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  • 1
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Glycerol-3-phosphate 1-acyltransferase (E.C. 2.3.1.15; G3PAT) catalyses the incorporation of an acyl group from either acyl-acyl carrier proteins (acylACPs) or acylCoAs into the sn-1 position of glycerol 3-phosphate to yield 1-acylglycerol 3-phosphate. Crystals of squash G3PAT have been obtained by the hanging-drop method of vapour diffusion using PEG 4000 as the precipitant. These crystals are most likely to belong to space group P212121, with approximate unit-cell parameters a = 61.1, b = 65.1, c = 103.3 Å, α = β = γ = 90° and a monomer in the asymmetric unit. X-ray diffraction data to 1.9 Å resolution have been collected in-house using a MAR 345 imaging-plate system.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 24 (1994), S. 21-34 
    ISSN: 1573-5028
    Keywords: acetyl CoA carboxylase ; biotin ; bovine serum albumin ; cDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat germ acetyl CoA carboxylase (ACCase) was purified by liquid chromatography and electroelution. During purification bovine serum albumin (BSA) was used to coat Amicon membranes used to concentrate partially pure ACCase. Despite further SDS-PAGE/electroelution and microbore HPLC steps BSA remained associated. This presented serious protein sequencing artefacts which may reflect the affinity of BSA for fatty acids bound to ACCase. To avoid these artefacts the enzyme was digested in gel with Endoproteinase LysC protease without the presence of BSA, and the resulting peptides blotted and sequenced. A partial cDNA (1.85 kb) encoding ACCase from a wheat embryo library was cloned, which hybridised to a 7.5 kb RNA species on northern blot of wheat leaf poly(A)+ RNA. The partial cDNA therefore represents about 0.25 of the full-length cDNA. The clone was authenticated by ACCase peptide sequencing and immuno cross-reactivity of the overexpressed clone. The derived amino acid sequence showed homology with both rat and yeast ACCase sequences (62%). Antibodies raised against wheat acetyl CoA carboxylase were specific for a 220 kDa protein from both wheat embryo and leaf. In addition, by using a novel quick assay for ACCase that utilised 125I-streptavidin, we showed the major biotin containing protein to be 220 kDa in both leaf and germ. This is in marked contrast to the previously published molecular mass of 75 kDa allocated to wheat leaf ACCase.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5117
    Keywords: Bangladesh ; deepwater rice ; Photosynthetically Active Radiation ; water chemistry ; oxygen ; phosphate ; sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This introduces a series of five papers describing physical, chemical and biological features of Bangladesh deepwater rice-fields. Flooding occurs typically from late June to early November. There were two distinct flood peaks (late July, mid-September) at the two main research sites during each of the years 1981–86, with maximum depths reaching about 1.5–2.0 m. Temperature showed a narrow range, with values for the upper part of the water column during July–October seldom outside the range 29–35 °C. Data for PAR (Photosynthetically Active Radiation) obtained with two types of sensor (4 π and cosine) are summarized, with examples of profiles and changes throughout the day. Water chemistries sampled from 13 different locations at various times during 1981–1983 are compared for: conductivity, pH, total alkalinity, O2, major metals (Na, K, Mg, Ca, Mn, Fe), minor metals (Co, Ni, Cu, Zn, Cd, Pb), NH4-N, NO2-N, NO3-N, filtrable reactive P, filtrable organic P, non-filtrable P, Cl, SO4-S, reactive-Si. Overall the concentrations of aqueous N and P may be regarded as providing a mesotrophic environment for algae, but with relatively low N : P. Filtrable organic P was at least as important as filtrable reactive P, with mean values at the various locations from 0.020 to 0.036 mg l−1 P for the former and 0.005 to 0.038 mg l−1 for the latter. Measurements of N and P in the water column at any one time typically gave values (expressed per unit area) slightly lower than totals for these elements deposited in sediments throughout the whole season. At least until the end of September, the upper part of the water column was almost always oxic during the daytime and occasionally supersaturated with O2 in the afternoon. However DWR fields in south Bangladesh (Phaltita) visited on a sunny day in late September had waters which were almost entirely anoxic even in the afternoon: channels were lacking here and rotting rice stems from an earlier crop were widespread.
    Type of Medium: Electronic Resource
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