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  • 1
    Electronic Resource
    Electronic Resource
    Enzyme-linked immunosorbent assay (ELISA) detection of infectious pancreatic necrosis virus (IPNV) in culture fluids and tissue homogenates of the rainbow trout, Salmo gairdneri Richardson (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 11 (1988), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. A double antibody enzyme-linked immunosorbent assay (ELISA) for the detection of infectious pancreatic necrosis virus (IPNV) is described. The sensitivity of the assay reached 102 TCID50 per 0·1 ml of culture fluid. The specificity of anti-IPNV sera and of the assay was confirmed by agar-gel immunodiffusion, by the direct immunoperoxidase technique for the deletion of IPNV in tissue cultures and by the ELISA inhibition test. High values of specific inhibition (over 90% at serum dilutions 1:40–1:2560) and low values of non-specific inhibition (8·4% at serum dilution 1:160) demonstrated the quality of the rabbit anti-IPNV serum. The results of ELISA agreed well with those of virological examinations. The potential of ELISA for investigations of a large series of field samples is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1988.tb00543.x
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  • 2
    Electronic Resource
    Electronic Resource
    Enzyme-linked immunosorbent assay (ELISA) for the detection of spring viraemia of carp virus (SVCV) in tissue homogenates of the carp, Cyprinus carpio L. (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 16 (1993), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. An enzyme-linked immunosorbent assay (ELISA) for the demonstration of the virus of spring viraemia of carp (SVCV) in liver, kidney and spleen homogenates, and in infected cell cultures is described. The sensitivity of the method is 102·8–103·5 TCID50 0·lml−1 of the examined fluid. The specificity has been confirmed by the ELISA inhibition test and by results of virological examinations. Contamination with bacteria or fungi of samples taken from dead fish had no effect on the results of ELISA. Specific anti-SVCV sera were used successfully for the production of conjugates for the direct immunoperoxidase and immunofluorescence detection of SVCV in infected cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1993.tb00853.x
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  • 3
    Electronic Resource
    Electronic Resource
    Freeze-etching observations on infectious bovine rhinotracheitis virus in the nucleus of bovine kidney cells (1977)
    Springer
    Archives of virology 53 (1977), S. 167-170 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study of infectious bovine rhinotracheitis (IBR) virus in the nucleus of infected bovine kidney cells using the freeze-etching technique revealed filaments and granular viral matrix, core-like particles and various developmental forms of the virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314858
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  • 4
    Electronic Resource
    Electronic Resource
    Electron and immunoelectron microscopy of rabbit haemorrhagic disease virus (RHDV) (1990)
    Springer
    Archives of virology 112 (1990), S. 271-275 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rabbit haemorrhagic disease virus (RHDV) had a calicivirus-like structure and a diameter of 31.5–33.0 nm. Antigenic relationship between the investigated RHDV strain and the causal agent of RHD in China was demonstrated by immunoelectron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01323171
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  • 5
    Electronic Resource
    Electronic Resource
    Envelopment and the envelopes of infectious bovine rhinotracheitis virus in ultrathin sections (1976)
    Springer
    Archives of virology 51 (1976), S. 131-140 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An electron microscopic study of cell cultures and bovine foetal tracheal organ cultures infected with infectious bovine rhinotracheitis (IBR) virus showed the following. a) The difference in the site at which the outer envelope is acquired in the infected cell is responsible for some morphological differences between the virions. Where envelopment of the capsids occurs by budding into cytoplasmic tubules, dense material adjacent to the tubules is often incorporated between the capsid and the outer envelope, giving a pleomorphic appearance to the latter. No dense material is seen in the same position if envelopment occurs in the nucleus. b) Dense material adjacent to the cytoplasmic tubules is not only incorporated in the virions, but may also bud into the cytoplasmic tubules without nucleocapsids, thus giving rise to dense bodies. c) Dense material in the virions is closely adjacent to the inner side of the outer envelope, but is separated from the capsid by a lucent zone. A similar zone can be seen around some nucleocapsids in the nucleus. d) The outer envelope of numerous extracellular virions and of those located in the cytoplasmic tubules shows the unit membrane structure with projections. The outer envelope of intranuclear particles and of those located in the perinuclear cisterna had the appearance of a dense membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317842
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  • 6
    Electronic Resource
    Electronic Resource
    Porcine cytomegalic inclusion disease virus (1970)
    Springer
    Archives of virology 32 (1970), S. 19-30 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An electron microscopic study was made on porcine cytomegalic inclusion disease virus in the nasal mucosa of three piglets from a herd with a history of generalized cytomegalic inclusion disease. In addition to previously described viral developmental forms within the nucleus and enveloped virus particles between the nuclear membranes, the study revealed enveloped virus particles within membrane-enclosed clusters in the nucleus, and reduplication of nuclear membranes. In the cytoplasm, enveloped virus particles, 120–150 mΜ in diameter, were found within vacuoles. Unenveloped virus particles, 90–110 mΜ in diameter, were located free in the cytoplasm and often showed a crystal pattern. In the nucleus, the crystal pattern of virus particles could not be detected. In the cytoplasm, various viral forms were also found within a dense osmiophilic matrix which is regarded as the site of virus destruction. The mode of acquiring viral envelopes within the nucleus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241515
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Electron microscopy of porcine cytomegalovirus (1973)
    Springer
    Archives of virology 41 (1973), S. 344-353 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Porcine cytomegalovirus was examined by electron microscopy in the nasal mucosa of piglets from a natural epizootic of the disease with the object to determine the localization of nucleocapsids arranged in crystalline arrays in the cell and to investigate the structure of most common viral forms. The study revealed the following. 1. The morphogenesis of porcine cytomegalovirus is marked by intranuclear formation of nucleocapsids arranged in crystalline arrays. Large quantities of crystals found in the cytoplasm were apparently extruded from the nucleus at late stages of infection; 2. nucleocapsids in the cytoplasm were coated with an electron dense amorphous material. This coat was also seen at the surface of capsids in cytoplasmic and extracellular enveloped particles, but was absent from the surface of capsids located in the nucleus; 3. the majority of enveloped virus particles showed an electron translucent zone separating the capsid from the envelope, and 4. the outer envelope of cytoplasmic and extracellular particles had projections and showed a clear unit membrane structure, whereas the outer envelope of intranuclear particles and those located in the perinuclear cisterna had the appearance of an electron dense single membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01250206
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