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  • 1
    Electronic Resource
    Electronic Resource
    Gas chromatographic and mass spectrometric analysis of molecular species of bacterionemamycolic acids from Bacterionema matruchotii (1981)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 11 (1981), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1981.tb06960.x
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  • 2
    Electronic Resource
    Electronic Resource
    Low-temperature-induced desaturation of fatty acids and expression of desaturase genes in the cyanobacterium Synechococcus sp. PCC 7002 (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 152 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Changes in response to temperature of lipid classes, fatty acid composition and mRNA levels for acyl-lipid desaturase genes were studied in the marine unicellular cyanobacterium, Synechococcus sp. PCC 7002. The degree of unsaturation of C18 fatty acids increased in cells grown at lower temperature for all lipid classes, and ω3 desaturation occurred specifically in cells grown at low temperature. While the level of 18:1(9) fatty acids declined, desaturation at the ω3 position of C18 fatty acids increased gradually during a 12-h period after a temperature shift-down to 22°C. However, the mRNA levels of the desA (Δ12 desaturase), desB (ω3 desaturase) and desC (Δ9 desaturase) genes increased within 15 min after a temperature shift-down to 22°C; the desaturase gene mRNA levels also rapidly declined within 15 min after a temperature shift-up to 38°C. Therefore, the elevation of mRNA levels for the desaturase genes is not the rate-limiting event for the increased desaturation of membrane lipids after a temperature shift-down. The rapid, low-temperature-induced changes in mRNA levels occurred even when cells were grown under light-limiting conditions for which the growth rates at 22°C and 38°C were identical. These studies indicate that the ambient growth temperature, and not some other growth rate-related process, regulates the expression of acyl lipid desaturation in this cyanobacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb10445.x
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  • 3
    Electronic Resource
    Electronic Resource
    Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation (1990)
    [s.l.] : Nature Publishing Group
    Nature 347 (1990), S. 200-203 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A mutant in fatty-acid desaturation of membrane lipids of the transformable cyanobacterium, Synechocystis PCC6803, was isolated as described previously6. This mutant, termed Fad 12, is defective in the activity of desaturation that introduces a second double bond at the A12 position of the C18 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/347200a0
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  • 4
    Electronic Resource
    Electronic Resource
    Heat shock protein synthesis of the cyanobacterium Synechocystis PCC 6803: purification of the GroEL-related chaperonin (1992)
    Springer
    Plant molecular biology 18 (1992), S. 327-336 
    Details
    ISSN: 1573-5028
    Keywords: chaperonin ; heat shock protein (HSP) ; HSP70 ; HSP64 ; Rubisco-binding protein ; Synechocystis PCC 6803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synechocystis PCC 6803 cells could be induced to synthesize four major HSPs with apparent molecular sizes of 70, 64, 15 and 14 kDa. Heat stress at 42.5 °C appeared to be the optimum temperature for HSP formation in cells grown at 30 °C. The relative rate of synthesis of HSP70 and HSP15 reached a maximum at 30 min after the temperature shift-up whereas the capability of cells to accumulate HSP64 and HSP14 continued through 2 h. The two most abundant HSPs, HSP70 and HSP64, were recognized on western blots by antibodies raised against authentic DnaK and GroEL from Escherichia coli. To furnish sufficient evidence for the assumption that HSP64 is a GroEL-related chaperonin, this protein was purified to homogeneity. There was a 76% sequence identity between the amino acid sequence of HSP64 and the corresponding protein in Synechococcus PCC 7942. Moreover, the purified HSP64 cross-reacted to anti-E. coli GroEL antibody. To our knowledge, this is the first report about the purification and partial protein sequencing of a cyanobacterial chaperonin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034959
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning of ω3 desaturase from cyanobacteria and its use in altering the degree of membrane-lipid unsaturation (1994)
    Springer
    Plant molecular biology 26 (1994), S. 249-263 
    Details
    ISSN: 1573-5028
