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  • 1
    Electronic Resource
    Electronic Resource
    Lipoxin Biosynthesis by Trout Macrophages Involves the Formation of Epoxide Intermediates (1994)
    s.l. : American Chemical Society
    Biochemistry 33 (1994), S. 856-863 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00170a002
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth Galleria mellonella in vitro (1979)
    Springer
    Cell & tissue research 199 (1979), S. 127-137 
    Details
    ISSN: 1432-0878
    Keywords: Haemocytes ; Phagocytosis ; Lysosomes ; Acid phosphatase ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The plasmatocytes are the major phagocytic blood-cell type in the haemolymph of the wax-moth, Galleria mellonella. In the present study, these cells were allowed to attach to tissue culture dishes for 1 h, rinsed and then incubated with latex beads for up to 72 h. These cells were then fixed for routine transmission electron microscopy and acid phosphatase cytochemistry. Intracellular latex particles were found in tight, ill-defined phagosomes, which were often clearly associated with the Golgi complexes of the plasmatocytes. Fusion of both primary lysosomes and multivesicular bodies with the phagosomes occasionally occurred and this resulted in the accumulation of an acid phosphatase positive reaction product around the test particles. Subsequent experiments showed that this acid phosphatase activity was mainly associated with the primary lysosomes. The results of the lysosome/latex interactions are compared with those obtained from similar studies on the digestive mechanisms in other phagocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237732
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The role of the haemocytes of Clitumnus extradentatus in haemolymph coagulation (1977)
    Springer
    Cell & tissue research 182 (1977), S. 513-524 
    Details
    ISSN: 1432-0878
    Keywords: Haemocytes ; Insects ; Haemolymph coagulation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron-microscopic observations of the blood cells (haemocytes) of the stick insect Clitumnus extradentatus in vitro showed that two morphologically distinct cell types, the cystocytes and granular cells are involved in haemolymph coagulation. Both these cell types contain a variable number of electron-dense granules which upon release cause progressive coagulation and precipitation of the haemolymph. In the cystocytes this release is extremely rapid (30–60s) while in the granular cells it only occurs after 1–2min in vitro. The role of these cells in haemostasis is discussed together with the possible significance of the involvement of two cell types in the coagulation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219833
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural, cytochemical and functional studies on the eosinophilic granulocytes of larval lampreys (1985)
    Springer
    Cell & tissue research 240 (1985), S. 705-709 
    Details
    ISSN: 1432-0878
    Keywords: Eosinophilic granulocytes ; Phagocytosis ; Cytochemistry ; Comparative haematology ; Acid phosphatase ; Lampreys, ammocoetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Blood from larval lampreys (ammocoetes) contains a small number of eosinophilic granulocytes which are formed in the protospleen and kidney. Both immature and mature forms of this cell type are present in the blood and these are easily identified from other cell types due to the prominent eosinophilic granules that fill the cytoplasm. Ultrastructurally, these granules are electron-dense, largely unstructured, Golgi-derived and contain acid phosphatase but not peroxidase. Eosinophilic granulocytes ingest bacteria but fail to internalise colloidal carbon. The functional and phylogenetic significance of these cells is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216359
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructural and cytochemical studies on the blood cells of the sea squirt,Ciona intestinalis (1982)
    Springer
    Cell & tissue research 223 (1982), S. 403-414 
    Details
    ISSN: 1432-0878
    Keywords: Tunicates ; Stem cells ; Amoebocytes ; Acid phosphatase ; Peroxidase ; Comparative haematology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure and cytochemistry of the stem cell and amoebocytic blood cells of the sea squirt,Ciona intestinalis, are described. The stem cells are characterised by a high nuclear: cytoplasmic ratio and an undifferentiated cytoplasm. In thin sections, four types of amoebocytes can be demonstrated: non-vacuolar and vacuolar hyaline amoebocytes, granular amoebocytes, and refractile amoebocytes. The cytoplasm of non-vacuolar amoebocytes contains many vesicles, which although structurally similar to primary lysosomes are in most cases acid phosphatase-negative. The granular amoebocytes enclose several microtubule-containing granules and vacuoles with electron-dense vanadium deposits, while the refractile amoebocytes are characterised by their pleomorphic inclusions and spike-like pseudopodia. Both the granules and inclusions of these amoebocyte types are peroxidaseand acid phosphatase-negative indicating that they are probably not lysosomes. The possible interrelationships of the four amoebocyte types are discussed, and it is suggested that the vacuolar hyaline amoebocytes may differentiate into granular amoebocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01258497
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  • 6
    Electronic Resource
    Electronic Resource
    A histological study of wound healing and hemocyte function in the wax-moth Galleria mellonella (1978)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 157 (1978), S. 181-199 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The stages in wound healing of the wax-moth, Galleria mellonella, were examined using routine histological techniques. Final instar larvae were wounded and maintained for up to 72 hours before fixation, dehydration and embedding in Paraplast wax or Araldite.Immediately after wounding both fat-body and hemolymph were forced through the wound to form a plug. Concomitantly, the hemolymph coagulated forming vast networks of strand-like material attached to the hemocytes underlying the wound. After one to two hours this plug melanized and the cells became highly necrotic. Six hours after wounding, there was a massive influx of hemocytes which eventually attached to the melanized layer over the wound to form a multicellular sheath. Twelve to twenty-four hours later, the epidermal cells underlying the broken cuticle detached and migrated across the wound to form a new intact layer. This layer secreted an amorphous material which is probably the precursor of the new cuticle. Hemocyte functions during wound healing and other cellular defenses are discussed.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051570206
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    Paper (German National Licenses)
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