ISSN:
0377-0486
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
Derivative Stokes-Raman spectroscopy has been applied to biomembrane systems containing the fluorescent antibiotic amphotericin B. The membranes studied included weakly scattering solutions of dimyristoylphosphatidylcholine (DMPC) and multilamellar liposomes composed of DMPC and cholesterol. In this techqnique, the wavelength of an Ar ion-laser pumped, tunable CW Rhodamine 6G dye laser was sinusoidally modulated with an electro-optic birefringent tuner. The wavelength-modulated Raman signal becomes amplitude-modulated at the exit slit of a scanning monochromator. The signal, demodulated by a phase-sensitive detector, was proportional to the first derivative of the Raman spectrum under appropriate conditions. The results indicate that this modulation technique can offer improvements in Raman signal-to-noise ratios of up to a factor of two for the conformationally sensitive phospholipid acyl-chain vibrations of the biomembranes.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jrs.1250150204
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