ISSN:
1573-2576
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Extracellular adenosine has pharmacological activity on a wide variety of cell types and may play an important role as an inflammatory modulator with both pro- and anti-inflammatory activities. These studies examine the effects of adenosine on guinea pig pulmonary eosinophils. Adenosine alone did not directly induce superoxide (O 2 − ) production. Pretreatment with adenosine primed the O 2 − response of guinea pig pulmonary eosinophils following the addition of 1 or 10μM plateletactivating factor (PAF). Priming was seen at adenosine concentrations greater than 1 μM and was maximal at 100μM. At this maximal dose, adenosine priming increased the O 2 − response to 1μM and 10μM PAF by 86% and 51%, respectively. Priming by adenosine was not seen when ionomycin or phorbol myristate acid (PMA) were used as agonists. In fura-2 loaded eosinophils, the addition of 100 μM adenosine resulted in a small but significant rise in intracellular calcium of 54.4 ±9.2 nM above baseline. In contrast, similar adenosine concentrations had no effect on cytosolic calcium levels in guinea pig neutrophils. These data demonstrate a pro-inflammatory role for adenosine in elicited guinea pig pulmonary eosinophils.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01487741
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