ISSN:
1432-0428
Keywords:
Diabetes mellitus
;
proteinuria
;
nephropathy
;
SDS-PAGE
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Renal damage is one of the most serious complications of diabetes mellitus. Most methods used to detect kidney malfunction show abnormalities only in the advanced stages. To find whether sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the urinary proteins could give earlier indications of kidney malfunction, 68 patients with maturity-type diabetes and 22 with juvenile-type diabetes have been studied. Quantitative determination of proteinuria, urinary lysozyme, creatinine clearance and SDS-PAGE of urinary proteins were performed. Diabetics of both types with disease of more than 10 years duration showed significantly greater proteinuria and lysozymuria, higher serum creatinine values and lower creatinine clearances than their respective controls. Such differences were not seen in patients with diabetes of short duration. SDS-PAGE allowed detection of a higher proportion of patients with abnormalities. Thus, 3 out of 10 patients with juvenile diabetes of short duration showed predominant excretion of low molecular weight proteins, suggesting tubular dysfunction. This was not observed in the other groups of diabetics where increased elimination of high molecular weight proteins was noted, suggesting glomerular damage. SDS-PAGE revealed a higher frequency of abnormalities than other tests of renal function, with 67% abnormal in juvenile-type diabetes of greater than 10 years duration, 45% in maturity-type diabetes of less than 10 years duration and 76% abnormal in maturity-type diabetes of greater than 10 years duration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01219793
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