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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 36 (1971), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY –EDTA, in concentrations above 2.5 mM, was found inhibitory to germination of and outgrowth from spores of C. botulinum Type A and to toxin production in a fish homogenate. Inhibitory action was influenced by pH of the medium in the range pH 6.5–8.1, the action increasing with pH. It was influenced by Mg and Ca concentrations in the medium, equimolar concentrations of added CaCl2 or MgCl2, completely erasing the growth inhibitory action. Initial spore concentration also influenced inhibitory efficacy–the higher the spore concentration, the higher the EDTA concentration required for inhibition. There was no evidence that EDTA, in any concentration used, promoted spore germination. Release of Ca, Mg and DPA from incubating spores was suppressed to varying extents by 5.0 and 10 mM EDTA.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 36 (1971), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Death of spores of Clostridium botulinum when exposed to gaseous ethylene oxide followed first order kinetics. Supplementation of a synthetic medium with the purine and pyrimidine bases of DNA and RNA indicated, as judged by outgrowth from spores which had received sublethal ETO treatments, that the lethal action of ETO on the spores was through alkylation of the guanine and adenine components of DNA. Observed impairment of RNA and protein synthesis was considered an indirect effect resulting from alkylation of DNA components; however, additional evidence bearing on this point is needed to support a more definite conclusion.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 101 (1988), S. 141-155 
    ISSN: 1573-0832
    Keywords: ragi ; murcha ; bubod ; Chinese yeast ; chlamydospores ; microbial counts of amylase starters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ragi and ragi-like starters were obtained from China, India, Indonesia (Java and Bali), Malaysia, Nepal, Philippines, and Taiwan. These starters have a number of names in each country (murcha, bubod, Chinese yeast). The microorganisms found in 41 samples examined were 3 genera of the Mucorales (Rhizopus, Mucor, Amylomyces), yeasts and bacteria. Except for a few chance contaminants only Mucoraceous fungi were found, and the appearance and growth of colonies of yeasts and bacteria indicate that only 2 or 3 species of each were present. The starters from different countries sold under different names are identical in their flora except that Amylomyces was rarely, if ever, a part of the murcha flora in samples from Nepal. The range in counts were 4x103 to 2.1×108 bacteria, 4×103 to 6.1×108 for molds and yeasts, and 3×103 to 6.1×108 for yeasts alone. The anaerobic count ranged from 3×102 to 1.5×108 and was made up of both yeasts and bacteria. Every sample contained yeast and at least one Mucoraceous mold. Amylomyces was shown to survive long periods of time — as many as 5 years at room temperature resulted in retained amylolytic activity. There was a considerable reduction in isolates of Mucor and Rhizopus with long periods of storage. Chlamydospore germination was seen for the first time in Amylomyces.
    Type of Medium: Electronic Resource
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