ISSN:
0886-1544
Keywords:
α-spectrin
;
coelomocytes
;
filopodia
;
actin/membrane interactions
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
We have investigated the presence and localization of an α-spectrinlike protein and its potential role in the morphological transformation of sea urchin coelomocytes. In immunofluorescence images there is a diffuse fluorescence throughout the petaloid cytoplasm, indicating a random distribution of the spectrinlike protein prior to the transformation. As these cells form filopodia, there is a coincident appearance of a spectrinlike protein, as seen in fluorescent images, at the site of filopodial initiation. As the filopodia continue to form and lengthen, the spectrin localization parallels their development. There is a single polypeptide observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels of whole coelomocyte lysates that cross-reacts with the anti-α-spectrin immunogen and comigrates with it at 240 kilodaltons.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970030532
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