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  • 1
    Digitale Medien
    Digitale Medien
    Absence of an effect of lead acetate on sperm morphology, sister chromatid exchanges or on micronuclei formation in rabbits (1982)
    Springer
    Archives of toxicology 50 (1982), S. 149-157 
    Details
    ISSN: 1432-0738
    Schlagwort(e): Lead ; Sperm ; SCE ; Micronuclei ; Rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The influence of lead on sperm morphology, sister chromatid exchanges or on micronuclei formation was studied on male rabbits after exposure to doses of 0, 0.25, and 0.50 mg lead acetate/kg body weight subcutaneously injected three times a week during 14 weeks, each on a group of five rabbits. At the end of exposure phase the lead in blood concentrations of the three groups of rabbits were 0.32, 2.57, and 2.97 μmol/l respectively. The results did not show any evidence of treatment related effects on sperm count or on morphologic abnormalities of the sperms, neither on the histopathology of the testis. Statistical analysis of the number of sister chromatid exchanges per metaphase in lymphocytes indicated no differences between the groups. Also no dose dependent effect was observed on the relative number of micronuclei in bone marrow erythrocytes. The different susceptibility to lead in different organ systems of the rabbits was discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00373397
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  • 2
    Digitale Medien
    Digitale Medien
    A new method for the enzyme cytochemical staining of individual cells with the use of a polyacrylamide carrier (1982)
    Springer
    Histochemistry and cell biology 74 (1982), S. 171-181 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A new method for enzyme cytochemical studies on individual cells is developed. Cells are incorporated in the matrix of a thin film of transparent polyacrylamide prior to incubation in a cytochemical medium. Five different kinds of individual cells, i.e. isolated rat hepatocytes, isolated mouse oocytes, cultivated human fibroblasts, rat thymocytes and human blood cells are used for testing the applicability of this method for the cytochemical demonstration of glucose-6-phosphate dehydrogenase with tetranitro BT. The incorporation technique solves at least some of the problems occurring with enzyme cytochemistry on single cells. The morphology of the cells is very well preserved, the formazan precipitation due to enzyme activity occurs entirely within the cell cytoplasm, the nothing dehydrogenase activity can be kept very low and the loss of cells is completely prevented with all cell types used.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00495828
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  • 3
    Digitale Medien
    Digitale Medien
    Age-related changes of glucose-6-phosphate dehydrogenase activity in mouse oocytes (1987)
    Springer
    Journal of molecular histology 19 (1987), S. 467-470 
    Details
    ISSN: 1573-6865
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Glucose-6-phosphate dehydrogenase (G6PDH) activity was measured in follicular oocytes and in ovulated eggs of prepubertal, adult and aged mice. G6PDH activity in ovulated eggs was 60% of the activity in follicular oocytes in all age groups. The mean G6PDH activity was significantly higher in follicular oocytes of adult mice than in oocytes of both prepubertal and aged mice. In aged mice, the decreased mean activity in follicular oocytes as well as in ovulated eggs was mainly due to a high percentage of cells with extremely low activity (25 and 18%, respectively). The percentage of preovulatory oocytes with low activity in prepubertal mice was 9% and in adult mice 0.3%. For ovulated eggs these percentages were 0% for both prepubertal and adult mice. In every age group, all ovulated eggs showed a normal morphology. When ovulated eggs with extremely low G6PDH activity can still be fertilized, it can be questioned whether this loss of activity could cause disturbances in development of (preimplantation) embryos. Our findings emphasize the potentialities of investigating intact single oocytes for changes in enzyme activities, which could be applied as parameters for ‘quality control’ of these cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01675415
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  • 4
    Digitale Medien
    Digitale Medien
    In situ glucose-6-phosphate dehydrogenase activity during development of pre-implantation mouse embryos (1993)
    Springer
    Journal of molecular histology 25 (1993), S. 299-303 
    Details
    ISSN: 1573-6865
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Glucose-6-phosphate dehydrogenase activity was analysed cytophotometrically in oocytes and pre-implantation embryos of mice. A bimodal distribution pattern was not found. Therefore, female and male embryos could not be discriminated on the basis of linkage of the enzyme with the X-chromosome during the pre-implantation period. The dehydrogenase activity in ovulated eggs and pre-implantation embryos up to the 8-cell stage was 65% of that present in follicular oocytes. In morulae and blastulae, the activity was further decreased to a level that was only 10–20% of the activity present in oocytes. The dramatic decrease in dehydrogenase activity could not be explained by modulation of the enzyme molecules, because K M values did not vary strongly. It is unlikely that the abundant activity of glucose-6-phosphate dehydrogenase in oocytes is due to high activity of the pentose phosphate pathway because of the low activity of 6-phosphogluconate dehydrogenase, the next step in this pathway. It is concluded that high activity of glucose-6-phosphate dehydrogenase in oocytes is needed for keeping oocytes viable, and for generation of NADPH which is important for the fertilization process.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00159121
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