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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Microbial ecology 3 (1976), S. 1-9 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Enrichment cultures were prepared with different media for phototrophic bacteria from four species of marine sponges, collected from oxic coastal waters near Split (Yugoslavia). We obtained pure cultures of six strains ofChromatiaceae and two strains ofRhodospirillaceae by agar shake dilution. TheRhodospirillaceae were identified asRhodopseudomonas sulfidophila and a marine form ofRhodopseudomonas palustris. TheChromatiaceae were identified asChromatium vinosum, Chromatium gracile, Chromatium minutissimum. Ectothiorhodospira mobilis, and a Chromatium species, which in some respects resemblesChromatium minus. The occurrence of strictly anaerobic phototrophic bacteria in aerobic sponges is discussed with respect to nutrition and possible syntrophism.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 107 (1976), S. 289-292 
    ISSN: 1432-072X
    Keywords: Wine yeasts ; Sulfur metabolism ; Sulfite formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eight different strains of wine yeasts were investigated phenomenologically with respect to their fermentation characteristics and sulfite production. It was established that the amount of produced sulfite is specific for each strain and proceeds parallel to the fermentation of sugar. A reduced production of biomass observed in the strongly sulfite-forming strains might be a result of an intracellular inhibition by the produced sulfite.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 108 (1976), S. 99-104 
    ISSN: 1432-072X
    Keywords: Wine yeasts ; Sulfur metabolism ; Regulation ; Sulfite reductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The enzyme catalyzing the reduction of sulfite by reduced benzyl viologen (BVH) was partially purified and characterized from two strains of wine yeasts, a sulfite-producing strain and a non-producing strain. Both enzymes showed corresponding features in pH-optima, optima of buffer and benzyl viologen concentrations. The enzymes did not catalyze the reduction of nitrite by reduced viologen dyes, but the reduction of sulfite was uncompetitively inhibited by nitrite. Compounds of sulfur metabolism such as sulfate, thiosulfate, cysteine, serine and methionine did not influence the activity of either of the enzymes. The main differences between the two enzymes exist in the specific activities in crude extracts, the K m -values for sulfite, substrate inhibition rates, and localization in different fractions during (NH4)2SO4 precipitation. The specific activity in crude extracts of the sulfite-producing strain (0.052 μmoles S2- x min-1 x mg-1) was about three fold higher than that of the non-producing strain (0.0179 μmoles S2- x min-1 x mg-1). On the other hand the sulfite-producing strain had a higher K m -value for sulfite (2×10-3 M) and was more strongly inhibited by the substrate than the non-producing strain (6×10-3 M).
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 118 (1978), S. 243-247 
    ISSN: 1432-072X
    Keywords: Wine yeasts ; Sulfur metabolism ; Sulfite formation ; ATP-sulfurylase ; ADP-sulfurylase ; Selenite reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four strains of Saccharomyces cerevisiae and Saccharomyces bayanus differing in their ability to produce sulfite from sulfate were studied with respect to their in vivo regulation of adenosine 5′-triphosphate-sulfurylase and adenosine 5′-diphosphate sulfurylase by various sulfur compounds and by selenite. High sulfite-producing strains showed a modified repression of ATP-sulfurylase as compared with low sulfite-producing strains. ADP-sulfurylase did neither show regulation by sulfurintermediates in high nor in low sulfite-producing strains. A stoichiometrical reduction of selenite to elemental selenium was found in the presence of sulfate or L-methionine as sole sulfur source.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 118 (1978), S. 249-251 
    ISSN: 1432-072X
    Keywords: Wine yeasts ; Sulfite formation ; Sulfur metabolism ; Sulfite reductase regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four strains of wine yeasts of two different species (Saccharomyces cerevisiae var. ellipsoideus and S. bayanus) were investigated with respect to regulation of NADPH- and benzyl viologen-dependent sulfite reductases by various sulfur sources. The enzyme activity was followed over a growth period of 96 h. The low sulfite-producing strains showed an increased biosynthesis of NADPH-dependent sulfite reductase during the exponential growth phase in the presence of sulfate, sulfite and djencolic-acid. This increase was not observed in the high sulfite-producing strains. Methionine and cysteine prevented this derepression. At the end of the exponential growth phase, enzyme biosynthesis was repressed again, presumably by sulfur-containing amino acids which were produced during growth. The regulatory influence of the various sulfur sources on benzyl viologen dependent sulfitereductase activity is obviously much weaker.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 124 (1980), S. 205-210 
    ISSN: 1432-072X
    Keywords: Thiobacillus denitrificans ; Sulfur metabolism ; Thiosulfate ; Elemental sulfur ; Siroheme sulfite reductase ; Rhodanese
