ISSN:
1573-8264
Keywords:
alginate
;
carrot
;
cell permeabilization
;
glutaraldehyde
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Carrot cell suspension culture prior to immobilization by glutaraldehyde was permeabilised by Tween 80. The values of pH optimum for L-alanine aminopeptidase, L-proline iminopeptidase, and L-arginine aminopeptidase were 8.2, 7.4, and 7.9, respectively. The immobilized cells showed significantly lower aminopeptidase activity when compared to untreated cells. Alginate hydrogel was successfully used for immobilization of carrot cells retaining the activity of some aminopeptidases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026756124363
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