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  • 1
    Electronic Resource
    Electronic Resource
    Localization of Mg++-dependent adenosine triphosphatase and alkaline phosphatase activities in the postimplantation mouse embryos in day 5 and 6 (1985)
    Springer
    Anatomy and embryology 173 (1985), S. 7-11 
    Details
    ISSN: 1432-0568
    Keywords: Postimplantation mouse embryos ; Mg++-dependent adenosine triphosphatase ; Alkaline phosphatase ; Ultracytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mg++-dependent adenosine triphosphatase (Mg-ATPase) and alkaline phosphatase (ALPase) activities were histo- and cytochemically investigated in postimplantation mouse embryos from day 5 to day 6. In day 5 postimplantation embryos, Mg-ATPase activity was detected in the embryonic ectoderm and weakly in the visceral endoderm. Weak ALPase activity was found in the embryonic ectoderm and visceral endoderm. Parietal endoderm, both in day 5 and in day 6 embryos, had very weak or no Mg-ATPase and ALPase activities. Mg-ATPase activity in day 6 embryos was found with the same localization as that in day 5 embryos. No ALPase activity was observed in their embryonic ectoderm. Extraembryonic ectodermal cell mass had the strongest Mg-ATPase activity in these stage embryos. These results suggest that the localization of both enzyme activities in postimplantation mouse embryos is closely related to the morphogenesis. As regards the proamniotic cavity formation, the fact that Mg-ATPase activity was still observed in the embryonic ectoderm in these stages suggests the involvement of active transport system on the production of nascent proamniotic cavity fluid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00707299
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  • 2
    Electronic Resource
    Electronic Resource
    A simple approach for the analysis of intracellular movement of oxidant-producing intracellular compartments in living human neutrophils (2000)
    Springer
    Histochemistry and cell biology 113 (2000), S. 251-257 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In human neutrophils, superoxide is generated primarily within specialized oxidant-producing intracellular compartments. The present study employs a simple methodological approach to evaluate the intracellular movement of these structures in living human neutrophils. Using a CCD camera system, we monitored fluorescence in cells loaded with the succinimidyl ester of dichlorodihydrofluorescein diacetate, which is non-fluorescent until oxidized by reactive oxygen species. Fluorescence-positive intracellular compartments became detectable after neutrophils were stimulated with phorbol myristate acetate for 1 min. Further stimulation increased the intracellular compartments in both number and size in a time-dependent manner. Upon stimulation with phorbol myristate acetate, no fluorescence was seen in intracellular compartments of neutrophils isolated from patients with X-linked chronic granulomatous disease lacking gp91-phox, a membrane component of NADPH oxidase. The method enables tracking of the movement of a single oxidant-producing intracellular compartment following cell stimulation and visualization of the intracellular structures formed by fusion of oxidant-producing intracellular compartments with endocytotic vesicles and phagosomes. Therefore, it is considered to be an informative tool for evaluation of the intracellular dynamics of oxidant-producing intracellular compartments in living human neutrophils and may have a diagnostic value.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180000132
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  • 3
    Electronic Resource
    Electronic Resource
    Dynamics of rat liver ecto-ATPase during development suggests its involvement in bile acid efflux (1999)
    Springer
    Cell & tissue research 298 (1999), S. 511-518 
    Details
    ISSN: 1432-0878
    Keywords: Extracellular ATP Ecto-ATPase Signal transduction Liver Development Bile acid efflux Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We studied cytochemical localization of ectoadenosine triphosphatase in the rat liver during development from 15-day-old fetus to 4-week-old and adult animal. First signs of the enzyme activity were found in some of the primitive bile canaliculi of 15-day-old fetuses. The majority of canaliculi, however, did not reveal any reaction product. Although intensity of the cytochemical reaction increased at 20 days of gestation, it still remained relatively low. Intensity of the reaction increased significantly and its product became readily detectable in the liver of newborn rats. Liver of 1-, 2-, and 4-week-old animals showed strong reaction for ecto-ATPase at the luminal surface of the plasma membrane of the bile canaliculi. Liver of adult rats contained a prominent reaction product similar to that seen in newborns, 1-, 2-, and 4-week-old animals. At all stages of fetal development, as well as in postnatal and adult rats, reaction was found only within the hepatic bile canalicular system and exclusively at the luminal surface of the canalicular plasma membrane. Using diethyl pyrocarbonate (DEPC), a specific inhibitor of ecto-ATPase activity, cytochemical reaction was blocked in all examined samples. Results of the present study, taken together with established biochemical and immunological data, provide conclusive morphological evidence in support of the view that canalicular ecto-ATPase is involved in bile acid efflux.