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thiobacillus denitrificans strain RT could be grown anaerobically in batch culture on thiosulfate but not on other reduced sulfur compounds like sulfide, elemental sulfur, thiocyanate, polythionates or sulfite. During growth on thiosulfate the assimilated cell sulfur was derived totally from the outer or sulfane sulfur. Thiosulfate oxidation started with a rhodanese type cleavage between sulfane and sulfone sulfur leading to elemental sulfur and sulfite. As long as thiosulfate was present elemental sulfur was transiently accumulated within the cells in a form that could be shown to be more reactive than elemental sulfur present in a hydrophilic sulfur sol, however, less reactive than sulfane sulfur of polythionates or organic and inorganic polysulfides. When thiosulfate had been completely consumed, intracellular elemental sulfur was rapidly oxidized to sulfate with a specific rate of 45 natom S°/min·mg protein. Extracellularly offered elemental sulfur was not oxidized under anaerobic conditions.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-072X
    Keywords: Phototrophic bacteria ; Ectothiorhodospira vacuolata ; Gas vacuoles ; Alkaliphilic bacteria ; Halophilic bacteria ; Anaerobic sulfide oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new phototrophic bacterium was isolated from Jordanian and Kenyan alkaline salt lakes. Cells are rod shaped, 1.5 μm wide and 2–4 μm long, and motile by polar flagella. They divide by binary fission, and possess photosynthetic membranes as lamellar stacks similar to those in the other species of the genus Ectothiorhodospira and the brown colored Rhodospirillum species. The presence of bacteriochlorophyll a and carotenoids of the normal spirilloxanthin series is indicated by the absorption spectra of living cells. Under certain growth conditions the cells form gas vacuoles, may become immotile and float to the top of the culture medium. Sulfide and thiosulfate are used as photosynthetic electron donors. During the oxidation of sulfide to sulfate, elemental sulfur is formed, which is accumulated outside the cells. The organisms are strictly anaerobic, do not require vitamins, are moderately halophilic and need alkaline pH-values for growth. The new species Ectothiorhodospira vacuolata is proposed.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 135 (1983), S. 254-258 
    ISSN: 1432-072X
    Keywords: Ectothiorhodospira abdelmalekii ; Sulfide oxidation ; Cytochrome c-551 ; Sulfide: acceptor oxidoreductase ; Polysulfide ; Elemental sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cytochrome c-551 was isolated and purified from Ectothiorhodospira abdelmalekii. It is a small acidic haemoprotein with a molecular weight of 9,500, an isoelectric point of 3.5 and a redox potential of-7 mV at pH 7.0. It showed three maxima in the reduced state (α=551, β=529, γ=417). The best purity index (A280/A417) obtained was 0.29. During sulfide oxidation to elemental sulfur a considerable amount of polysulfides were transiently accumulated. The digestion experiment can be taken to indicate that cytochrome c-551 is localized on the outside of the cell membrane. The addition of cytochrome c-551 to a suspension of spheroplasts stimulated the velocity of sulfide oxidation. These experiments support the interpretation that cytochrome c-551 may be a sulfide: acceptor oxidoreductase.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 137 (1984), S. 145-150 
    ISSN: 1432-072X
    Keywords: Sulfate-reducing bacteria ; Sulfur metabolism ; Adenylylsulfate reductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four recently described species of new genera of sulfate-reducing bacteria, Desulfobulbus propionicus, Desulfobacter postgatei, Desulfococcus multivorans and Desulfosarcina variabilis were examined with respect to adenylylsulfate reductase. All of the species examined contained the enzyme in sufficient concentrations to account for dissimilatory sulfate reduction. Adenylylsulfate reductase was enriched 17.1-fold from Desulfobulbus propionicus by ammonium sulfate fractionation, ion exchange chromatography and gel filtration. The molecular weight was 175,000 and the enzyme contained 1 mol of flavin, 8 mol of non heme iron and 8 mol of labile sulfide per mol enzyme. Either ferricyanide or cytochrome c could be used as electron acceptors; the pH optimum was 7.7 with ferricyanide and 8.8 with cytochrome c. K m values for AMP and sulfite were 90 μM and 1.3 μM with ferricyanide and 91 μM and 71 μM with cytochrome c as electron acceptor. K m values for ferricyanide and cytochrome c were 89 μM and 21 μM, respectively. The properties of the enzyme were compared with those of purified adenylylsulfate reductases from other microorganisms.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-072X
    Keywords: Rhodospirillaceae ; Rhodopseudomonas adriatica ; Taxonomic characterization ; Sulfur metabolism ; Rhodobacter adriaticus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new purple nonsulfur bacterium was isolated from enrichment cultures of a sulfide-containing marine lagoon. The bacterium is similar to Rhodopseudomonas capsulata and is described as a new species of the genus Rhodopseudomonas: Rhodopseudomonas adriatica. Cells are non-motile, 0.5–0.8 μm by 1.3–1.8 μm, and multiply by binary fission. Intracytoplasmic membranes are of the vesicular type. The photosynthetic pigments are bacteriochlorophyll a and carotenoids of the spheroidene group. Growth is possible anaerobically in the light and at low pO2 in the dark. Biotin and thiamine are required as growth factors. A wide variety of organic compounds, as well as sulfide and thiosulfate, are used as photosynthetic electron donors. Sulfide is oxidized to elemental sulfur, which is subsequently converted to sulfate, whereas thiosulfate oxidation occurs without measurable intermediate. Rhodopseudomonas adriatica is unable to assimilate sulfate, growth is only possible in the presence of a reduced sulfur compound.
    Type of Medium: Electronic Resource
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