ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Nine horse and five calf normal sera were studied for their antiviral activity against ECHO 1, 2, 4, 7, 9 and polio type 1 viruses, both by the plaque reduction test and by the kinetics of neutralization. These two activities appear to be due to different substances, because there was no correlation between the inhibiting and the neutralizing properties. Moreover experiments performed with two sera demonstrated that the inhibiting activity was unaffected by 2-ME treatment, whereas the neutralizing one was almost abolished. The plaque inhibitors found in the tested sera against polio type 1 virus were not destroyed by heating at 56°C for 30 minutes and completely eliminated by heating at 80°C for 15 minutes. Periodate treatment did not have any effect on the inhibiting property. The inhibitor could be demonstrated in the gamma globulin fraction, obtained from the sera by ammonium sulfate precipitation, but from our results it is not possible to make inferences on the chemical nature of the inhibitor or inhibitors. The neutralizing effect was clearly associated with an antibody-like substance. This conclusion was based upon: 1. the type of the kinetics of neutralization 2. the specificity of the neutralizing activity 3. the sensitivity to 2-ME treatment, and 4. the fact that resistant mutants could not be obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01258163
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