ISSN:
1432-1017
Keywords:
Nucleoprotein structure
;
small-angle X-ray scattering
;
light-scattering
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Structural parameters of phage T7 were compared in two frequently use Tris buffers of high and low ionic strength, in order to explain the different biological activity and drug-binding characteristics. Characteristics of the whole phage geometry were obtained by viscosimetry, static and quasi-elastic light-scattering and small-angle X-ray scattering. The latter method revealed dissimilarities in the intraphage DNA compactness, consistent with the findings of the optical absorption melting studies. Alterations in the particle dimensions determined in the same sample by different methods are discussed, and a model is constructed to explain the structural modifications that occur on lowering the ionic strength.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00256480
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