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  • 1
    ISSN: 1432-0789
    Keywords: Acacia mangium ; P fertilization ; Root nodulation ; N2 fixation ; Acetylene reduction assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant growth performance, the P content in root and nodule tissues, and nodulation and N2-fixing ability were studied in four provenances of Acacia mangium from Papua New Guinea following different levels of P fertilizer application. A. mangium did not seem to need high levels of P for growth and N2 fixation. The response by this leguminous tree to the P supply varied significantly according to provenance and to P concentrations in the culture solution. The provenances of A. mangium were classified into three types according to their P response: (1) Growth performance, nodulation, and N2 fixation of plants were stimulated as concentrations of P increased (provenance PH 482); (2) the maximal effect of P on plant growth was found only at P concentrations higher than 500 μM (provenance PH 484); and (3) the plant response to P fertilization was low, even with nutrient solutions containing P concentratins higher than 500 μM (provenances PH 483 and PH 485). Provenance PH 483 was distinguished by its low nodulating ability. However, this provenance grew well, probably because of its high N2 fixation efficiency as expressed by specific acetylene reduction activity and its high P content in nodule tissues. Therefore, in certain cases, these two parameters may be useful criteria in selecting leguminous plants for field use. Statistical analyses of the study results showed that the effect of the factor P supply on N2 fixation efficiency and nodule development was only significant at P concentrations lower than 250 μM whereas the effect of the factor plant provenance was significant regardless of the P concentration used. This observation emphasizes the value of provenance screening in the identification of plants for use in a wide range of soil types.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 23 (2000), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Water deficit is a very serious constraint on N2 fixation rates and grain yield of soybean (Glycine max Merr.). Ureides are transported from the nodules and they accumulate in the leaves during soil drying. This accumulation appears responsible for a feedback mechanism on nitrogen fixation, and it is hypothesized to result from a decreased ureide degradation in the leaf. One enzyme involved in the ureide degradation, allantoate amidohydrolase, is manganese (Mn) dependent. As Mn deficiency can occur in soils where soybean is grown, this deficiency may aggravate soybean sensitivity to water deficit. In situ ureide breakdown was measured by incubating soybean leaves in a 5 mol m−3 allantoic acid solution for 9 h before sampling leaf discs in which remnant ureide was measured over time. In situ ureide breakdown was dramatically decreased in leaves from plants grown without Mn. At the plant level, allantoic acid application in the nutrient solution of hydroponically grown soybean resulted in a higher accumulation of ureide in leaves and lower acetylene reduction activity (ARA) by plants grown with 0 mol m−3 Mn than those grown with 6·6 mol m−3 Mn. Those plants grown with 6·6 mol m−3 Mn in comparison with those grown with 52·8 mol m−3 Mn had, in turn, higher accumulated ureide and lower ARA. To determine if Mn level also influenced N2 fixation sensitivity to water deficit, a dry-down experiment was carried out by slowly dehydrating plants that were grown in soil under four different Mn nutritions. Plants receiving no Mn had the lowest leaf Mn concentration, 11·9 mg kg−1, and had N2 fixation more sensitive to water deficit than plants treated with Mn in which leaf Mn concentration was in the range of 21–33 mg kg−1. The highest Mn treatments increased leaf Mn concentration to 37·5 mg kg−1 and above but did not delay the decline of ARA with soil drying, although these plants showed a significant increase in ARA under well-watered conditions.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 110 (2000), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Inhibition of N2 fixation under water deficits has been hypothesized to result from N feedback within the plant involving ureides and/or asparagine (Asn). This study was undertaken to investigate ureide and Asn accumulation in shoots and nodules in response to treatments inhibiting nodule activity (acetylene reduction assay, ARA) such as Asn, ureide, or polyethylene glycol application to the nutrient solution of plants, boric acid on leaves, and imposition of a water deficit. ARA inhibition and nodule concentration of Asn and ureide were correlated to the ureide treatment applied (3–15 mM applied in the nutrient solution). Supplying Asn (3–9 mM applied in the nutrient solution) also induced an increase in nodule Asn and ureide concentration, which was associated with ARA inhibition. Spraying boric acid on leaves also inhibited ARA in parallel to an increase in shoot ureide and nodule Asn concentration while nodule ureide remained unchanged. By contrast, polyethylene glycol (PEG) inhibited ARA in parallel to an increase in nodule Asn and ureide concentration while shoot ureide was unchanged. The decline in ARA in response to water deficit was associated with an increase in nodule ureide, Asn and aspartate (Asp), although the increases in Asn and Asp were less than for ureides. Altogether, the results of these experiments indicated that Asn cannot be the only compound involved in the feedback inhibition of ARA. Instead ureide and Asn are probably both involved, either directly by accumulation of products that fail to be exported, or by feedback from the shoot due to an N-compound supply that exceeds shoot requirements.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; Phosphorus deficiency ; Phosphorus use efficiency ; it Rhizobium ; Symbiotic nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Common bean often faces P deficiency in soils where it is generally grown. Such a deficiency is a major limitation to grain yield improvement, especially when common bean relies on N2 fixation. Screening for symbiotic N2 fixation (SNF) under P deficiency (72 μmol P plant-1 week-1) was performed in a glasshouse with 220 lines originating from the Andean and Meso-American gene pools. Large variability in shoot dry weight and SNF under P deficiency was found. SNF tolerance to P deficiency was mostly found among late flowering type IV lines, with the exception of three early flowering type III lines. The SNF tolerance to P deficiency was correlated with: (i) low nodule P concentration in all the growth types, and (ii) intense and early nodulation for type IV lines, or (iii) large root dry weight and high nodule nitrogenase for type III lines. Large genotypic variability for nitrogen use efficiency, i.e. biomass per N concentration was also found. However, the ratio plant N per plant P concentration was the most discriminating. We concluded that this later ratio, which is almost similar to plant N-fixed per plant P concentration, in glasshouse hydroponics culture might be: (i) an estimate of the P use efficiency for SNF, (ii) a consequence of low P concentration in plant organs, especially nodules, and (iii) a determinant of genotypic variability of SNF tolerance to P deficiency.
    Type of Medium: Electronic Resource
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