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Notizen: Bone marrow cells have been used to potentiate wound healing in chronic wounds (Badiavas and Falanga, 2003). We postulate that the performance of these autografted cells may be enhanced by seeding them into scaffolding with normal skin architecture; indeed that the differentiation of the precursor cells may be determined by the juxtaposition of intact extracellular matrix. This might in turn lead to a prefabricated skin substitute capable of recapitulating fully functional skin.We are seeding murine gfp+ bone marrow cells, containing mixed mesenchymal and hematopoietic precursor cells into Alloderm®, a decellularized preserved skin graft. This seeding is potentiated by meshing the skin and gentle centrifugation of fresh marrow into the dermal aspect of the allograft. Other samples were merely co-cultured with the cells in Dexter’s media. Duplicate samples were fixed and stained with DAPI at 1, 3, 7, and 14 days. Viewed under fluorescence, gfp+ cells are bright green.Comparing these images with DAPI-staining allows viable cells to be identified which are gfp+. H&E micrographs were used to ascertain cellular morphology. Results: Scant cells were observed in the allografts at the early time points. The cells were pleiomorphic with large nuclei, resembling hematopoietic precursors(〈link href="#fu1"〉Fig 1). Later, at days 7 and 14, more abundant cells were apparent, including some in the interior of the dermis. This signifies either migration or seeding via centrifugation. The morphology of these cells is significantly different; the spindle shape suggests a fibroblast (〈link href="#fu2"〉Fig 2). In addition, the population of gfp+ cells has markedly expanded by day 14, attaining near confluence in some sections (〈link href="#fu3"〉Fig 3). No cells were seen in controls (〈link href="#fu4"〉Fig 4). Conclusion: True skin replacement will only be possible when the panoply of skin appendages can be recapitulated. The experiments presented here are evidence that pluripotential precursor cells can be seeded into dermal matrices providing an optimal environment for regeneration.〈figure xml:id="fu1"〉1〈mediaResource alt="image" href="urn:x-wiley:10671927:WRRABSTRACTEP:image_n/WRR_abstractep_fu1.eps"/〉〈figure xml:id="fu2"〉2〈mediaResource alt="image" href="urn:x-wiley:10671927:WRRABSTRACTEP:image_n/WRR_abstractep_fu2.eps"/〉〈figure xml:id="fu3"〉3〈mediaResource alt="image" href="urn:x-wiley:10671927:WRRABSTRACTEP:image_n/WRR_abstractep_fu3.eps"/〉〈figure xml:id="fu4"〉4〈mediaResource alt="image" href="urn:x-wiley:10671927:WRRABSTRACTEP:image_n/WRR_abstractep_fu4.eps"/〉