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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 72 (1970), S. 60-67 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Konzentration an Acetaldehyd im Medium während der anaeroben Vergärung von Glucose durch Saccharomyces cerevisiae weist in der logarithmischen Wachstumsphase die höchsten Werte auf. Die Induktion der Pyruvatdecarboxylase durch Glucose fördert die Akkumulation von Acetaldehyd, der ins Medium diffundiert. Hefestämme, die unterschiedlich viel Acetaldehyd bilden, unterscheiden sich in ihren Pyruvatdecarboxylaseaktivitäten. Diese engen Beziehungen zwischen Pyruvatdecarboxylase und Acetaldehydproduktion deuten auf die Kontrollfunktion der Pyruvatdecarboxylase bei der Acetaldehydakkumulation hin. Höhere Aktivitäten der Alkoholdehydrogenase verringern die Acetaldehydakkumulation, wodurch sich ein Hinweis auf die Rolle dieses Enzyms bei der Regulation der Acetaldehydkonzentration ergibt.
    Notes: Summary During fermentation of glucose by Saccharomyces cerevisiae maximum acetaldehyde production coincides with maximum pyruvate decarboxylase activity in the logarithmic phase of growth. The stimulation of this enzyme by high glucose levels leads to an increased formation of acetaldehyde, which diffuses into the medium. Yeast strains, which produce varying amounts of acetaldehyde also exhibited varying pyruvate decarboxylase activities. The close relationship between pyruvate decarboxylase activity and acetaldehyde production suggests a control function of the enzyme in acetaldehyde accumulation. Apart from pyruvate decarboxylase higher activities of alcohol dehydrogenase show the reversed influence on aldehyde concentration, thus demonstrating the role of alcohol dehydrogenase in aldehyde production.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Während der aeroben Vergärung von Glucose wurde die Konzentration von Acetaldehyd im Gärmedium über den gesamten Gärablauf bei mehreren Stämmen von Saccharomyces cerevisiae verfolgt. Die Aldehydkonzentration weist bei Glucosekonzentrationen zwischen 5 und 20% zwei Maxima auf. Damit ist der Konzentrationsverlauf von Acetaldehyd aerob wesentlich anders als bei der anaeroben Gärung, mit nur einem meist niedrigen Maximum. 10-3 M Azid hemmt die Bildung von Acetaldehyd ganz oder weitgehend. Das deutet auf die Funktion bzw. Synthese der Cytochrome, die in Gegenwart von Sauerstoff offensichtlich auch bei hohen Glucosekonzentrationen nicht vollständig reprimiert werden. Der durch die Atmung bedingte Wasserstoffabfluß führt zu höheren Aldehydkonzentrationen. Der in der logarithmischen Wachstumsphase vorwiegend fermentative Stoffwechsel überlagert mit seiner starken Wasserstoffproduktion die Atmung, was zum Auftreten von zwei Aldehydmaxima führt. Die Regulation der Acetaldehydbildung während der aerohen Gärung wird eingehend diskutiert und zeigt, daß Acetaldehyd als Indicator für die Induktion und Funktion der Atmungsenzyme geeignet ist.
