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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 13 (1981), S. 15-18 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The equilibrium oxygen concentration (C0) was determined in a complex fermentation media containing sucrose, lysine, molasses, corn steep liquor, antifoam agents and biomass. In simple systems, with all the components being dissolved, C0 represents the oxygen solubility and linearly decreases with increasing solute concentrations. In complex solutions with multi-phase structure an increase in C0 can be detected. It suggests that C0 consists of two components — one being oxygen solubility, the other being determined by the amount of oxygen adsorbed on the interphase and bound by macromolecules. The presence of biomass leads to a decrease in C0.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The present paper deals with the coordination of energy metabolism, glucose consumption rate, glycolytic and TCA cycle enzyme activities in the lysine-producing bacterium Brevibacterium flavum. It is shown, that inhibition of the elctron transport chain causes changes of the following sequence: at first, TCA cycle enzymes are activated; secondly, TCA cycle enzyme activity decreases, and glycolytic enzyme activities as well as glucose transport rate increase; there is a slight increase in Qo2 and a considerable one of O2 consumption in cyanide-resistant respiration pathway; thirdly, TCA cycle enzyme activities and glucose transport rate decrease. It is supposed, that coordination of carbon and energy metabolism in B. flavum depends on intracellular ATP concentration or energy charge value.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 897-912 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A scheme for lysine biosynthesis using variants of the Brevibacterium flavum intermediary metabolite synthesis is discussed. The main precursor of lysine that we are concerned with here is oxalacetate, which can be synthesized through the TCA or glyoxylate cycles or by carboxylation of PEP. Material energy balances for the main pathways of lysine biosynthesis from glucose and acetate have been formulated. Energy consumption, in the from of ATP - PATP (number of mol ATP consumed/1 mol lysine synthesized), was calculated for the main pathways of lysine biosynthesis. Theoretical conversion yields Ypmax (g product/g substrate) were estimated. Experimental data were presented concerning the increase of Yp by means of metabolism regulation: (a) by TCA-and glyoxylate-cycle enzyme induction; (b) by maintaining PEP carboxylase activity; (c) by eliminating by-product synthesis.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 421-430 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The efficiency of carbon substrate utilization is evaluated as the thermodynamic efficiency (ηx) of microbial growth.Three methods based on mass-energy balance are used for the efficiency studies of complex substrates (straw, plant juices, lye) utilization by microfungi Trichoderma viride.1According to substrate and biomass balance ηx = 0.55, 0.37 and 0.36 for Trichoderma viride growth on alkali pretreated wheat straw during 23, 34 and 50 hours. Cellulose biodegradation increases with cultivation time. However, the efficiency of cellulose utilization for cell mass growth decreases at the same time.2In accordance with oxygen-balance calculations ηx = 0.75 and 0.71 for the same processes. The discrepancy in results from the above two methods probably can be explained by the following: ASubstrate and biomass balance gives underestimated results.BOxygen balance method includes the part of energy for extracellular product formation and therefore ηx can be overestimated.CThe efficiency of complex soluble substrate utilization (lye, green juice, deproteinized brown plant juice) tested by means of pulse method gives the values of ηx = 0.72 - 0.88. Similar high estimates of ηx in C-limited batch culture are observed for soluble carbohydrates (glucose, galactose, lactose, xylose) but not for acetate. The pulse method is advantageous for testing the “true” efficiency of carbon substrate utilization in a definite physiological environment.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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