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  • 1
    Electronic Resource
    Electronic Resource
    Sporogenic Substances Isolated from Fungi (1966)
    [s.l.] : Nature Publishing Group
    Nature 212 (1966), S. 163-164 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] LIGHT is an absolute requirement for the formation of reproductive organs in many species of fungi. All the major groups of true fungi, as well as some myxomycetes, include light sensitive species1-3. Although there have been many references to the effect of light on particular species, few ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/212163a0
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  • 2
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies against the plant cytokinin isopentenyl adenosine (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 70 (1987), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Sixteen monoclonal antibodies against isopentenyl adenosine (iPA) were developed and characterized for reactivity towards this cytokinin and structurally related molecules by use of a competition fluorescence enzyme immunoassay. Antibodies with suitable affinity and specificity were used in an immunoassay to detect and quantify isopentenyl adenosine. Logit/log plots of fluorescence vs pmol of the cytokinin indicated that the assay had a measurement range of 0.03–256 pmol (10–8500 pg) with high linearity (r =–0.98). This competition fluorescence enzyme immunoassay showed that three antibodies cross-reacted with N-6-benzyladenine and its riboside: cross-reactivity with dihydrozeatin and its riboside, cis-zeatin, trans-zeatin riboside, adenine and adenosine was minor. When an immunoaffinity-HPLC technique was used to measure the cross-reactivity of two of the antibodies in the presence of known quantities of multiple cytokinins, iPA was bound in preference to the other cytokinins. One of the antibodies was used to quantify this cytokinin in developing wheat (Triticum aestivum L. cv. Stephens) seeds and in wheat seeds spiked with known amounts of certain other cytokinins. The use of these antibodies in immunoassay in combination with HPCL for quantification of iPA in plant extracts is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1987.tb02844.x
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  • 3
    Electronic Resource
    Electronic Resource
    Sulfhydryl and Disulfide Patterns Associated with Vernalization of Wheat (1967)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 20 (1967), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Analyses of plumules from seedlings of a winter wheat variety (Elgin) and a spring wheat variety (Red Bobs) indicated that SH and SS contents based on protein levels were similar in the whiter and spring wheat tissues grown at 20 C. Boll) varieties, when grown nt 3 C, contained greater concentrations of SH and SS groups, based on protein levels, than those present in the tissues grown at 20 C. The spring wheat tissues at 3 C maintained relatively constant levels of SH and SS during the growth period.Sulfhydryl (SH) and SS groups based on fresh weights were present in greater quantities in the tissues of the spring wheat grown at 20 C than in the winter wheat tissues grown at 20 C. The high SH and SS concentrations present in the warm-grown spring wheat tissues were due to a high concentration of protein extracted from that tissue. The SH and SS contents per unit fresh weight were quite similar in the cold-grown tissues of both varieties during the growth period. Both varieties at both temperatures showed a similar decrease in SH and SS contents, based on fresh weights, as maturation progressed.The spring wheats grown at 3 C and 20 C and the winter wheat grown at 3 C all showed similar characteristics and differed considerably from the winter wheat grown at 20 C. These data were positively correlated with the flowering potential of the wheat varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1967.tb07147.x
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of cytokinins in young wheat spikes (Triticum aestivum cv. Chinese spring) (1988)
    Springer
    Journal of plant growth regulation 7 (1988), S. 169-178 
    Details
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cytokinins in young wheat (Triticum aestivum cv. Chinese spring) spikes (2–15 mm) were isolated by immunoaffinity chromatography. High-performance liquid chromatography-radioimmunoassay and gas chromatography-mass spectrometry indicated that major cytokinins present weretrans-zeatin, ribosyl-trans-zeatin, ribosyldihydrozeatintrans-zeatin-9-glucoside, and the glucosides oftrans-zeatin, ribosyl-trans-zeatin, andtrans-zeatin-9-glucoside. Dihydrozeatin,iso-pentenyladenosine, andiso-pentenyladenine were also present but at lower concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02024680
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  • 5
    Electronic Resource
    Electronic Resource
    The development of monoclonal antibodies against the cytokinin zeatin riboside (1991)
    Springer
    Journal of plant growth regulation 10 (1991), S. 101-109 
    Details
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven monoclonal anti-zeatin riboside antibodies were characterized by radioimmunoassay (RIA) and found to measure femtomole (10−15 M) quantities (∼20 pg) of this cytokinin. The antibodies had different measuring ranges defined by the linear portion of the logit/log plots; slopes and intercepts of the line varied considerably between the antibodies. Competitive binding trials againstcis-zeatin riboside (cZR), dihydrozeatin riboside (diHZR), zeatin (Z), and isopentenyl adenosine (iPA) showed differences among the seven antibodies in their cross-reactivities towards these structurally related cytokinins. It was possible to combine selected antibodies to provide a mixture with a predictable measuring range and cross-reactivity; the ability to prepare a highly specific reagent in this manner with well-defined reactivity was noted and differences between monoclonal antibody and polyclonal antiserum probes for measurement of cytokinins were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02266948
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructure of primary sporidia of a wheat-bunt fungus,Tilletia caries, during ontogeny and mating (1979)
    Springer
    Protoplasma 99 (1979), S. 189-202 
    Details
    ISSN: 1615-6102
    Keywords: Mating ; Sporidia ; Tilletia ; Ultrastructure ; Wheat bunt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Primary sporidia ofTilletia caries (DC.) Tul. are borne on denticles at the tips of promycelia. The promycelia contain many small vacuoles and mitochondria and numerous lipid bodies. As the primary sporidia develop, the promycelial cytoplasm passes into the nascent cells. Septa develop between the bases of mature sporidia and the tips of the denticles. Sporidia that abscise from the denticles commonly have prominent birth scars at their bases. The sporidia have very thin walls, few vacuoles, attenuated mitochondria, and numerous lipid bodies. Conjugation pegs are generally produced by both members of a conjugating pair of sporidia and there are bud scars where they emerge from the sporidia. The sporidial walls are apparently hydrolyzed during emergence of the pegs. Vesicles are sometimes present at the tips of the conjugation pegs and, before fusion, electron-dense accumulations are sometimes observed between the tips of adjacent pegs. The approaching conjugation pegs are precisely aligned prior to fusion, suggesting polar communication. The walls of the conjugation pegs fuse and then are hydrolyzed. Fused sporidia are relatively homogeneous in content. The nucleus in a sporidum is often close to the conjugation tube and occasionally is partly within the fusion tube.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275734
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