    Notes: Summary During fermentation of glucose by the yeast Saccharomyces cerevisiae small amounts of acetaldehyde are formed. Anaerobically, acetaldehyde accumulates in the medium, showing only one maximum of ca. 10–30 mg/l in the logarithmic growth phase. During aerobic fermentation, acetaldehyde is formed in higher amounts (160 mg/l) and two maxima are observed. Both maxima appear in glucose concentrations varying from 5–20%. The addition of azide, which inhibits respiration results in a loss of acetaldehyde production. Therefore it is assumed, that the enzymes of the respiratory chain are involved in the formation of acetaldehyde and that acetaldehyde production is caused by induction and function of cytochromes under the influence of oxygen. Various yeast strains differ in their ability of acetaldehyde production. The characteristic appearance of two aldehyde maxima is explained by exceeding hydrogen production in the logarithmic phase of growth, where the fermentation suppresses the influence of respiration on aldehyde production. The regulation of the formation of acetaldehyde during aerobic fermentation is thoroughly discussed showing that acetaldehyde can serve as an indicator for the activity of respiration enzymes in yeast.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    BBA Section Nucleic Acids And Protein Synthesis 287 (1972), S. 98-105 
    ISSN: 0005-2787
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 138 (1968), S. 163-169 
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zusammenfassung Auf dem Prinzip der Mikrodiffusion unter Verwendung von 3-Methyl-2-benzothiazolon-hydrazon als Reagens wird eine Methode erarbeitet, die zur raschen und genauen Bestimmung von Acetaldehyd (0,5–20μg/ml) in biologischen Flüssigkeiten einschließlich Wein geeignet ist. Eine Reihe von möglichen Störfaktoren bei der Anwendung wird überprüft.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 28 (1972), S. 1118-1119 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary EDTA, which is reported to potentiate the action of some antibiotics and chemotherapeutics in various bacterial strains, showed only additive effects inE. coli, when combined with trimethoprim or sulfamethoxazole, eithersingly or together. Neither was a potentiation seen with sulfanilamide, the permeation and effectiveness of which is less pronounced than that of sulfamethoxazole. The same negative results were obtained with levallorphan, which leads to alterations in the cell membrane. EDTA may therefore be of chemotherapeutic interest only in special cases, e.g. inPsuedomonas species which possess a highly EDTA-sensitive cell wall.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 6 (1987), S. 451-455 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The beta-lactamase inducing properties of various new beta-lactam antibiotics in two isogenic strains ofEnterobacter cloacae were investigated. Beta-lactamase activity was measured two hours after addition of inducer to cells in the late logarithmic growth-phase. Beta-lactamase expression was highly dependent on the growth medium used, highest levels being obtained after induction with cefoxitin in Tryptic Soy broth, Mueller-Hinton broth and Nutrient broth. Upon induction the mutant 908 Ssi produced tenfold higher beta-lactamase levels than its parent wild type 908 Swi. Among the new antibiotics investigated, sulfoxides of several oxyimino-cephalosporins, HR 810, cefetamet, cefteram, carumonam and BRL 36650 were moderate or poor inducers. The penem FCE 22101 resembled imipenem in its strong inducing properties.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 8 (1989), S. 536-543 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The in vitro activity of cefetamet, the microbiologically active metabolite of the orally administered prodrug cefetamet pivoxil, was compared with that of cephalexin, cefaclor, cefuroxime and amoxicillin. Cefetamet was highly active againstEnterobacteriaceae, Neisseria spp.,Vibrio spp.,Haemophilus influenzae and streptococci other than enterococci. Cefetamet inhibited cefaclor-resistant species such asProteus vulgaris, Providencia stuartii, Providencia rettgeri andSerratia marcescens. Staphylococci,Pseudomonas aeruginosa and cephalosporinase-overproducing strains ofEnterobacter cloacae were resistant to cefetamet. The superior activity of cefetamet compared with older oral beta-lactam antibiotics against a large number of gram-negative pathogens correlated with enhanced stability towards betalactamases. In accordance with the in vitro findings, cefetamet pivoxil showed good activity in experimental infections in the mouse and rat, suggesting satisfactory bioavailability in these animals after oral administration.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The new bacteriocidal drug epiroprim (Ro-11-8958) was tested (alone or in combination with dapsone) on its efficacy againstToxoplasma gondii andPneumocystis carinii in their hosts: laboratory mice and/or nude mice/rats, and was compared to the curative effects of the recent drugs of choice. The experiments clearly pointed out that epiroprim has significant effects on the reduction of both parasites when given alone. In combination with dapsone epiroprim led to a complete cure of toxoplasmosis in mice. This finding is of some importance for AIDS patients mostly suffering from bacteriosis and parasitosis at the same time.
    Type of Medium: Electronic Resource
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