ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The reproducibility of ion abundances in the fast atom bombardment experiment was evaluated by comparing mass spectral data obtained with the same sample analysed by eight different laboratories. Instrumentation included magnetic sector and quadrupole analysers and several different types of atom guns for the primary particle beam. A special fast atom bombardment gun was constructed which, by design, did not include ions or electrons in the particle beam. In addition this atom gun had a 0.1 eV beam energy spread and could be focused. The molecules in the study included dihexadecanoyl phosphatidyl choline, bradykinin, oxidized glutathione, sulfotaurolithocholic acid, ATP, NAD+ and stachyose. Major differences in the FAB data between laboratories were in the absolute signal-to-noise and abundance of glycerol-cluster ions. The relative abundances for analyte-related ions were very reproducible in spite of major instrumental differences. The coefficients of variation for analyte-ions included in the study typically ranged from 20-80% which was similar to those found for the electron impact mass spectrum of methyl stearate obtained by these laboratories. These data suggest that the relative intensities of analyte-related ions obtained fast atom bombardment mass spectrometry are as reproducible as electron impact mass spectrometry.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200100902
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