ISSN:
1432-1017
Keywords:
Key words Arginine-vasopressin analogues
;
Energy transfer
;
Fluorescence quenching
;
Acrylamide
;
Molecular mechanics
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Analogues of arginine-vasopressin (AVP) in which substitution of the proline residue in position 7 (by either sarcosine or N-methylalanine) combined with replacement of the cysteine residue in position 1 were the subject of a fluorescence and molecular mechanics study. We obtained two groups of analogues: selective antidiuretic agonists (cysteine or β-mercaptopropionic acid in position 1) and pressor and uterotonic antagonists (deaminopenicillamine or β-mercapto-β,β-cyclopentamethylene- propionic acid in position 1). Using frequency-domain measurements of fluorescence resonance energy transfer (FRET) we estimated the distance distribution between the phenolic ring of Tyr2 and the disulphide bridge Cys1–Cys6. We also analyzed acrylamide quenching of tyrosyl fluorescence to determine the exposure of the tyrosyl ring to the solvent. Results from fluorescence experiments were compared with those from Monte Carlo simulation (ECEPP/3 force-field).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002490050070
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