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  • 1
    Electronic Resource
    Electronic Resource
    Evidence for the Transfer of Rhizobial Metabolites to the Polyploid Nuclei of Young Clover Nodules (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 27 (1972), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Autoradiography was used to provide evidence for the transfer of Rhizobium produced moieties to the host nuclei of young clover nodule cells. Cells of Rhizobium trifolii Dangeard were labeled with 3H-adenine or 4,5-3H-l-leucine and 3,4-3H-l.-proline, washed free of external label, and allowed to nodulate young seedlings of white clover (Trifolium repens L. cv. White Dutch). Sections (0.5–1.0 μm) of young nodules up to four days old were autoradiographed using the dipping technique. Grain counts indicated movement of tritium from the leucine-proline labeled rhizobia to the polyploid nuclei of two day old clover nodule cells. Acetylene reduction was not detected until approximately 24 hours after the transfer of tritium was observed. No transfer of tritium was observed with 3H-adenine labeled rhizobia. It is hypothesized that nodulating rhizobia may induce clover nodule cells to initiate leghemoglobin synthesis by the transfer of a bacterially produced inducer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1972.tb03609.x
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  • 2
    Electronic Resource
    Electronic Resource
    Water uptake and storage by rhizosheaths of Oryzopsis hymenoides: a numerical simulation (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 65 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Rhizosheaths (sheaths of sand grains that form around the roots of some grasses) are common in perennial grasses that colonise sandy substrates. It has been hypothesised that rhizosheaths increase water availability by increasing the efficiency of water absorption. Others have suggested that rhizosheaths act as storage reservoirs for water. In either case rhizosheaths undoubtedly play an important role in the water relations of these grasses.In an attempt to evaluate the main function of rhizosheaths, we developed a finite element cylindrical water flow model which enabled us to simulate water uptake by Oryzopsis hymenoides (Roem, and Shult.) Ricker. This model allowed us to estimate total water uptake by root systems with and without rhizosheaths and to compare these values to the extra water stored within the rhizosheath. The results of this study suggest that the presence of rhizosheaths is more important in reducing the total resistance to water flow within the rhizosphere than in enhancing water storage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb02387.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and Partial Characterization of DNA in Capsular Preparations of Rhizobium trifolii (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 35 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The capsular polysaccharides from thymidine-(methyl-3H) labeled cultures of Rhizobium trifolii; strain 162S7 (Nitragin Co.) were centrifuged from bacterial cells and collected by ethanol precipitation. Following the addition of unlabeled carrier nucleic acids, labeled DNA, termed cap-DNA, was isolated from the capsular polysaccharides. Cap-DNA absorbed maximally at H-260 nm and was DNase sensitive. Approximately 11 μg of 3H-cap-DNA were consistently isolated per liter of 48 h cultures. Cap-DNA production was generally synchronized with the synthesis of the capsular polysaccharide and bacterial growth, attaining maximum recoverable amounts in 48 h cultures. By five days of culture growth, significant decreases in the amount of recoverable cap-DNA were noted. The presence of label in the cap-DNA demonstrated that the cap-DNA originated via de novo synthesis by the Rhizobium cells rather than from an anomalous source. The cap-DNA and intracellular Rhizobium DNA had similar buoyant densities of p= 1.719, indicating that cap-DNA arose specifically from the intracellular DNA. In 48-h cultures the specific activity of the cap-DNA was about one-third that of the intracellular DNA. This implies intracellular DNA was released during early growth with a relatively low specific activity which diluted the isotopic label of DNA released later. The evidence suggests lysogeny was the principal release mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03881.x
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  • 4
    Electronic Resource
    Electronic Resource
    Nitrogen Fixation Associated with Sand Grain Root Sheaths (Rhizosheaths) of Certain Xeric Grasses (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 46 (1979), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Nitrogen fixation (acetylene reduction) was found to be associated with sand grain root sheaths (rhizoseaths) occurring on the following xeric grasses: Oryzopsis hymenoides (Roem. and Shult.) Ricker, Agropyron dasystachyum (Hook.) Scrib., Stipa comata Trin. and Rupr., and Aristida purpurea Nutt. Acetylene reduction rates associated with whole plant specimens of these species varied from 515 to 920 nmol C2H4/(g dry wt.) × (6 days). Nitrogenase activity was shown to be associated with the rhizosheaths. Bacillus polymyxa-like nitrogen fixers were isolated from the rhizosheaths of each grass. The isolates reduced acetylene and assimilated 15N2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1979.tb03176.x
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  • 5
    Electronic Resource
    Electronic Resource
    Non-enzymatic Formation of Nitrogen Gas (1966)
    [s.l.] : Nature Publishing Group
    Nature 210 (1966), S. 1150-1151 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] All experiments were carried out in electrolytic respiro-meter flasks with gas analyses being made with a Beekman '0(7-2' gas chromatograph6. pH determinations were made using a Beekman 'model G' p~H. meter. The following reagents were used: aqueous potassium nitrite equivalent to 10,000 p.p.m. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2101150a0
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    Paper (German National Licenses)
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