofilms nonselectively ingested cell capsules and exopolymers, suggesting direct transfer and accumulation of the contaminants in protozoa. These findings demonstrated that microbial exopolymers can play an important role in the bioaccumulation of contaminants in natural systems.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5117
    Keywords: hypertrophic impoundment ; overturn ; oxygen ; nitrogen ; primary production ; mixing effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A major decrease in air temperature with an increase in wind stress created thermal instability resulting in overturn. Surface oxygen values decreased from 12.6 (170% saturation) prior to overturn to 0.4 mg · l−1 (5.7%) after overturn. Recovery of surface oxygen concentrations to supersaturation took approximately three months. Changes in the nitrogen balance after overturn indicated that extremely high rates of nitrification occurred. Little change in phosphorus concentration was observed. Surface chlorophyll concentrations decreased from 235 to approximately 2 mg · m−3, resulting in very low primary production values (Amax = 12.4 mg C m−3 h−1). Reduced rates of primary production were maintained for several months after overturn, mainly owing to the low ratio of Zeu/Zm (0.17). No significant changes occurred in the total bacterial population other than a redistribution within the water column. Significant changes occurred in the distribution and density of the zooplankton population. No fish kills were observed although fish populations were oxygen-stressed.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-3017
    Keywords: amphipods ; triallate ; lipids ; prairie ; autoradiography ; herbicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: A three-tiered approach involving autoradiography, measurement of seasonal lipid patterns and deployment of in situ microcosms, was used to assess the potential of the moderately lipophilic herbicide triallate to accumulate in two amphipod species (Hyalella azteca and Gammarus lacustris) in two prairie lakes and one prairie pond. Autoradiography revealed that the storage sites for triallate in amphipod tissues were associated with lipid-rich tissues, in particular, with triacylglycerol storage sites and the nervous system. Seasonal lipid patterns (total lipid) of amphipods did not differ amongst sites within a waterbody, however, there were marked differences amongst water bodies. Female amphipods had higher lipid contents than males during the reproductive period in early summer but this difference diminished later in the summer. Bioaccumulation factors of triallate in amphipod tissues ranged from 72 to 80 times the nominal water concentrations in 7 day microcosm trials. Triacylglycerol content and triallate body burden were positively correlated (r 2=0.58–0.91) for H. azteca in the two lakes in which the microcosms were deployed. However, no significant correlations were obtained between triacylglycerol content and triallate body burden for G. lacustris. Triacylglycerol contents in the amphipods were generally highest in spring and autumn, coinciding with application times for triallate.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 74 (1994), S. 103-120 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The effects of water infiltration into an alkaline coal combustion waste burial site on the chemical and microbiological aspects of a meso-scale (2.44 m diameter × 4.6 m, height, 65 tonne) model aquifer were analyzed. The spatial and temporal effects of the alkaline leachate on microbial activity, numbers and diversity were examined in the model and compared with uncontaminated control materials. Within the saturated zone below the waste there was a pH gradient from 12.4 at the water table, immediately below the waste, to 6.0 at 3.5 meters from the waste, and elevated levels of arsenic and strontium in the pore waters. Microtox testing of the contaminated pore waters indicated high toxicity (a gamma value of 1 at dilutions of 45 to 110 fold). The leachate contamination was associated with a reduction in bacterial [3H] leucine incorporation from a high of 267 fmol g−1 h−1 in sediments below the contaminant plume to undetectable in the contaminated zone. In comparison, leucine incorporation rates in control column sediments were 899 fmol g−1 h−1. Similar toxic effects were evident in reduced total direct and culturable counts of bacteria. Observations also indicated a reduction in microbial diversity and development of alkaline-tolerant microbial communities. These results indicated that any failure of confinement technologies at disposal sites would adversely affect both the chemistry and microbiology of the underlying saturated zone.
    Type of Medium: Electronic Resource
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