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  • 1
    Electronic Resource
    Electronic Resource
    Two-dimensional poly(acrylamide) electrophoresis of fluoresceinated glycopeptides. Resolution and structural characterization of ovalbumin glycans
    Amsterdam : Elsevier
    Carbohydrate Research 236 (1992), S. 1-8 
    Details
    ISSN: 0008-6215
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0008-6215(92)85002-H
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The (1 -〉 3)-linked α-l-fucosyl group of the N-glycans of the Wistaria floribunda lectins is recognized by a rabbit anti-serum
    Amsterdam : Elsevier
    Carbohydrate Research 213 (1991), S. 27-36 
    Details
    ISSN: 0008-6215
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0008-6215(00)90595-0
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic deficiency of human mast cell a-tryptase (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Human α- and β-tryptases are proteases secreted by mast cells. Beta (but not α) tryptases are implicated in asthma. Genes encoding both types of tryptases cluster on chromosome 16p13.3.Objective This study examines the hypothesis, generated from mapping data, that α-alleles compete with some β-alleles at one locus and that an adjacent locus contains β-alleles exclusively. This hypothesis predicts that β-alleles outnumber α and that some genomes lack α genes altogether.Methods To test this hypothesis, we developed PCR-based techniques to distinguish α from β genes. We then genotyped genomic DNA from individuals and tryptase-expressing cell lines.Results In support of our hypothesis, we find that α-tryptase deficiency affects 80/274 (29%) of individuals surveyed. The genotype of the α-deficient individuals is ββββ, due to inheritance of four β genes. The percentage of the population with the mixed genotypes ααββ and αβββ is 21% and 50%, respectively. Accounting for all α- and β-alleles at the tandem loci on 16p13.3, overall α-allele frequency is only 0.23, with β-alleles considerably outnumbering α as hypothesized. In samples of defined ethnicity, α deficiency affects 45% of Caucasians, but a much lower percentage of other backgrounds, including African-Americans and Asians. Examination of cell lines reveals that HMC-1 and U-937 lack α-genes; thus, lack of α transcripts in these cells is due to absence of α-genes rather than β-selective transcription. By contrast, α-transcribing Mono Mac 6 and KU812 cells contain α- and β-genes.Conclusions Genetic α-tryptase deficiency is common and varies strikingly between ethnic groups. Because β-tryptases are implicated in allergic disorders, inherited differences in α/β-genotype may affect disease susceptibility, severity and response to tryptase inhibitor therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2002.01416.x
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  • 4
    Electronic Resource
    Electronic Resource
    Granulosa cell aromatase bioassay: Changes of bioactive FSH levels in the female
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 33 (1989), S. 721-726 
    Details
    ISSN: 0022-4731
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(89)90483-4
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  • 5
    Electronic Resource
    Electronic Resource
    Evaluation of salmon farming effects on marine systems in the inner seas of southern Chile: a large-scale mensurative experiment (2004)
    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 20 (2004), S. 0 
    Details
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The impact of salmon farming on the environment has been widely studied; there is, however, no consensus on the magnitude and quality of these effects and little information on their extent over large salmon farming areas. Forty-three salmon farm sites of which 29 are in full operation and grouped in nine locations in southern Chile were evaluated. Using statistical methods (two-way anova), no effects were found on water column variables such as nitrate, ammonia, orthophosphate and chlorophyll, whereas they were significant on sediment variables such as nitrogen (N), phosphorus (P) and organic carbon (OC), among others. Natural variability evaluation of some parameters revealed that P in sediments had the lowest coefficient of variation (23.2%) when compared with N (65.2%), particulate organic matter (POM) (139%) and OC (39%), and showed more marked salmon farming effects with no locality effects. Four of the nine locations showed stronger effects on sediments, with average P values ranging from 150 to 230 mmol that were six to nine times higher than in control sites (25 mmol). Nitrogen and carbon in sediments, while showing significant effects on salmon farming, also showed locality effects that revealed other biogenic processes influencing sediment composition. Thus, P in sediments was proposed as a promising indicator of impact on salmon farming, although the relationship with fauna in sediments was not linear and somewhat variable; more research is therefore needed to understand such connections. Considering entire geographical locations, no relationship was found between sediment conditions under salmon cages and the condition of the water column at a farm. This may indicate the possibility of high dilution rates and recycling processes, which so far preclude the detection of more global impacts beyond the cages shadow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0426.2004.00602.x
