ISSN:
0952-3499
Keywords:
capillary electrophoresis
;
laser-induced fluorescence
;
polynuclear aromatic hydrocarbons
;
cyclodextrins
;
inclusion complexes
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Two neutral cyclodextrins (CDs), hydroxypropyl-β-CD (HPβOD) and methyl-β-CD (MβCD), and two negatively charged forms, carboxymethyl-β-CD (CMβCD) and sulfobutylether-β-CD (SBβCD), were used to demonstrate the principle of separation of hydrophobic and non-charged polynuclear aromatic hydrocarbons (PAHs) by capillary electrophoresis (CE) based on differential partitioning of analytes between the CDs. The estimation of the partition coefficient (Ki) confirmed that the PAH components which partitioned most to the negative cyclodextrin (i.e. with the highest Ki) exhibited the longest migration time. The Ki values obtained for the CMβCD-HPβCD system are all below 1, indicating that the PAHs prefer to remain in the neutral HPβCD. The Ki values for the SBβCD-MβCD buffer are all greater than 1, and cover a wider range, indicating preference of the PAHs for the anionic derivative. Methods were applied for separation of a PAH mixture containing various important PAHs on the US Environmental Protection Agency priority list. This novel procedure provided much better separation of PAH isomers, including benzo[a]pyrene and benzo[e]pyrene, than has been reported previously using CE. The system was applied to the sensitive determination of nanomolar levels of PAHs in contaminated soil.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
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