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  • 1
    Electronic Resource
    Electronic Resource
    Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa (1980)
    Springer
    Development genes and evolution 189 (1980), S. 171-180 
    Details
    ISSN: 1432-041X
    Keywords: Amino acyl-tRNA synthetases ; Development ; Nuclear-mitochondrial interactions ; Neurospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The specific activities of the branched chain amino acyl-tRNA synthetases from the cytosolic and mitochondrial fractions ofN. crassa were low in dormant conidia and increased during germination, reaching a maximum 8 h after inoculation. This stage of development is characterised by high rates of many other cellular activities. The increases in activity of synthetases of both cytosol and mitochondria are inhibited by cycloheximide indicating that they are synthesized on cytoplasmic ribosomes. The mitochondrial synthetases show a stimulation of their specific activity when mitochondrial RNA and protein synthesis are inhibited by either ethidium bromide or chloramphenicol suggesting that a mitochondrial translation product regulates the synthesis of the mitochondrial synthetases. The activities of amino acyl-tRNA synthetases are dependent on energy production. When respiration is uncoupled from oxidative phosphorylation, synthetase specific activities decrease although the activities of other mitochondrial enzymes like NADH-dehydrogenase increase. This phenomenon suggests that more than one mechanism regulates the synthesis of mitochondrial proteins which are formed on cytoplasmic ribosomes. The synthesis of branched chain amino acyl-tRNA synthetases ofNeurospora is neither repressed by their cognate amino acids, nor is there inhibition by the precursors of these amino acids, as has been observed in other amino acyl-tRNA synthetases of various organism includingNeurospora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00868675
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  • 2
    Electronic Resource
    Electronic Resource
    Isozymes in Insects and their Significance (1974)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Entomology 19 (1974), S. 117-138 
    Details
    ISSN: 0066-4170
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.en.19.010174.001001
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mutation and Enzyme Function in Humans (1975)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 9 (1975), S. 187-212 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ge.09.120175.001155
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  • 4
    Electronic Resource
    Electronic Resource
    Growth, respiratory, and cytochrome characteristics of certain of the isoleucine-valine mutants of Neurospora crassa (1974)
    Springer
    Biochemical genetics 12 (1974), S. 39-49 
    Details
    ISSN: 1573-4927
    Keywords: Neurospora crassa ; cytochromes ; mitochondria ; respiration ; isoleucinevaline biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Several of the isoleucine-valine requiring mutants of Neurospora crassa have been shown to have differences in growth rate, oxygen uptake rate, cyanide sensitivity, and cytochrome spectral patterns as compared to wild type. This pleiotropic effect seems to be true for mutants with changes at all three known loci affecting isoleucine and valine biosynthesis, the iv-1, iv-2, and iv-3 loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00487526
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  • 5
    Electronic Resource
    Electronic Resource
    Deficiency of dihydroxy acid dehydratase in the mito-chondria of the iv-1 mutants of Neurospora crassa (1970)
    Springer
    Biochemical genetics 4 (1970), S. 243-251 
    Details
    ISSN: 1573-4927
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The isoleucine-valine requiring mutants of Neurospora crassa which map at the iv-1 locus lack, or have a very low level of activity for, the enzyme dihydroxy acid dehydratase in the mitochondrial fractions derived from them. This enzyme is, however, present in the soluble fractions of the mutant homogenates. The enzyme is present in both mitochondrial and soluble fractions from homogenates of wild-type and from homogenates of iv mutants blocked at other steps in the isoleucine-valine pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00485775
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro mitochondrial complementation in Neurospora crassa (1971)
    Springer
    Biochemical genetics 5 (1971), S. 549-561 
    Details
    ISSN: 1573-4927
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mitochondrial isoleucine-valine biosynthesis in strain 330a, an iv-1 mutant of Neurospora, is blocked at the dihydroxy acid dehydration step owing to a mutation in the nuclear structural gene for the specific enzyme dihydroxy acid dehydratase. Dehydratase purified from either the soluble or the mitochondrial fraction of wild-type Neurospora, and incubated in vitro with 330a mitochondria, restores valine synthesis from pyruvate-C 14 to wild type levels. Up to 29% of the restored synthesis could be attributed to the penetration of enzyme into the mitochondria. However, the bulk of the restored synthesis was found to be mediated via the secretion of dihydroxyvaline (DHV) by the mitochondria into the assay milieu, with subsequent enzymatic catalysis of this metabolite to ketovaline occurring outside the organelle. The ketovaline apparently diffuses back into mitochondria for final transamination to valine. This shunting of valine precursors in and out of mitochondria has been demonstrated to be the mechanism whereby two different populations of mitochondria isolated from mutants 330a and 305A (an iv-2 mutant lacking a functional reductoisomerase) can complement each other for the biosynthesis of valine, even when each population is enclosed in a separate dialysis bag. This observation provides the basis for a biochemical understanding of the growth complementation at the organismic level when these two iv-requiring mutants are cultured together in minimal medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00485673
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    Paper (German National Licenses)
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