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  • 1
    Electronic Resource
    Electronic Resource
    Paralytic poliomyelitis in a child with agammaglobulinemia (1979)
    Springer
    European journal of pediatrics 132 (1979), S. 11-16 
    Details
    ISSN: 1432-1076
    Keywords: Poliomyelitis ; Agammaglobulinemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This paper gives the clinical, immunological and virological data on a patient with agammaglobulinemia who developed paralytic poliomyelitis. The patient was a 3 year-old boy who had a typical B-cell defect without a T-cell defect. He had profound hypogammaglobulinemia and defective plasma cells and had repeated pyogenic infections which were controlled by gammaglobulin replacement therapy. At 3 years of age, he was admitted to our hospital with suspected meningitis. He had fever, tremor and neck stiffness for 3 days and subsequently developed paralysis in his left arm and right leg. There was lymphocytosis in the cerebrospinal fluid. A non vaccine-like strain of poliovirus type 2 was isolated from the stool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00443199
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  • 2
    Electronic Resource
    Electronic Resource
    Rescue of host-dependent conditional lethal mutants of vaccinia and rabbitpox viruses by Yaba virus (1977)
    Springer
    Archives of virology 55 (1977), S. 341-345 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Host-dependent conditional lethal mutants of vaccinia and rabbitpox viruses were rescued in nonpermissive host cells by Yaba virus, a poxvirus serologically distant from the rescued viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315056
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Variation in susceptibility of HeLa cell lines to coxsackievirus A 9 (1979)
    Springer
    Archives of virology 59 (1979), S. 213-222 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the course of serial passages for several years a line of uncloned HeLa cells (A line) showed a gradual decrease in plaquing efficiency by coxsackievirus A 9 (CA 9 virus), while subcultures prepared from the same line kept frozen at an early passage level (A original line) did not show any change. However, it was observed later that the plaque-forming ability of the A original line (A orig. line) also decreased after serial passages as was observed with the A line. Comparing the characteristics of the same cell line at two different passage levels, it was found that the efficiency of adsorption of virus to cells was nearly the same, while virus yield at 8 hours after infection was different. The activity of alkaline phosphatase of cells was also different between these two passage levels, suggesting that a high enzymatic activity is associated with the susceptibility of cell cultures to CA 9 virus. Magnesium chloride at 25 mM enhanced plaque formation by CA 9 virus in highly passaged less susceptible cell cultures, and a possible role of the chemical as a stabilizer of alkaline phosphatase was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317416
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  • 4
    Electronic Resource
    Electronic Resource
    Comparative studies of several vaccinia virus strains by intrathalamic inoculation into cynomolgus monkeys (1977)
    Springer
    Archives of virology 53 (1977), S. 197-208 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary From the comparative studies of the virulence of several vaccinia virus strains by intrathalamic inoculation into cynomolgus monkeys, the following results were observed. The CV1 virus was most virulent, the New York City Board of Health, Ikeda, EM63, and Lister viruses were slightly less virulent, and DIs and LC 16 viruses least virulent. The characteristic findings were widespread inflammatory lesions in the meninges and choroid plexus which were closely associated with the replication of vaccinia virus, and parenchymal lesions which might be referred to as encephalopathy in the deceased monkeys. Meningoencephalitis was, however, often recognized in the monkeys sacrificed at 14 days postinoculation and those dying late.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314664
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  • 5
    Electronic Resource
    Electronic Resource
    Virion polypeptides of poxviruses (1980)
    Springer
    Archives of virology 63 (1980), S. 209-225 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Structural polypeptides from a number of poxviruses were analysed by SDS-polyacrylamide gel electrophoresis. The patterns obtained with orthopoxviruses were generally quite similar to one another, but variola, monkeypox, cowpox and vaccinia viruses could be distinguished by their profiles in the molecular weight (mol. wt.) region around 30,000 to 40,000; some additional variation was found amongst cowpox and vaccinia strains. Whitepox virus was shown to have structural polypeptides indistinguishable from those of variola virus. The structural polypeptides of poxviruses belonging to other genera were different from those of the orthopoxvirus, except those of mol. wt. about 122,000 and 97,000, which were common to all viruses irrespective of genus. A polypeptide of mol. wt. about 25,000 was also observed in all cases, though its position varied slightly with the individual virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315028
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  • 6
    Electronic Resource
    Electronic Resource
    Plaque assay of variola virus in a cynomolgus monkey kidney cell line (1970)
    Springer
    Archives of virology 32 (1970), S. 73-81 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Variola virus produced plaques in a cynomolgus monkey kidney cell line, JINET, under agar overlay medium. The plaque morphology of variola virus in these cells was clearly distinct from that of other members of the variola-vaccinia subgroup of poxvirus and the plaque morphology marker of variola virus may be used for the identification of variola virus. The plaque number of variola virus was enhanced by the addition of DEAE-dextran and MgCl2 to agar overlay medium at concentrations of 100 to 200 Μg/ml and 15 to 45 mm, respectively. The sensitivity of the plaque method in the presence of 100 Μg/ml of DEAE-dextran and 25 mm MgCl2 in the overlay medium was equal to that of the usual CAM method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241522
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  • 7
    Electronic Resource
    Electronic Resource
    Strain-specific aggregation of enterovirus by dextran sulfate (1981)
    Springer
    Archives of virology 70 (1981), S. 123-135 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Dextran sulfate aggregates several enteroviruses depending not only on the pH, the ionic strength of the medium, but also on the protein content of the fluids and on strain specificities of the viruses. The aggregation effect was measured by filtration experiments, by sedimentation in the ultracentrifuge and by electron microscopy. The well known inhibiting effect of dextran sulfate on plaque formation may be due to its aggregating effect: A very strong inhibition of the release of maturated virions from the infected cells is observed in medium containing dextran sulfate, whereas the adsorption process is inhibited much less compared with PBS controls. The maximal effect on virus aggregation, plaque size and virus release is observed at the same concentration of dextran sulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315006
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