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  • 1
    Electronic Resource
    Electronic Resource
    E. coli expressed proteins as diagnostic reagents for typing of foot-and-mouth disease virus (1999)
    Springer
    Archives of virology 144 (1999), S. 1701-1712 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Truncated proteins corresponding to the C-terminal half of VP1 of four vaccine strains and two field variants of foot-and-mouth disease virus (FMDV) were expressed in E. coli. The expressed proteins were affinity purified and their type specific reactivity was confirmed by immunoprecipitation with anti-virus antibodies. Antibodies were raised against the purified proteins in guinea pigs and the type specificity of the anti peptide antibodies was confirmed by antigen capture reverse transcription polymerase chain reaction (Ag-RT/PCR) where the sera against a particular type captured the homologous virus. Antibodies were purified by immuno-affinity chromatography and tested for specificity by various serological tests. Using the purified proteins and the antibodies raised against them, tests like ELISA, Ag-RT/PCR, and latex agglutination test (LAT) were standardized. Application of the reagents in various tests was studied by screening a few field samples and by nucleotide sequencing. Specific reactivity of antibodies raised against expressed protein was seen with both vaccine virus and field samples. Thus E. coli expressed proteins and antibodies to them may form an alternative and cheap source of diagnostic reagents. The studies showed that antibodies against peptides were mono-specific and therefore may be used in LAT for rapid typing of FMDV and Ag-RT/PCR for typing ELISA negative field samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050698
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of foot-and-mouth disease virus antibody using counterimmunoelectrophoresis and serum neutralisation tests (1985)
    Springer
    Tropical animal health and production 17 (1985), S. 89-92 
    Details
    ISSN: 1573-7438
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Une enquête comparative a été réalisée sur l'application, la sensibilité et la spécificité de la contre-immunoélectrophorèse (CIEP) pour la détection rapide des anticorps du virus de la fièvre aphteuse dans les sérums de bovins en utilisant comme point de référence un test standard de neutralisation de sérum. On a conclu à la sensibilité et une spécificité raisonnable du test CIEP.
    Abstract: Resumen Se realizó una investigación conparativa sobre la aplicabilidad, sensibilidad y especificidad de la prueba de contrainmunoelectroforesis (CIEF), para la detección rápida de anticuerpos del virus de la fiebre aftosa en suero bovino, teniendo como referencia la prueba estandard de seroneutralización. La prueba de CIEF fue sensitiva, exhibiendo cierto grado de especificidad.
    Notes: Summary A comparative investigation was made on the applicability, sensitivity and specificity of counterimmunoelectrophoresis (CIEP) for the rapid detection of antibody to foot-and-mouth disease virus in cattle sera using as reference a standard serum neutralisation test. The CIEP test was sensitive and exhibited a reasonable specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02360779
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Hexokinase activity as marker to assess time of harvest of foot-and-mouth disease virus in BHK21 Cl13 cell cultures (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 613-615 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hexokinase (B.C. 2.7.1.1) activity as a marker enzyme during FMD viral infection has been observed spectrophotometrically in a system coupled with glucose-6-phosphate dehydrogenase, in supernatants of BHK21Cl13 suspension as well as anchored cell culture at a minimum of 104 infective virus particles/ml. Specific activity increased with virus concentration in culture supernatants and abruptly decreased with a fall in virus titer, as has been noted by TCID/50,146 S concentration, and enzyme-linked immunosorbent assay (ELISA) readings.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260280420
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    Paper (German National Licenses)
    Fulltext
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