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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of environmental samples for organochlorine insecticides and related compounds by high-resolution electron capture gas chromatography with glass capillary columns (1977)
    s.l. : American Chemical Society
    Environmental science & technology 11 (1977), S. 1109-1113 
    Details
    ISSN: 1520-5851
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/es60135a001
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The MT3 specificity resides on a novel human class II antigen distinct from the HLA-DR antigen and DC-like antigen (1984)
    Springer
    Immunogenetics 20 (1984), S. 155-167 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The MT3 specificity is closely associated with the HLA-DR4, DR7, and DRw9, and is a supertypic specificity. To determine whether the MT3 specificity resides on a novel class II antigen, the MT3 antigen, DR antigen and the DC-like antigen from the DR4-, DR7- and DRw9-homozygous B lymphoid cell lines were identified and compared with one another by two-dimensional gel electrophoresis using alloantisera. The analysis revealed that each of the three antigens exists as a structurally distinct class II antigen in each cell line. The light chains of the MT3, DR and DC-like antigens are different in charge from one another. The molecular weight of the heavy chains of the MT3 and DR antigens is higher than that of the DC-like antigen. On the other hand, no electrophoretic differences are observed between the heavy chains of the MT3 and DR antigens. These results strongly suggest that the MT3 specificity resides on a light chain of a novel class II antigen distinct from the DR antigen and the DC-like antigen. These observations also support our previous proposition that the MT3 antigen belongs to the fourth group of the human class II antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364487
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of the MT3 molecule using two-dimensional gel electrophoresis and alloantisera (1983)
    Springer
    Immunogenetics 18 (1983), S. 575-583 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The MT3 antigen is defined serologically as a DR supertypic specificity and is strongly associated with DR4, DR7, and DRw9. To determine whether the MT3 molecule is distinct from the DR molecule, DR4 and MT3 antigens were immunoprecipitated from 125I-labeled plasma membrane glycoproteins of a DR4-homozygous, MT3-homozygous B lymphoid cell line, Wa, and compared by two-dimensional (2-D) gel electrophoresis. The precipitates with two different anti-DR4 alloantisera and with three different mouse antibodies against human Ia monomorphic determinants gave the same 2-D gel pattern consisting of one heavy chain with a molecular weight of 34 000 and a set of light chains with a molecular weight of 30000, indicating that these polypeptides are the components of the DR4 molecule. On the other hand, all three anti-MT3 alloantisera used precipitated an identical set of anti-MT3 alloantisera specific light chains with a molecular weight of 30 000, and one heavy chain with a molecular weight of 34 000. The pl of the MT3 light chain was more acidic than that of the DR4 light chain. The amount of MT3 light chains was much smaller than that of DR4 light chains in unlabeled plasma membrane glycoproteins. Thus, we have demonstrated directly using 2-D gel electrophoresis and anti-MT3 alloantisera that the MT3 antigen is a new human Ia molecule distinct from DR4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00345965
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  • 4
    Electronic Resource
    Electronic Resource
    Curative effects of combination therapy with lentinan and interleukin-2 against established murine tumors, and the role of CD8-positive T cells (1994)
    Springer
    Cancer immunology immunotherapy 38 (1994), S. 1-8 
    Details
    ISSN: 1432-0851
    Keywords: IL-2 ; Lentinan ; Synergistic antitumor effects ; Tumor-specific CTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The antitumor activity of a combination of an antitumor polysaccharide, lentinan (a β1–3 glucan with β1–6 branches), and interleukin-2 (IL-2) was evaluated against established MBL-2 lymphoma and S908.D2 sarcoma at i.d. sites. Treatment of the MBL-2-tumor-bearing BDF1 mice with lentinan and IL-2 induced complete regression of tumor in 87.5% of mice treated. In contrast, treatments using either lentinan or IL-2 alone failed to induce complete regression of tumor, although temporal growth inhibition of tumor was observed about in half of the mice treated. Improvements of antitumor effects by the combination of lentinan and IL-2 were also observed in the MBL-2/B6 and S908.D2/B10.D2 systems. Expression of the antitumor effects of lentinan/IL-2 treatments required the intact T cell compartment, because the effects were not observed when nude mice were used. In the MBL-2/B6 system, the antitumor action of lentinan/IL-2 treatment was abolished in mice treated with antibody to CD8 antigen, whereas antibodies to CD4 or NK1.1 were ineffective. Furthermore, augmented tumor-specific cytotoxic T lymphocyte (CTL) activity was observed in regional lymph node cells of the mice after lentinan and IL-2 administration. These data indicate that the antitumor effects of lentinan/IL-2 are mediated by CD8+ CTL but not by CD4+ T cells or NK1.1+ NK/LAK cells, and suggest that this combined therapy may be effective against even established tumors that are resistant to IL-2 therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01517163
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  • 5
