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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of a mutant cell line derived from ICR 2A frog cells hypersensitive to the induction of nondimer DNA damage by solar ultraviolet radiation (1985)
    Springer
    Somatic cell and molecular genetics 11 (1985), S. 339-344 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A mutant cell line DRP 36, hypersensitive to nondimer DNA damage induced by exposure of cells to the Mylar-filtered solar ultraviolet (UV) radiation produced by a fluorescent sunlamp plus photoreactivating light (PRL) was isolated from the haploid ICR 2A frog cell line. The D0 for mutant cells exposed to this solar UV source was 3.3 kJ/m2 compared with a D0 of 7.3 kJ/m2 for the parental ICR 2A cells. In contrast, DRP 36 and ICR 2A cells exhibited similar levels of survival following 254-nm irradiation which causes the induction primarily of pyrimidine dimers. The cross-sensitivity to additional DNA damaging agents was examined, and it was determined that the DRP 36 cells are also hypersensitive to treatment with γ-rays, ethyl methane sulfonate (EMS), cis-dichlorodiammine platinum (II) (DDP), and 4-nitroquinoline oxide (4-NQO) while exhibiting normal sensitivity tol-phenylalanine mustard (L-PAM), 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) and mitomycin C (MMC).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01534410
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transfer of genetic information via isolated amphibian metaphase chromosomes (1978)
    Springer
    Somatic cell and molecular genetics 4 (1978), S. 341-353 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The metaphase chromosome transfer system of McBride and Ozer (1973) has been adapted to a haploid, euploid, frog cell line. Genes coding for a deoxypyrimidine kinase and an enzyme responsible for a thymidine-specific saturable transport system have each been transferred at frequencies between 10−6 and 10−5 transferents per cell treated. Revenants for each of these two genes were observed at frequencies between 10−8 and 10−7 revenants per cell tested. Selfing controls showed no transferents. Two colonies were obtained in which cotransfer of both genes may have occurred. Activities of the transferred genes were assayed by incorporation of [3H]thymidine into alkali-stable, acid-precipitable material. Growth properties of 13 transferents in various media were also determined and presence of the appropriate enzymes inferred. These transferents were tested for stability early (25 generations) after transfer and were found to be stable. All 13 transferents possess the normal haploid number of chromosomes (n=13) with no cytologically detectable chromosomal fragments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01542847
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning of the human geneSUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation (1995)
    Springer
    Somatic cell and molecular genetics 21 (1995), S. 255-263 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring a normal DPC response and solar UV sensitivity to DRP 153. This gene has been designatedSUVCC3 to denote solar UV cross-complementing gene number 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02255780
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    ATM heterozygosity and breast cancer: screening of 37 breast cancer patients for ATM mutations using a non-isotopic RNase cleavage-based assay (2000)
    Springer
    Breast cancer research and treatment 61 (2000), S. 79-85 
    Details
    ISSN: 1573-7217
    Keywords: ataxia telangiectasia ; ATM ; breast cancer ; mutation screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Based upon the results of several epidemiologic studies, it has been suggested that women who are carriers for a mutation in the ataxia telangiectasia-mutated (ATM) gene are susceptible for the development of breast cancer. Therefore, 37 consecutive breast cancer patients were screened for the presence of a germline ATM mutation using a non-isotopic RNase cleavage-based assay (NIRCA). This paper reports the first use of NIRCA for detection of ATM mutations in breast cancer patients. Using this assay, no ATM mutations were found in our patient population. This result is similar to the findings of other studies that have employed approaches complementary to NIRCA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006463730337
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    Paper (German National Licenses)
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