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  • 1
    Electronic Resource
    Electronic Resource
    Application of random amplified polymorphic DNA (RAPD) assays in identifying conserved regions of actinomycete genomes (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 128 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Various arbitrary primers as well as pUC18/19 ‘reverse’ sequencing primers were used for random amplified polymorphic DNA assays. Use of a modified reverse primer led to amplification of one major approx. 1100-bp band from the chromosomal DNA of all actinomycetes tested; however, the band was not found when DNAs from other bacteria were used in comparable experiments. Hybridization experiments showed that these bands all contained similar genomic regions. Subsequent sequencing of four of these fragments showed they each contained the sequence of the 3′ end of the 23S rRNA gene, the intergenic region and the start of the 5S rRNA gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07510.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Protein secretion in Streptomyces griseus N2-3-11: characterization of the secA gene and its growth phase-dependent expression (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 156 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The chromosomal region encoding the secA gene of Streptomyces griseus N2-3-11 was cloned and analyzed. The secA gene encodes a polypeptide of 939 aa with a molecular mass of 105 kDa. The growth defect of temperature sensitive Escherichia coli secA mutants was not restored by the S. griseus SecA. The secA promoter was analyzed and the transcriptional start point of the gene was determined. Northern blot and Western blot analyses revealed a growth phase dependent secA expression. The integration of an additional copy of the S. griseus secA gene into the genome of S. lividans TK23 had no visible effect on the efficiency of protein secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb12700.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning, nucleotide sequence and structural analysis of the Streptomyces galbus DSM40480 fda gene: the S. galbus fructose-1,6-bisphosphate aldolase is a member of the class II aldolases (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 197 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The fda gene of Streptomyces galbus DSM40480 encoding the fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) was cloned, sequenced and characterised. The fda gene encodes a protein of 341 amino acids with a molecular mass of 36.5 kDa and belongs to the class II aldolases. When the S. galbus fda gene was expressed in the Escherichia coli fda(ts) mutant NP315, the growth defect of the strain was complemented at temperatures 〉35°C. In Northern hybridisations, we identified an fda transcript of 1200 bp length. The transcript length indicates that the fda gene is transcribed from its own promoter. Attempts to isolate fda knock out mutants were not successful. Streptomyces lividans strains with a second copy of the fda gene were constructed and analysed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10582.x
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  • 4
    Electronic Resource
    Electronic Resource
    Positive and negative regulation of expression of the l-sorbose (sor) operon by SorC in Klebsiella pneumoniae (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 193-200 
    Details
    ISSN: 1617-4623
    Keywords: Sor operon ; Positive regulation ; Negative regulation ; Klebsiella pneumoniae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Klebsiella pneumoniae the gene products involved in the degradation of the ketose l-sorbose are encoded in the sor operon. It comprises, besides structural genes for uptake and catabolism, a promoter-proximal gene sorC, encoding a protein SorC of Mr 40 kDa, for which no enzymatic function has been detected. All sor genes are coordinately expressed and inducible by l-sorbose. Polar insertions and frameshift mutations in sorC cause a pleiotropic negative effect on the expression of all other sor genes. This defect is complemented in trans by the wild-type sorC + allele for frameshift mutations, but not for polar insertions. A single promoter for all sor genes, for which SorC is the activator, thus seems to be located in front of sorC. The repressor activity of SorC was demonstrated by complementation of constitutive sorC alleles with a sorC + allele leading to inducible expression of all sor genes, including sorC, which, as visualized by the use of a series of lacZ fusions, thus autoregulates its expression, both as an activator and a repressor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271552
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular analysis of the phosphoenolpyruvate-dependent l-sorbose: phosphotransferase system from Klebsiella pneumoniae and of its multidomain structure (1995)
    Springer
    Molecular genetics and genomics 246 (1995), S. 610-618 
    Details
    ISSN: 1617-4623
    Keywords: l-sorbose PTS ; sorFBAM ; Klebsiella pneumoniae ; New PTS family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have cloned a 3.4 kb DNA fragment from the chromosome of Klebsiella pneumoniae that codes for a phosphoenolpyruvate-dependent l-sorbose: phosphotransferase system (PTS). The cloned fragment was sequenced and four open reading frames coding for 135 (sorF), 164 (sorB), 266 (sorA) and 274 (sorM) amino acids, respectively, were found. The corresponding proteins could be detected in a T7 overexpression system, which yielded molecular masses of about 14000 for SorF, 19000 for SorB, 25000 for SorA and 27000 for SorM. SorF and SorB have all the characteristics of soluble and intracellular proteins in accordance with their functions as EIIASor and EIIBSor domains of the l-sorbose PTS. SorA and SorM, by contrast, are strongly hydrophobic, membrane-bound proteins with two to five putative transmembrane helices that alternate with a series of hydrophilic loops. They correspond to domains EIICSor and EIIDSor. The four proteins of the l-sorbose PTS resemble closely (27%–60%) the four subunits of a d-fructose PTS (EIIALev, EIIBLev, EIICLev, and EIIDLev) from Bacillus subtilis and the three subunits of the d-mannose PTS (EIIA,BMan, EIICMan, and EIIDMan) from Escherichia coli K-12. The three systems constitute a new PTS family, and sequence comparisons revealed highly conserved structures for the membranebound proteins. A consensus sequence for the membrane proteins was used to postulate a model for their integration into the membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298968
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