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  • 1
    Electronic Resource
    Electronic Resource
    Association of stem cell factor expression in nasal polyp epithelial cells with aspirin sensitivity and asthma (2005)
    Oxford, UK : Munksgaard International Publishers
    Allergy 60 (2005), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Mast cells constitute a significant proportion of cells infiltrating nasal polyp tissue, and epithelial cells may release stem cell factor (SCF), a cytokine with chemotactic and survival activity for mast cells. We aimed to assess the expression of SCF in human nasal polyp epithelial cells (NPECs) as related to patients’ clinical phenotypes. Nasal polyp tissues were obtained from 29 patients [including nine with aspirin (ASA)-hypersensitivity and 12 with bronchial asthma] undergoing polypectomy for nasal obstruction. Epithelial cells were obtained following 6-week culture of nasal polyps explants. The SCF released into the culture supernatant was assessed by enzyme-linked immunosorbent assay (ELISA) and total SCF mRNA in the polyp tissue was determined by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). For the whole group of patients, the number of polypectomies correlated with expression of SCF mRNA (r = 0.62; P 〈 0.005), SCF protein in the NPECs supernatants (r = 0.39; P 〈 0.05) and with density of mast cells in epithelial layer (r = 0.37; P 〈 0.05) and stromal layer (r = 0.5; P 〈 0.01) of nasal polyps. The SCF/β-actin mRNA ratios were significantly higher in ASA-hypersensitive (AH) asthmatics (median 0.97, range: 0.8–1.5) when compared with ASA-tolerant (AT) patients (median 0.5, range: 0.1–0.7; P 〈 0.001). The SCF protein concentration in NPEC supernatants was also significantly higher in AH asthmatics (median 1.10 pg/μg DNA, range: 0.4–1.9) when compared with AT patients (median 0.1 pg/μg DNA, range: 0.02–1.2; P 〈 0.001). In the subpopulation of ASA-sensitive asthmatics the number of poypectomies correlated also with the density of mast cells and eosinophils in the polyp tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.2005.00753.x
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  • 2
    Electronic Resource
    Electronic Resource
    Decreased apoptosis and distinct profile of infiltrating cells in the nasal polyps of patients with aspirin hypersensitivity (2002)
    Oxford, UK : Munksgaard International Publishers
    Allergy 57 (2002), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Patients with aspirin-hypersensitive rhinosinusitis/asthma suffer from a severe form of hyperplastic rhinosinusitis with recurrent polyposis. We aimed to assess the presence of apoptotic cells in nasal polyps from aspirin-hypersensitive (AH) and aspirin-tolerant (AT) patients with rhinosinusitis as related to the characteristics of local inflammation.Methods: Nasal polyps obtained from 16 AH patients and 36 AT patients (17 atopic and 19 nonatopic) were stained for eosinophils and metachromatic cells, and in parallel immunocytochemistry was performed to detect CD45RO+, HLA-DR+, CD8+ and CD68+ positive cells. Apoptotic cells were detected by a nick-end labelling technique, TUNEL.Results:  The density of apoptotic cells in AH polyps (5.5 + 1.5 cells/mm2) was significantly lower as compared to both atopic (18.7 + 3.8 cells/mm2; P 〈 0.02;) and nonatopic (21.3 + 5.2 cells/mm2; P 〈 0.01) AT polyps. The number of eosinophils, mast cells, and CD45RO+ cells were significantly increased in AH compared to AT polyps (P 〈 0.001), and the density of HLA-DR+ cells in AH patients was higher than in nonatopic (P 〈 0.02), but not in atopic AT patients. While in AH patients the duration of rhinosinusitis correlated inversely with the number of apoptotic cells (r = − 0.67; P 〈 0.04), in contrast, in AT atopic patients the duration of rhinosinusitis showed positive correlation with apoptosis (r = 0.89; P 〈 0.003).Conclusions:  We conclude, that decreased apoptosis of inflammatory cells in nasal polyps from ASA-hypersensitive patients, reflects a distinct mechanisms of local inflammation and may be related to persistence and severity of the disease in these patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2002.13508.x
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  • 3
    Electronic Resource
    Electronic Resource
    Glomerular Expression of α-Smooth Muscle Actin (α-SMA) in Idiopathic Mesangiocapillary Glomerulonephritis Type I. A Quantitative Study (1999)
    Springer
    International urology and nephrology 31 (1999), S. 827-838 
    Details
    ISSN: 1573-2584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Eighteen renal biopsy specimens from patients with mesangiocapillary glomerulonephritis type I (MCGN-I) evaluated by both light and electron microscopy as well as immunofluorescence microscopy and whose full clinical data were available were examined quantitatively. As control 10 biopsy specimens of kidneys removed because of trauma were used. Morphometric investigations were performed by means of a computer image analysis system to evaluate the glomerular expression of α-SMA in MCGN-I, as well as to determine whether this parameter could correlate with quantitatively analysed glomerular cells and mesangial areas. Another purpose of this study was to verify if the expression of α-SMA correlated with the intensity of glomerular leukocyte infiltrates in this glomerulopathy. The morphometric study revealed that mean values of the expression of α-SMA, total glomerular cells per total glomerular area, mesangium (% of total glomerular area), CD 45RB+, CD 43+, CD 20+ and CD 68+ cells were in MCGN-I patients increased in comparison with normal controls. Moreover, in the MCGN-I group, but not in controls, significant positive correlation existed between the glomerular expression of α-SMA and total glomerular cells per total glomerular area, mesangium (% of total glomerular area), as well as CD 45RB+ cells and CD 68+ cells. Significant positive correlation between α-SMA staining and total glomerular cells and mesangial areas suggested that increased α-SMA expression might be in MCGN-I an indicator of mesangial cell activation and mesangial matrix production. The significant positive correlation between the glomerular expression of α-SMA and glomerular CD 68+ cells requires further investigations to elucidate whether monocytes/macrophages play a role in the process of inducing myofibroblast phenotype in mesangial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007166117916
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