    Electronic Resource
    Electronic Resource
    Curative effects of combination therapy with lentinan and interleukin-2 against established murine tumors, and the role of CD8-positive T cells (1994)
    Springer
    Cancer immunology immunotherapy 38 (1994), S. 1-8 
    Details
    ISSN: 1432-0851
    Keywords: Key words: IL-2 – Lentinan – Synergistic antitumor effects – Tumor-specific CTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The antitumor activity of a combination of an antitumor polysaccharide, lentinan (a β1-3 glucan with β1-6 branches), and interleukin-2 (IL-2) was evaluated against established MBL-2 lymphoma and S908.D2 sarcoma at i. d. sites. Treatment of the MBL-2-tumor-bearing BDF1 mice with lentinan and IL-2 induced complete regression of tumor in 87.5% of mice treated. In contrast, treatments using either lentinan or IL-2 alone failed to induce complete regression of tumor, although temporal growth inhibition of tumor was observed about in half of the mice treated. Improvements of antitumor effects by the combination of lentinan and IL-2 were also observed in the MBL-2/B6 and S908.D2/B10.D2 systems. Expression of the antitumor effects of lentinan/IL-2 treatments required the intact T cell compartment, because the effects were not observed when nude mice were used. In the MBL-2/B6 system, the antitumor action of lentinan/IL-2 treatment was abolished in mice treated with antibody to CD8 antigen, whereas antibodies to CD4 or NK1.1 were ineffective. Furthermore, augmented tumor-specific cytotoxic T lymphocyte (CTL) activity was observed in regional lymph node cells of the mice after lentinan and IL-2 administration. These data indicate that the antitumor effects of lentinan/IL-2 are mediated by CD8+ CTL but not by CD4+ T cells or NK1.1+ NK/LAK cells, and suggest that this combined therapy may be effective against even established tumors that are resistant to IL-2 therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01517163
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Multiple organochlorine pesticide residues in Japan (1973)
    Springer
    Bulletin of environmental contamination and toxicology 10 (1973), S. 145-150 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01720694
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  • 7
    Electronic Resource
    Electronic Resource
    Photodieldrin residues in field soils (1974)
    Springer
    Bulletin of environmental contamination and toxicology 12 (1974), S. 275-280 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01709119
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  • 8
    Electronic Resource
    Electronic Resource
    Persistence of BHC in river water in the Kitakyushu District, Japan, 1970–1974 (1975)
    Springer
    Bulletin of environmental contamination and toxicology 14 (1975), S. 380-384 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01685654
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Structures of chlorosomes and aggregated BChlc inChlorobium tepidum from solid state high resolution CP/MAS13C NMR (1994)
    Springer
    Photosynthesis research 41 (1994), S. 211-223 
    Details
    ISSN: 1573-5079
    Keywords: antenna structure ; green photosynthetic bacteria ; cross polarization ; magic angle spinning ; ring current shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cross polarization/magic angle spinning (CP/MAS)13C (solid state high resolution) NMR spectra were observed for chlorosomes and BChlc aggregates. Similarity of both kinds of spectra (except for some signals assignable to proteins and lipids in chlorosomes) indicates that BChlc's in chlorosomes are present just as in synthetic BChlc aggregates. Chemical shifts for C131 carbonyl and C31 hydroxylethyl carbons indicate hydrogen bonding between them. Comparison of solution and solid state13C NMR chemical shifts shows the five coordinated nature of BChlc aggregates. Some chemical shift differences were attributable to ring currents shifts. Their comparisons with calculated ring current shift values predicted structures for the aggregates. Cross polarization dynamics of the CP/MAS13C NMR signals explored dynamic and structural nature of the BChlc aggregates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184162
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of the HLA-DRw9 antigen using two-dimensional gel electrophoresis and alloantisera (1984)
    Springer
    Journal of human genetics 29 (1984), S. 17-25 
    Details
    ISSN: 1435-232X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary HLA-DRw9 antigen, of which frequency is relatively high in Japanese, is identified and analyzed by two-dimensional gel electrophoresis using anti-DRw9 alloantisera and monoclonal antibodies against human class II antigens. The DRw9 antigen consists of one set of heavy and light chains on the cell surface and the Ii chain is associated with the heavy chain-light chain complex in intracellular regions. The DRw9 light chain is distinct from the DR4 and DR7 light chains but the DRw9 heavy chain is identical with the DR4 and DR7 heavy chain, suggesting that the DRw9 light chain carries the antigenic specificity recognized by the anti-DRw9 alloantisera. The amount of the DRw9 antigen is extremely large compared with the other class II antigens in the total class II antigen pool, as reported previously for the DR4 antigen. The data presented in this paper indicate that the DRw9 antigen is present as an independent DR molecule and has the polypeptide constitution characteristic of the DR antigen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01876753
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