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  • 1
    ISSN: 1432-072X
    Keywords: Ribulose bisphosphate carboxylase/oxygenase (RuBisCO) ; pBR322-transformants ; Anacystis nidulans ; Growth ; RuBisCO amplification ; Plasmid-chromosomal recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Prior research suggested that the genes for large (L) and small (S) subunits of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) are amplified in ampicillin-resistant pBR322-transformants of Anacystis nidulans 6301. We now report that chromosomal DNA from either untransformed or transformed A. nidulans cells hybridizes with nick-translated [32P]-pBR322 at moderately high stringency. Moreover, nick-translated [32-P]-pCS75, which is a pUC9 derivative containing a PstI insert with L and S subunit genes (for RuBisCO) from A. nidulans, hybridizes at very high stringency with restriction fragments from chromosomal DNA of untransformed and transformed cells as does the 32P-labeled PstI fragment itself. The hybridization patterns suggest the creation of two EcoRI sites in the transformant chromosome by recombination. In pBR322-transformants the RuBisCO activity is elevated 6- to 12-fold in comparison with that of untransformed cells. In spite of the difference in RuBisCO activity, pBR322-transformants grow in the presence of ampicillin at a similar initial rate to that for wild-type cells. Growth characteristics and RuBisCO content during culture in the presence or absence of ampicillin suggest that pBR322-transformants of A. nidulans 6301 are stable. The data also collectively suggest that a given plasmid in the transformed population replicates via a pathway involving recombination between the plasmid and the chromosome.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 47 (1982), S. 151-153 
    ISSN: 1432-1106
    Keywords: Sciatic nerve ; Excitability ; Field potential effects ; Ephaptic interaction ; Intraaxonal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intra-axonal recordings were obtained in vitro from frog sciatic nerve axons. Adjusting whole nerve stimulation current to just subthreshold for the impaled axon elicited triphasic threshold changes in that axon. Threshold changes were determined by direct intra-axonal current application. Graded subthreshold depolarizations were present in many axons during the passage of action potentials in surrounding axons. When nerve branches were stimulated and axon recordings were obtained from the main nerve trunk, both branches, the one that activated the impaled axon and the one that did not, elicited threshold changes in the impaled axon. These data indicate on a cellular level that impulse activity in an intact nerve bundle can modulate the excitability of adjacent nonactivated fibers.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 267-279 
    ISSN: 0392-6737
    Keywords: Elastic scattering of electrons by atoms and molecules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The many-body correlation forces which act between the impinging electron and the bound electrons of the target atoms are treated here using a newly developed correlation-polarisation potential that originates from the calculation of correlation energies in electronic bound states of atoms and molecules. The new formulation of such forces is shown to be particularly effective for the present systems once all the other forces at play are treated exactly within the Hartree-Fock scheme. The calculations of this paper compare our new density functional theory (DFT) approach (Gianturco and Rodriguez-Ruiz, 1993) with an earlier homogeneous electron gas (FEG) model for correlation forces (O'Connell and Lane, 1983) and clearly indicate the superior quality of a more realistic Hartree-Fock description of the density functional for the bound electrons. Excellent accord is obtained between experimental cross-sections and theoretical results, both for differential and integral elastic electron scattering cross-sections, over a very broad range of collision energies.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Environment and Resources 23 (1998), S. 499-536 
    ISSN: 1056-3466
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The environment has emerged as an important determinant of the performance of the modern chemical industry. This paper reviews approaches for incorporating environmental issues into the design of new processes and manufacturing facilities. The organizational framework is the design process itself, which includes framing the problem and generating, analyzing, and evaluating alternatives. A historical perspective on the chemical process synthesis problem illustrates how both performance objectives and the context of the design have evolved to the point where environmental issues must be considered throughout the production chain. In particular, the review illustrates the need to view environmental issues as part of the design objectives rather than as constraints on operations. A concluding section identifies gaps in the literature and opportunities for additional research.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1434-601X
    Keywords: 25.70 Gh ; 24.30 Cz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Continuumγ- ray spectra from the decay of59Cu formed at an excitation energy of 100 MeV and angular momenta up to 43ħ by means of the reaction 190 MeV32S +27Al have been measured and analyzed. The parameters of the Giant Dipole Resonance (GDR) have been extracted using the statistical model. The derived GDR width confirms the sizeable broadening of this resonance in59Cu already reported in our earlier investigation at 77 MeV excitation energy (Jcrit=38ħ). Estimates of the GDR width have been performed in the adiabatic approximation. Predicted values account qualitatively for the experimental data of59Cu as well as of the heavier isotope63Cu, in which the broadening was not seen up to 77MeV excitation (Jcrit=35ħ). The present analysis demonstrates the strong sensitivity of the GDR to spin effects in this mass region.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1434-601X
    Keywords: 25.70.Bc ; 25.70.Hi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Elastic and inelastic scattering as well as transfer transitions involving a valence proton in thesd- andfp- shell orbits are studied in the interaction of37Cl +36S at ECM=50 MeV. Experimental angular distributions of single particle states of37Cl (elastic and inelastic transfer) are presented with a CRC analysis. In the CRC calculations the effects of inelastic and transfer couplings are studied using known spectroscopic information. In the CRC analysis six single particle bound states and the collective 2+ excitation of36S are included in the coupling scheme. Higher order coupling effects are found to be important. A distinct effect, the mixing of single particle states (of different parity) due to direct and transfer interactions is observed to produce an enhancement of the transfer cross section to the low lying states. This feature is due to a change of the asymptotically defined single nucleon orbits via polarization in the field of the other nucleus, an effect which is analogous to hybridization, known from atomic physics.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Hyperfine interactions 89 (1994), S. 281-298 
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A rather simple semiclassical model is described for treating short-range correlation forces in the scattering of positrons from atoms at the energies below the thresholds of positronium formation in each of the considered systems. The present model allows one to introduce a non-empirical procedure for devising several damping functions, one for each of the perturbation terms which are included in the asymptotic expansion of the polarisation potential. It is shown by calculations, carried out for He, Ne and Ar as examples, that one can obtain very good agreement with experimental findings for surprisingly little computational effort, even for fairly complex atomic targets which would be otherwise out of reach for all but the most computationally intensive ab initio methods.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 31 (1994), S. 149-159 
    ISSN: 1434-6079
    Keywords: 34.80.Bm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The many-body correlation forces, which act between the impinging electron and the bound electrons of the two heaviest rare gas atoms, are treated here using a newly developed correlation-polarisation potential that originates from the calculation of correlation energies in electronic bound-states of atoms and molecules. The new formulation of such forces, already tested by us for the lighter atomic targets, is particularly effective for the present systems and can be implemented very easily even for heavy atomic targets. The calculations reported in this paper show clearly that very good accord is obtained with more sophisticated theoretical treatments and with several experimental data on integral cross sections, momentum transfer cross sections and angular distributions.
    Type of Medium: Electronic Resource
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