ISSN:
1573-5028
Keywords:
rpL20, L32, L33, L36, S12, S16, S19
;
formyl-methionine processing
;
RP-HPLC and HPEC
;
spinach (Spinacia oleracea)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Putative genes for 21 ribosomal proteins (RPs) have been identified in the chloroplast DNA of four plants by nucleotide sequencing and homology comparison but few of the gene products have been characterized. Here we report the purification and N-terminal sequencing of seven proteins from the spinach chloroplast ribosome. The data show them to be the homologues of Escherichia coli RPs L20, L32, L33, L36, S12, S16 and S19, and thus support the view that their genes identified in the chloroplast DNA represent functional genes. The initiating methionine residue was not detected in the mature protein in most cases but it was present in S16, indicating that only the formyl group is removed in this case. This result and the previously reported finding of N-methyl alanine at the N-terminus of chloroplast L2 indicate the existence of multiple N-terminal processing pathways in the chloroplast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040605
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