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  • 6
    Electronic Resource
    Electronic Resource
    The impact of accelerating land-use change on the N-Cycle of tropical aquatic ecosystems: Current conditions and projected changes (1999)
    Springer
    Biogeochemistry 46 (1999), S. 109-148 
    Details
    ISSN: 1573-515X
    Keywords: estuaries ; lakes ; marine ; nitrogen ; phosphorus ; rivers ; streams ; temperate ; tropics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Published data and analyses from temperate and tropical aquatic systems are used to summarize knowledge about the potential impact of land-use alteration on the nitrogen biogeochemistry of tropical aquatic ecosystems, identify important patterns and recommend key needs for research. The tropical N-cycle is traced from pre-disturbance conditions through the phases of disturbance, highlighting major differences between tropical and temperate systems that might influence development strategies in the tropics. Analyses suggest that tropical freshwaters are more frequently N-limited than temperate zones, while tropical marine systems may show more frequent P limitation. These analyses indicate that disturbances to pristine tropical lands will lead to greatly increased primary production in freshwaters and large changes in tropical freshwater communities. Increased freshwater nutrient flux will also lead to an expansion of the high production, N- and light-limited zones around river deltas, a switch from P- to N-limitation in calcareous marine systems, with large changes in the community composition of fragile mangrove and reef systems. Key information gaps are highlighted, including data on mechanisms of nutrient transport and atmospheric deposition in the tropics, nutrient and material retention capacities of tropical impoundments, and N/P coupling and stoichiometric impacts of nutrient supplies on tropical aquatic communities. The current base of biogeochemical data suggests that alterations in the N-cycle will have greater impacts on tropical aquatic ecosystems than those already observed in the temperate zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006156213761
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The impact of accelerating land-use change on the N-Cycle of tropical aquatic ecosystems: Current conditions and projected changes (1999)
    Springer
    Biogeochemistry 46 (1999), S. 109-148 
    Details
    ISSN: 1573-515X
    Keywords: estuaries ; lakes ; marine ; nitrogen ; phosphorus ; rivers ; streams ; temperate ; tropics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Published data and analyses from temperate and tropical aquatic systems are used to summarize knowledge about the potential impact of land-use alteration on the nitrogen biogeochemistry of tropical aquatic ecosystems, identify important patterns and recommend key needs for research. The tropical N-cycle is traced from pre-disturbance conditions through the phases of disturbance, highlighting major differences between tropical and temperate systems that might influence development strategies in the tropics. Analyses suggest that tropical freshwaters are more frequently N-limited than temperate zones, while tropical marine systems may show more frequent P limitation. These analyses indicate that disturbances to pristine tropical lands will lead to greatly increased primary production in freshwaters and large changes in tropical freshwater communities. Increased freshwater nutrient flux will also lead to an expansion of the high production, N- and light-limited zones around river deltas, a switch from P- to N-limitation in calcareous marine systems, with large changes in the community composition of fragile mangrove and reef systems. Key information gaps are highlighted, including data on mechanisms of nutrient transport and atmospheric deposition in the tropics, nutrient and material retention capacities of tropical impoundments, and N/P coupling and stoichiometric impacts of nutrient supplies on tropical aquatic communities. The current base of biogeochemical data suggests that alterations in the N-cycle will have greater impacts on tropical aquatic ecosystems than those already observed in the temperate zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007576
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    Paper (German National Licenses)
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