ISSN:
0006-3592
Keywords:
fiber optic biosensor
;
penicillin
;
penicillinase
;
immobilized enzyme
;
flow injection analysis
;
fluorescein isothiocyanate
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A penicillin fiber optic sensor is described. The sensor is based on co-immobilization of a pH indicator, fluorescein isothiocyanate (FITC), and penicillinase on a preactivated biodyne B membrane attached to the end of a bifurcated optical fiber. The characteristics of the sensor are investigated in conjunction with a flow injection analysis system. The proposed sensor is reversible and responds to penicillin in the concentration range of 1 × 10-4 to 5 × 10-2 mol/L. The application of this sensor to penicillin analysis in some pharmaceutical samples is demonstrated.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260391111
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