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  • 1
    Electronic Resource
    Electronic Resource
    Effect of 2-deoxyglucose, α-methylglucoside, and glucosamine on aflatoxin production by Aspergillus parasiticus (1985)
    Springer
    Archives of microbiology 142 (1985), S. 200-203 
    Details
    ISSN: 1432-072X
    Keywords: Aflatoxins ; Aspergillus parasiticus ; Glucose analogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of 2-deoxyglucose (2-DOG), α-methylglucoside (α-MG), and glucosamine (GA) on aflatoxin production by Aspergillus parasiticus were studied using conidia-initiated and replacement cultures. In conidia-initiated, 2-DOG, α-MG, and GA supported varying amounts of growth when employed as sole carbon sources. In both conidia-initiated and replacement cultures, 2-DOG, but not α-MG nor GA, as sole carbon sources support toxin formation. None of the compounds inhibited aflatoxin production when used in combination with glucose. It appears that neither 2-DOG, α-MG, nor GA can be considered nonmetabolizable analogs of glucose in A. parasiticus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447068
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Further Characterization of a Caffeine-Resistant Mutant of Aspergillus parasiticus (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies were performed to characterize further Aspergillus parasiticus BCR1, a caffeine-resistant mutant of A. parasiticus NRRL 2999, particularly in regard to its caffeine-dependent production of aflatoxins. The enhanced synthesis of aflatoxins by caffeine was highly specific since neither dimethylxanthines nor purines could substitute for the trimethylxanthine. Caffeine's effects were phase dependent and only increased toxin formation if added early in the microorganism's life cycle. The ability of BCR1 to exclude caffeine appeared dependent on the initial levels of caffeine in the growth medium. Respiration and glucose utilization in the wild type strain were inhibited strongly by caffeine, but BCR1 was resistant to these effects. Comparison of glucose uptake kinetics in the wild type and mutant strains indicated that caffeine inhibition of aflatoxin synthesis in the wild type was not due to a disruption of glucose transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb07861.x
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of a Caffeine-Resistant Mutant of Aspergillus parasiticus: Role of Amino Acid Metabolism (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 52 (1987), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aspergillus parasiticus BCRI, a caffeine-resistant mutant of A. parasiticus NRRL 2999, produced abundant amounts of aflatoxins (AF) in yeast extract-sucrose broth only when the medium was supplemented with caffeine. However, little AF production occurred in glucose-mineral salts medium (GMS) regardless of the level of caffeine supplementation. Caffeine-dependent AF production was restored if GMS were fortified with peptone or some other source of amino acids. Subsequent studies indicated that this effect could be achieved by supplementing GMS with specific amino acids, particularly proline, alanine, methionine, arginine or asparagine. Restoration of caffeine-dependent AF synthesis did not occur when GMS was supplemented with purine bases or nucleotides. The results indicated that caffeine-dependent AF production in BCRI was dependent on amino acid catabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1987.tb05912.x
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  • 4
    Electronic Resource
    Electronic Resource
    ABILITY OF VARIOUS CARBON SOURCES TO INDUCE AND SUPPORT AFLATOXIN SYNTHESIS BY ASPERGILLUS PARASITICUS (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 6 (1984), S. 0 
    Details
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability of a wide variety of carbon sources to induce and support aflatoxin synthesis by Aspergillus parasiticus was examined using mycelia pregrown in a peptone-mineral salts medium that does not support aflatoxin synthesis. Sugars and derivatives of sugars supported widely varying amounts of aflatoxin production. Amino acids and tricarboxylic acid cycle intermediates except aspartate and malate, respectively, did not appear to support de novo aflatoxin synthesis. Embden-Meyerhoff pathway intermediates prior to, but not after, 3-phosphoglycerate supported aflatoxin synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4565.1984.tb00488.x
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  • 5
    Electronic Resource
    Electronic Resource
    THE EFFECTS OF β-METHYLGLUCOSE, 3-0-METHYLGLUCOSE and THIOGLUCOSE ON AFLATOXIN PRODUCTION BY ASPERGILLUS PARASTICUS (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 8 (1986), S. 0 
    Details
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Earlier work characterizing the effects of glucose analogs on growth and aflatoxin production by Aspergillus parasiticus was expanded by assessing the effects of β-methylglucose (βMG), 3-0-methylglucose (3MG) and thioglucose (TG). As sole carbon source of conidia-initiated cultures, βMG and 3MG, but not TG, supported growth, but none supported toxin production. In glucose-containing replacement cultures, MG appeared to stimulate toxin production, while TG was inhibitory and 3MG had no effect. Preliminary assessment of the effects of βMG, 3MG, TG, 2-deoxyglucose and α-methylglucose on glucose uptake and utilization by glucose-containing replacement cultures indicated that under conditions that favor aflatoxin production, none of the analogs inhibited the uptake of 14C-labelled glucose. It appears that the glucose transport system(s) of A. parasiticus may be unusual in that it is insensitive to a variety of glucose analogs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4565.1986.tb00558.x
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of miconazole on growth and aflatoxin production by Aspergillus parasiticus (1987)
    Springer
    Mycopathologia 100 (1987), S. 135-144 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract At 5 μM, miconazole prevented the growth of Aspergillus parasiticus Speare in a number of media. Sensitivity to miconazole was increased approximately 10-fold in a medium containing glycerol. At sub-inhibitory concentrations, miconazole stimulated aflatoxin synthesis on media which normally support toxin formation. Miconazole inhibited respiration and altered mitochondrial ultrastructure, suggesting that miconazole inhibits growth and stimulates aflatoxin production by depressing mitochondrial activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437039
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    Paper (German National Licenses)
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