Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Permanent increase of immunocytochemical reactivity for γ-aminobutyric acid (GABA), glutamic acid decarboxylase, mitochondrial enzymes, and glial fibrillary acidic protein in rat cerebral cortex damaged by early postnatal hypoxia-ischemia (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 612-627 
    Details
    ISSN: 1432-0533
    Keywords: Hypoxia-ischemia ; Rat ; Perinatology ; Cerebral cortex ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A former study indicated that hypoxicischemic encephalopathy in rat sustained during early postnatal life may result in permanent epileptic activity in the baseline electroencephalogram. We, therefore, investigated whether the presumed higher firing frequency and metabolic activity of neurons in such hypoxia-damaged cortical areas would be reflected by an enhanced light microscopic immunoreactivity of γ-aminobutyric acid (GABA), the two isoforms of glutamic acid decarboxylase (GAD67 and GAD65), the mitochondrial enzymes cytochrome c oxidase and ATP synthase, and/or glial fibrillary acidic, protein (GFAP). To that end rat pups, 12–13 days of age, were unilaterally exposed to hypoxic-ischemic conditions and, after a survival period of 2 and 61/2 months, respectively, killed by perfusion fixation. After dissection of the brain, coronal vibratome sections of animals showing cortical damage were immunostained for the presence of the abovementioned antigens. Subsequent qualitative analysis revealed that the surroundings of cortical infarctions were unambiguously characterized by a disordered neural network containing numerous nerve cells, fibers and/or endings showing an enhanced immunoreactivity for GABA, both isoforms of glutamic acid decarboxylase, and cytochrome c oxidase and ATP synthase, while the astrocytes showed an enhanced immunoreactivity for GFAP. The diverse patterns of enhanced immunoreactivity suggested, furthermore, a wider low-to-high range of metabolic activities in both excitatory and inhibitory neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293323
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Evidence from Confocal Fluorescence Microscopy for a Dense, Reciprocal Innervation Between AVP-,somatostatin-, VIP/PHI-, GRP- and VIP/PHI/GRP-immunoreactive Neurons in the Rat Suprachiasmatic Nucleus (1997)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 9 (1997), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The rat suprachiasmatic nucleus (SCN) consists of several classes of neurons which can be identified by their transmitter content. Knowledge of putative interaction between these different cell types is essential in order to understand the possibilities of information processing within the SCN. The aim of the present study was therefore to obtain more information about the mutual innervation between the main cell classes in the rat SCN, viz. those containing the neuropeptides arginine vasopressin (AVP), vasoactive intestinal peptide (VIP), peptide histidine isoleucine (PHI), gastrin-releasing peptide (GRP) and somatostatin respectively. For this purpose, vibratome sections were double-immunolabelled for seven different peptide combinations and subsequently analysed by high-resolution confocal laser scanning fluorescence microscopy. Attention was focused on axosomatic appositions, the occurrence and frequency of which were quantitatively estimated. Our analysis of double-immunolabelled sections demonstrated that some of the VIP- and some of the GRP-immunoreactive nerve cells and endings showed colocalization. Assuming, on the basis of literature data, that VIP and PHI are always colocalized at the cellular level, the five main cell classes in the SCN appeared to be interconnected, at least axosomatically, in the following reciprocal way: AVP ↔ VIP/PHI, AVP ↔ GRP, AVP ↔ somatostatin, somatostatin ↔ VIP/PHI, somatostatin ↔ GRP, VIP/PHI ↔ GRP, VIP/PHI/GRP ↔ GRP, VIP/PHI/GRP ↔ VIP/ PHI. In addition to this heterologous axosomatic innervation, these cell groups also showed substantial homologous innervation. Supported by electron microscope data from the literature showing the existence of axodendritic synapses for some of these peptide combinations, our findings strongly suggest that the rat SCN comprises a complex synaptic network with strong interactive capabilities, which is probably a requisite for its biological clock function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.1997.tb01691.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Diurnal variations in number of Golgi-dense core vesicles in light pinealocytes of the rabbit (1976)
    Springer
    Journal of neural transmission 38 (1976), S. 231-237 
    Details
    ISSN: 1435-1463
    Keywords: Pineal organ ; rabbit ; ultrastructure ; diurnal rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Evidence is given of diurnal rhythms in the mean number of dense-core vesicles surrounding a Golgi complex in the perikaryon of light rabbit pinealocytes and of those present per terminal of light pinealocyte processes. These rhythms show their top at noon and in the evening, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01249440
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Electron microscopic evidence of glycogen storage in the dark pinealocytes of the rabbit pineal gland (1977)
    Springer
    Journal of neural transmission 40 (1977), S. 69-79 
    Details
    ISSN: 1435-1463
    Keywords: Pineal ultrastructure ; rabbit ; pigment ; glycogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Electron microscopic evidence is given that the small dark granules in the rabbit dark pinealocytes represent glycogen. The conception is put forward that this glycogen, synthesized at the membranes of the rough endoplasmic reticulum, is taken up by lysosomes to participate then in the conversion of the lysosomes into pigment bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01250281
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    The ultrastructure of the rabbit pineal gland after sympathectomy, parasympathectomy, continuous illumination, and continuous darkness (1975)
    Springer
    Journal of neural transmission 36 (1975), S. 183-194 
    Details
    ISSN: 1435-1463
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the rabbit pineal gland was investigated after sympathectomy (extirpation or decentralization of the superior cervical ganglia), parasympathectomy, continuous illumination and continuous darkness. The similarity of the ultrastructural changes in the light pinealocytes occurring after sympathectomy and after continuous illumination was striking. It is supposed that these changes have a common cause,viz. the lack of free noradrenaline, the pinealotropic neurotransmitter. The smooth endoplasmic reticulum present in the terminals of the offshoots of the light pinealocytes is possibly involved in pineal indoleamine synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253125