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004419900065
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of Oxidant Producing-sites in Glutaraldehyde-fixed Human Neutrophils and Eosinophils Stimulated with Phorbol Myristate Acetate (1999)
    Springer
    Journal of molecular histology 31 (1999), S. 181-194 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The aim of the present study was to detect oxidant-producing sites, and to elucidate their dynamic reorganization in human polymorphonuclear leukocytes (PMNs) fixed with glutaraldehyde which preserves cell structure. In biochemical analyses, the detectable O2− generation in unfixed PMNs upon stimulation with phorbol 12-myristate 13-acetate (PMA) in the presence of cytochalasin B was characterized by a lag period of approximately 10 sec followed by O2− production. The maximal rate reached was 3.18±0.07 n mol/min/1×106 cells (mean±S.D.; n=4) after 30 sec of stimulation. PMNs exposed to PMA and cytochalasin B followed by fixation with glutaraldehyde generated O2− without a lag period at a rate of 0.35±0.05 n mol/min/1×106 cells (mean±S.D.) by the addition of NADPH as substrate to the cell suspension. In the cytochemical assays, we employed both cells exposed to PMA and cytochalasin B, and then fixed with glutaraldehyde followed by incubation in the cytochemical reaction medium (pre-fixed cells) and cells incubated in the medium containing PMA and cytochalasin B followed by fixation with glutaraldehyde (post-fixed cells). Oxidant reaction in the pre-fixed cells was detected by the addition of NADPH and FAD to the reaction medium. No oxidant-reaction product was seen in pre-fixed cells stimulated for 10 sec whereas the oxidant reaction was visualized in intracellular compartments of pre-fixed PMNs stimulated for 20 sec. The fact that the pre-fixed PMNs stimulated for 30 sec showed increased numbers of oxidant-producing structures compared to those seen in the pre-fixed cells stimulated for 20 sec, demonstrates that the amount of the reaction product and the number of oxidant-producing intracellular compartments increases between 20 and 30 sec after start of stimulation with PMA. These cytochemical results using the pre-fixed cells coincided with the findings obtained from the biochemical assays in the pre-fixed cells exposed to PMA and cytochalasin B. The oxidant reaction was observed in elongated tubular structures that were arranged in a radial fashion, and were associated with the plasma membrane in the pre-fixed PMNs, whereas post-fixed PMNs exhibited slender spherical or rod-shaped structures of various lengths. The present results indicate that the pre-fixed PMNs can be employed for elucidating the dynamic reorganization of oxidant-producing sites in human PMNs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003547121574
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  • 5
    Electronic Resource
    Electronic Resource
    The confronting cisternae in neurons in the substantia gelatinosa of the spinal cord of rats and Japanese monkeys (1983)
    Springer
    Cell & tissue research 232 (1983), S. 487-492 
    Details
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Substantia gelatinosa ; Adenosine triphosphatase, sodium-potassium ; Thiamine pyrophosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurons in the substantia gelatinosa of the spinal cord of the rat and Japanese monkey were investigated electron microscopically and ultracytochemically. The confronting cisternae observed in the cytoplasm of the majority of gelatinosal neurons in both species were composed of closely apposed parallel cisternae with electron-dense flocculent material, and a continuity with the cisternae of the rough endoplasmic reticulum was often observed. Ultracytochemically, the p-nitrophenylphosphatase (p-NPPase) activity was present in the confronting cisternae, but thiamine monophosphatase (TMPase) and thiamine pyrophosphatase (TPPase) activities were absent. These results indicate that the confronting cisternae are a variant of the rough endoplasmic reticulum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216423
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