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  • 1
    Electronic Resource
    Electronic Resource
    Genetic and biochemical characterization of a Cat2 catalase null mutant of zea mays (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 158-163 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In all maize inbred lines examined to date, the Cat2 gene which codes for the CAT-2 catalase is expressed primarily in the scutellum upon seed imbibition. The activity of CAT-2 increases dramatically during the initial four days after germination and subsequently declines. In contrast, we have recently identified and inbred strain (A16) of maize which does not express the Cat2 gene (i.e., the CAT-2 catalase is undetectable). Electrophoretic and immunological analyses indicate that the CAT-2 protein is not present in either an active or inactive form in line A16. Genetic analysis suggests that the absence of CAT-2 expression in line A16 is due to a null allele at the Cat2 gene locus although the possibility of a mutation at a regulatory locus, closely linked to the structural gene has not been excluded. Two other enzymes involved in H2O2 metabolism (superoxide dismutase and peroxidase) were also compared in W64A and A16 with no significant differences being observed. Aminotriazole (AT), a known inhibitor of catalase, has been used to simulate the A16 phenomenon by inhibiting catalase activity in line W64A (which has normal expression of CAT-2). AT, in very low concentrations, effectively inhibits the expression of CAT-2 in the scutellum. This inhibition of catalase by AT does not result in changes of the developmental time-course of superoxide dismutase and peroxidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268421
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of a catalase inhibitor in the developmental regulation of maize catalase (1979)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 1 (1979), S. 257-269 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A protein which has been shown to inhibit catalase in vitro appears to vary inversely with catalase activity in the maize scutellum during early sporophytic development when assayed using a catalase inhibition assay. This result suggested that the inhibitor protein may play a direct role in regulating catalase activity during this time period.Four experimental approaches were used to evaluate this putative regulatory role, including immunological quantitation of individual catalase isozymes during germination using rocket immunoelectrophoresis, perturbation of normal catalase expression with hydrogen peroxide or allylisopropylacetamide (AIA), examination of a mutant line with an altered catalase developmental program, and direct radioimmunoassay of the inhibitor protein during germination. The results of these experiments indicate that the quantitative changes in catalase activity during development are not mainly due to changes in the expression of the catalase inhibitor. Other possible roles of this protein in catalase regulation are discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020010308
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    Paper (German National Licenses)
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