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  • 1
    ISSN: 1600-065X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary: During B-cell development the surrogate light (SL) chain is selectively expressed in progenitor and precursor B cells during the developmental stages of DH to JH and VH to DH JH rearrangements. Approximately half of all H chains produced by these rearrangements cannot pair with SL chains and cannot form a pre-B-cell receptor (pre-BCR). A spectrum of affinities between VpreB and individual VH domains generates preB cells with pre-BCR of different fitness which, in turn, determines the extent of the pre-B II-cell proliferation and the fidelity of allelic exclusion of the H chain locus. Once pre-BCR is expressed, SL chain expression is turned off. As pre-B II cells proliferate, SL is diluted out, thus limiting pre-BCR formation. As a consequence, pre-B II cells stop proliferating, become small and resting and begin to rearrange the L chain loci. Multiple rearrangements of the k L chain alleles are often detected in wild-type small pre-B II cells. Around 20% of the H chain-expressing small pre-B II cells also express L chains but do not display the Ig on the surface. Hence, it is likely that not all L chains originally generated in resting pre-B II cells can pair with the H chain previously present in that cell. The best fitting ones are selected preferentially to generate sIg+ B cells. Furthermore, the transition of immature B cells from the bone marrow to spleen and their development to mature cells appear as two separate steps controlled by different genes.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Informatik-Spektrum 22 (1999), S. 376-378 
    ISSN: 1432-122X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Geoinformatica 2 (1998), S. 113-147 
    ISSN: 1573-7624
    Keywords: approximation-based similarity search ; multi-step similarity query processing ; ellipsoid queries on multidimensional index structures ; 3-D spatial database systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract The issue of finding similar 3-D surface segments arises in many recent applications of spatial database systems, such as molecular biology, medical imaging, CAD, and geographic information systems. Surface segments being similar in shape to a given query segment are to be retrieved from the database. The two main questions are how to define shape similarity and how to efficiently execute similarity search queries. We propose a new similarity model based on shape approximation by multi-parametric surface functions that are adaptable to specific application domains. We then define shape similarity of two 3-D surface segments in terms of their mutual approximation errors. Applying the multi-step query processing paradigm, we propose algorithms to efficiently support complex similarity search queries in large spatial databases. A new query type, called the ellipsoid query, is utilized in the filter step. Ellipsoid queries, being specified by quadratic forms, represent a general concept for similarity search. Our major contribution is the introduction of efficient algorithms to perform ellipsoid queries on multidimensional index structures. Experimental results on a large 3-D protein database containing 94,000 surface segments demonstrate the successful application and the high performance of our method.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0749-503X
    Keywords: cell wall ; mannosylation ; KEX2 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Intact Saccharomyces cerevisiae cells were biotinylated with the non-permeable sulfosuccinimidyl-6-(biotinamido)hexanoate reagent. Twenty specifically labelled cell wall proteins could be extracted and visualized on SDS gels via streptavidin/horseradish peroxidase. Nine cell wall proteins were released by SDS extraction under reducing conditions and were designated Scw1-9p for (soluble cell wall proteins); five proteins were released from SDS-extracted cell walls by laminarinase (Ccw1-5p for covalently linked cell wall proteins) and six with mild (30 mm-NaOH, 4°C, 14 h) alkali treatment (Ccw6-11p). N-terminal sequences of the Ccw proteins 6, 7, 8 and 11 showed that these cell wall proteins are members of the PIR gene family (predicted proteins with internal repeats), CCW6 being identical to PIR1 and CCW8 to PIR3. Single gene disruptions of all four genes did not yield a phenotype. In the CCW11 disruption the Ccw11p as well as the laminarinase-extracted Ccw5 protein was missing. The new cell wall proteins are O-mannosylated, contain a Kex2 processing site, but no C-terminal GPI anchor sequence. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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