    Keywords: desB gene ; desaturase ; fatty acid ; Synechococcus sp. PCC 7942 ; Synechocystis sp. PCC 6803 ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanobacteria respond to a decrease in temperature by desaturating fatty acids of membrane lipids to compensate for the decrease in membrane fluidity. Among various desaturation reactions in cyanobacteria, the desaturation of the ω3 position of fatty acids is the most sensitive to the change in temperature. In the present study, we isolated a gene, designated desB, for the ω3 desaturase from the cyanobacterium, Synechocystis sp. PCC 6803. The desB gene encodes a protein a 359 amino-acid residues with molecular mass of 41.9 kDa. The desB gene is transcribed as a monocistronic operon that produced a single transcript of 1.4 kb. The level of the desB transcript in cells grown at 22°C was 10 times higher than that in cells grown at 34°C. In order to manipulate the fatty-acid unsaturation of membrane lipids, the desB gene in Synechocystis sp. PCC 6803 was mutated by insertion of a kanamycin-resistance gene cartridge. The resultant mutant was unable to desaturate fatty acids at the ω3 position. The desA gene, which encodes the Δ12 desaturase of Synechocystis sp. PCC 6803, and the desB gene were introduced into Synechococcus sp. PCC 7942. Whilst the parent cyanobacterium can only desaturate membrane lipids at the Δ9 position of fatty acids, the resultant transformant was able to desaturate fatty acids of membrane lipids at the Δ9, Δ12 and ω3 positions. These results confirm the function of the desB gene and demonstrate that it is possible to genetically manipulate the fatty-acid unsaturation of membrane lipids in cyanobacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039536
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of conserved domains in the Δ12 desaturases of cyanobacteria (1994)
    Springer
    Plant molecular biology 24 (1994), S. 643-650 
    Details
    ISSN: 1573-5028
    Keywords: Anabaena variabilis ; fatty-acid desaturation ; Synechococcus PCC7002 ; Synechococcus PCC7942 ; Synechocystis PCC6714 ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanobacterial genes for enzymes that desaturate fatty acids at the Δ12 position, designated desA, were isolated from Synechocystis PCC6714, Synechococcus PCC7002 and Anabaena variabilis by crosshybridization with a DNA probe derived from the desA gene of Synechocystis PCC6803. The genes of Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis encode proteins of 349, 347 and 350 amino acid residues, respectively. The transformation of Synechococcus PCC7942 with the desA genes from Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis was associated with the ability to introduce a second double bond at the Δ12 position of fatty acids. The amino acid sequence of the products of the desA genes revealed the presence of four conserved domains. Since one of the conserved domains was also found in the amino acid sequences of ω3 desaturases of Brassica napus and mung bean, this domain may play an essential role in the introduction of a double bond into fatty acids bound to membrane lipids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023560
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  • 7
    Electronic Resource
    Electronic Resource
    EgBridge Version 2.2 (1987)
    Springer
    Machine translation 2 (1987), S. 55-58 
    Details
    ISSN: 1573-0573
    Source: Springer Online Journal Archives 1860-2000
    Topics: Linguistics and Literary Studies , Computer Science
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01540134
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  • 8
    Electronic Resource
    Electronic Resource
    Anomalous polar substituent effect on 15N NMR chemical shifts of Aniline derivatives. Acylanilides and related compounds (1994)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 7 (1994), S. 280-286 
    Details
    ISSN: 0894-3230
    Keywords: Organic Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The 15N chemical shifts of various m- and p-substituted anilides and related derivatives, ZNHC6H4Y [Z = C6H5CO, CH3CO, CF3CO, CH3SO2, (C6H5O)2PO and H], were correlated with σ- constants. With all series of anilides investigated, the plotted points for the m- and p-substituted derivatives tend to be separated from each other and to lie on two different regression lines. Reinvestigation from this point of view revealed that similar separations occur generally with substituted aniline derivatives. The anomalous polar substituent effect was ascribed to the very large contribution of a resonance effect. In addition, the local π-polarization effect causes a negative slope for the m-substituted series in the case of carboxylic anilides.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/poc.610070603
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  • 9
    Electronic Resource
    Electronic Resource
    Multinuclear magnetic resonance and molecular mechanics studies on the conformations and the dynamic behaviors of α-alkyl-substituted o-methoxybenzyl alcohols (1997)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 10 (1997), S. 925-934 
    Details
    ISSN: 0894-3230
    Keywords: multinuclear magnetic resonance ; molecular mechanics ; MM3 ; conformation ; methoxybenzyl alcohols ; rotational barrier ; substituent effect ; steric effect ; Chemistry ; Theoretical, Physical and Computational Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: ---The conformations and the dynamic behaviors of α-monoalkyl- and α,α-dialkyl-o-methoxybenzyl alcohols are discussed on the basis of their 1H, 13C and 17O NMR spectra and MM3 calculations. The conformations of α-monoalkyl derivatives are shown to be different from those of α,α-dialkyl derivatives and unfavorable to form intramolecular OH · · · O hydrogen bonds. The trends of substituent effects on the restricted rotation about the Cipso - Cα bond could be reproduced fairly well by MM3 calculations. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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