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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 83 (1997), S. 252-256 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Confocal laser scanning microscopy and transmission electron microscopy were used to study the inner membrane complex of tachyzoites of Toxoplasma gondii. DiOC6, a lipophilic cationic fluorescent dye used to visualize the endoplasmic reticulum of eukaryotic cells, labeled cytoplasmic structures in a reticulated pattern and the periphery of the nucleus of the host cell. Intracellular and extracellular tachyzoites were stained. Observation of several focal planes showed labeling of the most peripheral region of the protozoan. Reaction product was observed in the outer nuclear membrane, in profiles of the endoplasmic reticulum, and in the inner membrane complex of tachyzoites subjected to the KI-OsO4 technique. Taken together, these observations suggest that the inner membrane complex may represent a specialized region of the endoplasmic reticulum of tachyzoites of T. gondii.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 33 (1986), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . The fine structure of the paraxial rod of Phytomonas davidi and Herpetomonas megaseliae was analyzed in thin sections of Triton X-100-extracted, tannic acid-glutaraldehyde-fixed cells and in replicas of quick-frozen, freeze-fractured, deeply etched and rotary shadowed cells. The paraxial rod is formed by a complex array of filaments. Two regions, designated as proximal and distal, are formed by two and at least 11 plates, respectively, composed of an association of 25-nm and 7.0-nm-thick filaments which are oriented at an angle of-50° in relation to the major axis of the axoneme. The intermediate region is less dense and is formed by thin filaments. Short single and Y-shaped filaments connect the proximal plate to doublets numbers 4 and 7 of the axoneme. Based on the images obtained in stereopairs as well as in photographs obtained before and after inclination of the specimen, a three-dimensional model of the paraxial rod is proposed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 35 (1988), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The fine structure of trypanosomatids from the plants Euphorbia hyssopifolia, E. characias, E. pinea, and Manihot esculenta, cultivated under axenic conditions, was compared. All parasites had a structural organization characteristic of members of the Trypanosomatidae. The organization of the membranes of the endoplasmic reticulum (ER) varied according to the isolate. In Phytomonas francai (isolated from M. esculenta), the ER cisternae form parallel rows. In Phytomonas sp. from E. characias, a para-crystalline array of the ribosomes attached to the ER membranes was observed. The ER membranes found in Phytomonas sp. from E. pinea seemed to originate from the central portion of the protozoon, branching in all directions. The peroxisome-like organelle (glycosome) found in Phytomonas sp. from E. hyssopifolia and E. characias occasionally showed an organized array. Taken together, the morphological observations make it possible to distinguish the four isolates of Phytomonas.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Wachstein and Meisel incubation medium was used to detect ATPase activity in epimastigote, spheromastigote (amastigote), and bloodstream trypomastigote forms of Trypanosoma cruzi. Reaction product, indicative of enzyme activity, was associated with the plasma membrane covering the cell body and the flagellum of the parasite. No reaction product was found in the portion of the plasma membrane lining the flagellar pocket. The plasma membrane-associated ATPase activity was not inhibited by ouabain or oligomycin, was detected in incubation medium without K+, was inhibited by prolonged glutaraldehyde fixation, and its activity was diminished when Mg2+ was omitted from the incubation medium. The Ernst medium was used to detect Na+-K+-ATPase activity in T. cruzi. No reaction product indicative of the presence of this enzyme was detected. Reaction product indicative of 5'-nucleotidase was not detected in T. cruzi. Acid phosphatase activity was detected in lysosomes. These results indicate that a Mg2+-activated ATPase is present in the plasma membrane of T. cruzi and that it can be used as an enzyme marker, provided that the mitochondrial and flagellar ATPases are inhibited, to assess the purity of plasma membrane fractions isolated from this parasite.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The digestive system of the capybara has been investigated because of its coprofagia habits, important for their absorptive activity. These species present differences in terms of gastrointestinal morphological characters when compared with other rodents. Macroscopiclly, the stomach of the capybara is constituted of the following parts: cardiac, pyloric, body, fundic and gastric diverticulum. It presents two curvatures, one big and another small. Externally, the presence of gastric bands (tenias) is observed. With regards to the volumetric view, the gastric capacity varies from 850 to 2010 ml, with an average of 1498.57 ml. So, the stomach of this animal can be classified as a simple stomach, in the format of a curved sack and similar to an inverted letter ‘J’. The gastric mucous membrane presents a surface filled by numerous tortuous gastric folds and longitudinally distributed along all its extension. The mucous tunic also possesses recesses located among the successive gastric folds, which were denoted as gastric parts with numerous openings described as gastric pits. In the cardiac part, a glandular epithelium with cardiac glands is noticed containing a lot of parietal and mucous neck cells. The fundic part, body and gastric diverticulum contain proper gastric glands with main, parietal and mucous neck cells. Finally, the pyloric part has pyloric glands with two cellular types, mucous neck and parietal cells.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 20 (1973), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Trypanosoma cruzi has been cultured in pigment epithelial cells of the iris from the chick embryo. Melanin granules, identical with those of the host cells were found in the intracellular, amastigote (leishmania) forms. In many of the intracellular forms cytostome-like structures were seen, often in intimate contact with the pigment granules of the host cells, which suggests the uptake of melanin granules through the cytostomes by the process of intracellular phagotrophy.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 21 (1974), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The fine structure of the dividing nucleus in the intracellular amastigote forms of Trypanosoma cruzi from tissue cultures has been described. In the first phase of division the nucleus shows a homogenous structure owing to the dispersion of its chromatin and nucleolar material. Microtubules similar to those of a mitotic spindle in metozoan cells then appear, running from one pole to the other. They disappear when the division of the nucleus is complete and the chromatin and the nucleolar material reorganize into their former positions.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 814 (1985), S. 163-169 
    ISSN: 0005-2736
    Keywords: (T. cruzi) ; Cell surface ; Electrophoretic mobility ; Sialic acid ; Surface charge
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Journal of Biochemistry 20 (1988), S. 329-335 
    ISSN: 0020-711X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Journal for Parasitology 24 (1994), S. 899-907 
    ISSN: 0020-7519
    Keywords: Brugia malayi ; cuticle ; cytochemistry ; epicuticle ; freeze-fracture ; micronlariae ; surface ; ultrastructure
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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