ISSN:
1432-2013
Keywords:
Pancreas
;
Bicarbonate Secretion
;
Buffer Secretion
;
H+ Ion Transport
;
Weak Organic Acids
;
Pankreas
;
Bicarbonatsekretion
;
Puffersekretion
;
H+-Ionentransport
;
schwache organische Säuren
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The isolated cat pancreas was perfused with a Krebs-Henseleitsolution containing different concentrations of sulfamerazine buffer. Concomitantly the secretory rate, pH value, Cl− and total buffer concentration in the secretion were measured. The following were found: 1. The secretory rate dropped to very small values when the bicarbonate buffer was omitted from the perfusate. 2. Sulfamerazine can partly replace the CO2-bicarbonate buffer in promoting water and solute secretion in the saline-perfused preparation of the cat's pancreas. 3. The secretion of the sulfamerazine buffer depended exclusively on the concentration of its undissociated component in the perfusate. It is concluded that a separation of H+/OH− ions takes place presumably at the luminal cell border and that the undissociated form of sulfamerazine can penetrate the cell by ‘nonionic diffusion’. In this way a source of H+ ions is provided which may be used either for H+ transport or for the buffering of OH− ions. The process of nonionic diffusion seems to be rate limiting for the buffer secretion, at least when sulfamerazine is offered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00586444
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