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  • 1
    Electronic Resource
    Electronic Resource
    Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 28 (2005), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non-growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 µmol m−2 s−1) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light-induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light-induced K+ flux changes are likely to play a charge balancing role. Light-stimulated Ca2+ influx was not directly attributable either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light-induced ion flux changes are associated with both leaf growth and photosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.2005.01270.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ionic and osmotic components of salt stress specifically modulate net ion fluxes from bean leaf mesophyll (2000)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 23 (2000), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ionic mechanisms of salt stress perception were investigated by non-invasive measurements of net H+, K+, Ca2+, Na+, and Cl− fluxes from leaf mesophyll of broad bean (Vicia faba L.) plants using vibrating ion-selective microelectrodes (the MIFE technique). Treatment with 90 m M NaCl led to a significant increase in the net K+ efflux and enhanced activity of the plasma membrane H+-pump. Both these events were effectively prevented by high (10 m M) Ca2+ concentrations in the bath. At the same time, no significant difference in the net Na+ flux has been found between low- and high-calcium treatments. It is likely that plasma membrane K+ and H+ transporters, but not the VIC channels, play the key role in the amelioration of negative salt effects by Ca2+ in the bean mesophyll. Experiments with isotonic mannitol application showed that cell ionic responses to hyperosmotic treatment are highly stress-specific. The most striking difference in response was shown by K+ fluxes, which varied from an increased net K+ efflux (NaCl treatment) to a net K+ influx (mannitol treatment). It is concluded that different ionic mechanisms are involved in the perception of the ‘ionic’ and ‘osmotic’ components of salt stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2000.00606.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    H+ flux kinetics around plant roots after short-term exposure to low temperature: identifying critical temperatures for plant chilling tolerance (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 20 (1997), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: H+ flux kinetics were measured in solution around the roots of chilling-tolerant pea (Pisum sativum) and bean (Vicia faba), chilling-sensitive cucumber (Cucumis sativus) and pumpkin (Cucurbita pepo), and intermediate corn (Zea mays) species using a microelectrode technique to measure net flux. As a root warmed to room temperature alter 90 min at 4°C, at which temperature the H+ flux was near zero, the flux rose (influx) and then fell. These changes occurred at two apparent critical temperatures, which were higher for the more chilling-sensitive species. The First, lower, apparent critical temperature may represent the start of passive inward H+ transport. The higher critical temperature may represent the start of active H+ extrusion. From these apparent critical temperatures we have calculated the real critical temperature and the time delay of the chilling signal transduction process. Passive and active H+ transporters appear to have the same real critical temperature of chilling sensitivity, about 9°C, but have, respectively, 4 min and 11 min time delays. Measurement of these apparent critical temperatures may provide quick and reliable screening for chilling sensitivity in plant breeding programmes. Future ion flux studies may show the cellular location of chilling stress perception and the signal transduction pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.1997.d01-35.x
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  • 4
    Electronic Resource
    Electronic Resource
    Electrophysiological localization of blue light sensory sites in etiolated dicotyledon seedlings (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 26 (2003), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Changes induced by blue light (BL) in ion fluxes and pH in etiolated seedlings of dicotyledons (Cucumis sativus L., Phaseolus lunatus L., Pisum sativum L.) were studied using a non-invasive ion-selective microelectrode technique (MIFE). BL induced significant Ca2+ uptake within the first 5 min after onset in upper parts of the whole cucumber seedling. To locate the sites of the BL perception, studies on excised segments were performed. The data obtained demonstrated that the observed Ca2+ uptake is associated with the very top of the hypocotyl hook of cucumber and butter bean, and with the whole epicotyl of pea, although the response was stronger for the upper parts of the epicotyl. Addition of Gd3+ and La3+ into the bathing solution completely prevented Ca2+ influx, implicating the involvement of Ca2+ channels in the regulation of Ca2+ transport during BL exposure. BL-induced changes in H+ and Ca2+ fluxes were not likely to be linked to the one mechanism, as they were separated spatially and pharmacologically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2003.01072.x
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  • 5
    Electronic Resource
    Electronic Resource
    Screening plants for salt tolerance by measuring K+ flux: a case study for barley (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 28 (2005), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Development of salt-tolerant genotypes is central both to remediation of salinity-affected land and to meet increasing global food demand, which has been driving expansion of cropping into marginal areas. The bottleneck of any breeding programme is the lack of a reliable screening technique. This study tested the hypothesis that the ability of plants to retain K+ under saline conditions is central to their salt tolerance. Using seven barley cultivars contrasting in salt tolerance (CM72, Numar, ZUG293, ZUG95, Franklin, Gairdner, ZUG403), a comprehensive study was undertaken of whole-plant (growth rate, biomass, net CO2 assimilation, chlorophyll fluorescence, root and leaf elemental and water content) and cellular (net fluxes of H+, K+, Na+ and Ca2+) responses to various concentrations of NaCl (20–320 m m). Na+ selective microelectrodes were found to be unsuitable for screening purposes because of non-ideal selectivity of the commercially available Na+ LIX. At the same time, our results show very strong negative correlation between the magnitude of K+ efflux from the root and salt tolerance of a particular cultivar. K+ efflux from the mature root zone of intact 3-day-old seedlings following 40 min pretreatment with 80 m m NaCl was found to be a reliable screening indicator for salinity tolerance in barley. As a faster and more cost-effective alternative to microelectrode measurements, a procedure was developed enabling rapid screening of large numbers of seedlings, based on amount of K+ leaked from plant roots after exposure to NaCl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.2005.01364.x
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