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Structure and innervation of the pineal gland of the rabbit,Oryctolagus cuniculus (L.) (1973)
    Springer
    Cell & tissue research 139 (1973), S. 473-485 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rabbit ; Architecture ; Autonomic innervation ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A light microscopic investigation of the rabbit pineal gland with the aid of silver-stained sections gave the following results. In the gland a medulla and a cortex can be distinguished, the medulla containing so-called light and dark pinealocytes, the cortex only light ones. Autonomic nerve fibres reach the pineal organ by two routes: (1) via the perivascular spaces of pineal blood vessels and (2) via two distinct nerve bundles, the nervi conarii. Bilateral superior cervical ganglionectomy revealed that these pinealo-petal nerve fibres are mainly orthosympathetic postganglionic. Intramural pineal neurones with synaptic-like structures on their cell bodies and dendrites point to the presence of a parasympathetic innervation next to the orthosympathetic one. Direct afferent or efferent neural connections with the brain appeared to be absent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02028389
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Electron microscopy of the rabbit pineal organ in vitro (1976)
    Springer
    Cell & tissue research 172 (1976), S. 365-377 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Rabbit ; Organ culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The results presented in this study show that the rabbit pineal organ cultured in vitro retained its in vivo fine structure for at least eight days. However, the Golgi complex in the light pinealocytes stopped forming dense core vesicles while vesicle-crowned ribbons increased in number. After addition of norepinephrine to the culture medium, the Golgi complex once more began the production of dense core vesicles. Terminals of light pinealocytic processes then often contained Golgi dense core vesicles in close contact with the cell membrane, suggesting the release of the vesicular content into the intercellular and perivascular spaces. A close topographical relationship between Golgi dense core vesicles and vesicle-crowned ribbons was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399519
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    A pharmacological and autoradiographic study on the ultrastructural localization of indoleamine synthesis in the rabbit pineal gland (1977)
    Springer
    Cell & tissue research 185 (1977), S. 199-214 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland, rabbit ; Organ culture ; Ultrastructure ; Indoleamine synthesis ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to localize pineal indoleamine synthesis at the ultrastructural level, an electron microscopic analysis was carried out on rabbit pineal tissue, cultured for several days in a medium containing the inhibitor p-chlorophenylalanine. Furthermore, electron microscopic autoradiography was applied to rabbit pineal tissue cultured in a medium containing the tritium labeled precursor 5-hydroxytryptophan. p-Chlorophenylalanine altered the ultrastructure of the mitochondria in the light pinealocytes only. Incubation with 3H-5-hydroxytryptophan caused a moderate and uniform labeling of the pinealocytes. These data suggest that the synthesis and storage of indoleamines in the rabbit pineal gland do not take place within the conventional membrane-limited cell organelles but more diffusely in the cytosol of the light pinealocytes. The spherical enlargement of the mitochondria in the light pinealocytes after culturing with p-chlorophenylalanine supports the biochemical findings of Hori et al. (1976) that the mitochondria are the carriers of the enzyme tryptophan 5-hydroxylase. The subsequent decarboxylation of 5-hydroxytryptophan to serotonin probably occurs free in the cytosol. The strong labeling of the noradrenergic nerve endings (in contrast to the cholinergic ones) after incubation with 3H-5-hydroxytryptophan demonstrates that the noradrenergic nerve endings in the rabbit pineal gland can, at least in vitro, selectively take up 5-hydroxytryptophan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220665
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Structure and innervation of the pineal gland of the rabbit, Oryctolagus cuniculus (L.) (1975)
    Springer
    Cell & tissue research 157 (1975), S. 25-51 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland (Rabbit) ; Autonomic innervation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the rabbit pineal gland two types of postganglionic nerve endings were found which are characterized by the presence of small dense-core vesicles or small clear vesicles. Pharmacological and cytochemical experiments showed them to be noradrenergic and cholinergic, respectively. Both types were often present in the same nerve bundle, occasionally in close apposition. Intrapineal neurons were only rarely observed. They showed cholinergic synapses on their perikaryon and dendrites as well as noradrenergic axo-dendritic close eontacts. Bilateral extirpation of the superior cervical ganglia revealed the postganglionic sympathetic origin of the pineal noradrenergic nerve fibres. Moreover, it appeared that these ganglia are hardly, if at all, involved in the pathway of pineal cholinergic innervation. The results obtained from lesions of both facial nerves, taken together with the results reported in the literature, led to the conclusion that the postganglionic cholinergic nerve fibres in the pineal are of parasympathetic origin. A model for the sympathetic and parasympathetic pineal innervation is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223229
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Structure and innervation of the pineal gland of the rabbit, oryctolagus cuniculus (L.) (1973)
    Springer
    Cell & tissue research 141 (1973), S. 545-560 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland (Rabbit) ; Light and dark pinealocytes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the rabbit pineal gland a cortex and a medulla can be distinguished, based on the distribution pattern of two cell types. The medulla consists of so-called light and dark pinealocytes, whereas in the cortex only light ones occur. The cytological distinction between the two cell types is principally due to the exclusive presence of abundant pigment granules in the dark pinealocytes. The light pinealocytes have long offshoots. Their club-shaped terminals are crowded with smooth endoplasmic reticulum from which grey vesicles are pinched off. In the medulla, these offshoots run for the most part into the direction of blood vessels to terminate in close topographical relationship with dark pinealocytes which are mainly arranged around these vessels. The ultrastructure of both the light and the dark pinealocytes is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307124
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...