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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemistry and Physics of Lipids 17 (1976), S. 336-343 
    ISSN: 0009-3084
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Pituitary ; Teleost ; GTH-cells ; Innervation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the male black molly, Poecilia latipinna, morphological and functional aspects of the gonadotropic (GTH-)cells have been studied at the ultrastructural level. The cells exclusively occupy the ventral and lateral areas of the meso-adenohypophysis. In the black molly there is evidence of the presence of only one type of gonadotropic cell. In the GTH-cells of most specimens, the rough endoplasmic reticulum is weakly developed. The secretory vesicles are characterized by cores with varying diameters; this variation was not observed in the secretory vesicles of the other types of pituitary cells, except in the TSH-cells. After applying a histochemical method for the demonstration of polysaccharides, small black deposits appear in the core of the secretory vesicles of the GTH and TSH-cells only; this indicates the glycoproteinaceous nature of the hormones produced in these cells. Male black mollies treated with methyl-testosterone have significantly smaller GTH-cells and a lesser number of secretory vesicles and mitochondria in these cells. GTH-cell activity in Poeciliinae may be thus influenced by androgens by means of a negative feed-back mechanism. The GTH-cells are innervated by both type A and type B neurosecretory fibres. There are indications that the type A fibres may originate from the pars lateralis cells of the nucleus lateralis tuberis; the origin of the type B fibres is uncertain.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Melanophores ; Xenopus laevis ; Tadpole ; Cell culture ; Melanophore-stimulating hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Melanophores from tadpoles of Xenopus laevis (Daudin) were isolated by digestion of tail fins with acetyltrypsin and collagenase and maintained in primary culture for 6 weeks up to 3 months. Within 36 to 72 h the melanophores develop one to eight dendritic processes per cell; secondary and tertiary branchings of the processes were frequently observed. The melanophores in primary culture disperse under the influence of α-MSH or cyclic AMP; upon rinsing out these substances the cells aggregate. In darkness, about 40 % of the cells disperse their pigment, whereas under illumination the pigment of the melanophores aggregates. To date, attempts to initiate cell division in melanophores have not been successful.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Melanophores ; Pigment migration ; Xenopus laevis ; Cell culture ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tail-fin melanophores of tadpoles of Xenopus laevis (Daudin) in primary culture were examined scanning electron microscopically in the aggregated and in the dispersed state. After isolation, the melanophores are spherical, but within 24 h they develop thin filopodia for attachment to the substratum. Subsequently, cylinder-like as well as flat sheet-like processes are formed, which adhere to the substratum with terminal pseudopodia and filopodia. The processes of adjacent melanophores contact each other, thus forming an interconnecting network between the melanophores. In the aggregated state the central part of the melanophore is spherical and voluminous. Both the central part and the processes bear microvilli. In melanophores with dispersed melanosomes the central part is much flatter; the distal parts have a thickness that equals a monolayer of melanosomes. The surface of the cell bears only scarce microvilli. These features indicate that melanophores do not have a fixed shape and that pigment migration is accompanied by reciprocal volume transformation between the cell body and its processes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0878
    Keywords: Melanophores ; Periodic albinism ; Ultrastructure ; Physiology ; Xenopus laevis, tadpoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pigment of tail-fin melanophores in periodic albino Xenopus laevis tadpoles is dispersed in response to darkness and to α-MSH in a manner similar to wild-type melanophores. However, periodic albino tadpoles lack the response to different background conditions and the melatonin-induced aggregation in darkness. The tyrosinase activity in cells of the latter type tadpoles is weak compared to the wild-type cells. Ultrastructural examination of melanophores from periodic albino mutants and cells from wild-type tadpoles shows similar organelles at corresponding sites. A morphological difference can be observed in the fine structure of the melanosomes, which in albinos resembles an earlier stage of development. It is postulated that periodic albino Xenopus laevis possess the cellular mechanism to disperse pigment in the melanophores, but that under physiological conditions the release of α-MSH appears to be absent or scarce.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0878
    Keywords: Teleosts ; Pituitary ; GTH ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using anti-carp-gonadotropic-γ-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong fluorescence was observed in the ventralmost region, containing the presumed gonadotropic cells; weak fluorescence was observed in the dorsal region, in the presumed thyrotropic cells. The possibility of an unspecific reaction with TSH-cells in the latter region is discussed. Treatment of male black mollies for 38 days with methyltestosterone resulted in a loss of fluorescence in the ventral region of the meso-adenohypophysis, whereas there was no decrease of the fluorescence in the dorsal region. The results support the hypothesis that the ventral basophils in the mesoadenohypophysis produce gonadotropic hormone.
    Type of Medium: Electronic Resource
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