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  • 1
    Electronic Resource
    Electronic Resource
    Evaluation of polygalacturonase activity in Saccharomyces cerevisiae wine strains (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 237 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A total of 61 S. cerevisiae strains, 60 of them isolated from wine ecosystems, were evaluated for the presence of the gene encoding endopolygalacturonase (PGU1) and for polygalacturonase (PG) activity. Nine strains lack the gene PGU1 and did not exhibit PG activity on plate assays. Of the 52 strains showing an amplified band corresponding to the size of PGU1 gene, only 36 degraded polygalacturonic acid (PGA) and 17 did not degrade it at any of the pH values used. The coding region of the PGU1 gene (ORF YJR153w) was not present in some PG activity negative strains. The S. cerevisiae UCLMS-39 strain was selected for its specific activity at different pHs, temperatures and oenological parameters. The temperature and pH optima were 50 °C and 3.5–5.5 respectively and it was only affected by ethanol. The PGU1 gene was cloned and sequenced. The production of a biologically functional endoPG in S. cerevisiae UCLMS-39 brings us a step closer to improving the qualities of outstanding enological yeasts naturally lacking PG activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09705.x
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative study of non-Saccharomyces microflora of musts in fermentation, by physiological and molecular methods (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 173 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Non-Saccharomyces yeast isolates from musts of AC (Appellation Controlee) La Mancha in spontaneous fermentation were comparatively identified by physiological and molecular tests. The criterion of Barnett for the phenotypic identification was followed. Genetic characterization by polymerase chain reaction (PCR)/restriction fragment length polymorphism (RFLP) was made. The region between 18S and 28S rRNA genes was amplified using specific internal transcribed spacers ITS1 and ITS4 primers. The 47 non-Saccharomyces isolates produced nine different phenotypic profiles and 13 different genetic profiles, showing that PCR/RFLP can be more discriminating. The information supplied by the two methodologies was very similar. PCR/RFLP can be used to correct erroneous identifications by phenotype, and in some cases to achieve intra-species differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb13506.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of wine yeasts by temperature gradient gel electrophoresis (TGGE) (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 193 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtained. Heteroduplex analysis was capable of differentiating between similar bands produced by two different species, thereby enhancing the resolution of the TGGE, yielding valuable information in a short time without the need of sequencing or complicated equipment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09400.x
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    Paper (German National Licenses)
    Fulltext
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