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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 145 (2001), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Chronic skin colonization with Staphylococcus aureus is a characteristic feature of atopic eczema (AE), and about 60% of S. aureus strains isolated from the skin of patients with AE secrete enterotoxins. Furthermore, IgE antibodies to S. aureus enterotoxins have been identified in 78% of patients with AE. Objectives To examine the S. aureus enterotoxin-induced histamine and leukotriene release of basophils from patients with AE. Methods Peripheral blood basophils from patients with AE were stimulated with the staphylococcal enterotoxins A, B, D, E and toxic shock syndrome toxin-1. Additionally, priming experiments were performed with interleukin (IL)-3, IL-8 and granulocyte/macrophage colony-stimulating factor followed by stimulation with S. aureus enterotoxins. Results In patients with AE, basophils secreted significantly higher amounts of histamine and leukotriene C4 (LTC4) than in healthy controls. The priming experiments showed additional histamine and LTC4 release in the group of AE patients. Conclusions Histamine and leukotriene generation from atopic basophils stimulated with staphylococcal enterotoxins may indicate a role for these toxins as possible allergens in at least a subgroup of patients with AE.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary In order to determine the genetic variability of roe deer (Capreolus capreolus) from 5 investigation areas in Rhineland-Palatinate, 7 isoenzyme systems from the liver tissues of 71 animals were analysed using gel electrophoresis. Of the 14 gene loci analysed, 9 were monomorph and 5 were polymorph. The average heterozygosity for all 5 populations was 4.92 %, the average number of alleles per gene locus was 1.5. There were, however, marked differences among the populations. One deer population isolated for 60 years showed a distinct loss in genetic variability compared to control populations. The causes of the investigated differences are discussed.
    Abstract: Résumé Afin d'apprécier, par voie biochimique, la variabilité génétique de chevreuils (Capreolus capreolus) de cinq territoires expérimentaux de Rhénanie, il a été parocédé à l'étude de 7 systèmes isoenzymatiques des tissus hépatiques par électrophorèse sur gel. Des 14 loci retenus, 9 étaient monomorphes, 5, polymorphes. Le taux moyen d'hétérozygotie des 5 populations s'élevait à 4,92 %, le nombre moyen d'allèles par locus, 1,5. Des différences marquantes ont cependant été relevées entre les différentes populations. Ainsi, une population, isolée depuis plus de 60 ans, révéla une diminution sensible de la variabilité génétique par rapport aux populations de contrôle. Les causes qui sont à l'origine de ce phénomène font l'objet d'une discussion.
    Notes: Zusammenfassung Um die biochemisch erfaßbare genetische Variabilität von Rehen (Capreolus capreolus) aus fünf Untersuchungsrevieren des Rheinlandes zu ermitteln, wurden bei insgesamt 71 Individuen sieben Isoenzymsysteme aus Lebergewebe mittels Gelelektrophorese untersucht. Von den erfaßten 14 Genloci waren neun mono- und fünf polymorph. Die durchschnittliche Heterozygotierate für alle fünf Populationen betrug 4,92 %. Die durchschnittliche Anzahl Allele pro Genlocus 1,5. Es fanden sich jedoch deutliche Unterschiede zwischen den einzelnen Populationen. So zeigte ein seit über 60 Jahren isolierter Rehbestand im Vergleich mit Kontrollpopulationen eine deutliche Abnahme der genetischen Variabilität. Die zugrundeliegenden Ursachen werden diskutiert.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4994
    Keywords: Green fluorescent protein (GFP) ; constitutive GFP expression ; visualization of GFP fluorescence ; UV exposure of mammalian cells ; Chinese Hamster Ovary (CHO) cell lines of different repair capabilities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Generation of Chinese Hamster Ovary (CHO) cell lines stably expressing green fluorescent protein (GFP) was achieved using a plasmid vector that encoded the red-shifted pCX-xGFP under the control of a strong hybrid promoter composed of a CMV enhancer and a β-actin/β-globin gene promoter. Cotransfection of the promoter-less pSV2-Neo helper plasmid transmitting neomycin resistance was followed by selection with the antibiotic G418. Constitutive GFP expression could be visualized in living and fixed cells using fluorescence spectroscopy, fluorescence microscopy, and flow cytometry. DNA repair-proficient (AA8) and deficient (UV5) CHO strains were used for survival tests after UVC irradiation. Cells carrying the GFP construct (AA8-pGFP, UV5-pGFP) show the same response to UV irradiation (colony forming ability) as their nontransformed parental cell lines (AA8, UV5). Using GFP as a marker for cell viability, cells were harvested after certain postirradiation growth periods and the numbers of GFP expressing cells and fluorescence intensities were determined by FACS analysis. Generally, GFP fluorescence in irradiated cells is not seen when cell membranes are damaged (leak-out of the soluble GFP). Irradiated cells without membrane damage express GFP continuously (leading to a dose-dependent increase in GFP contents).
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European journal of wildlife research 37 (1991), S. 69-77 
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary In order to determine the genetic variability of fallow deer in hunting districts in Northrhine-Westphalia, 6 isoenzyme systems from liver tissue of 56 sample animals were analyzed using gel electrophoresis. Of the 11 gene loci studied, 8 were monomorphic. The average heterozygotic rate of all populations was 1.4 %, the average number of alleles per gene loci was 1.37. No significant differences were found among the populations. Measures concerning the management of fallow deer for hunting are discussed.
    Abstract: Résumé Afin d'établir la variabilité génétique du Daim(Dama dama) sur le plan biochimique dans des territoires de Rhénanie-Westphalie, on a anlysé sur un ensemble de 56 individus six systèmes d'isoenzymes du tissu hépatique par électrophorèse. Des 11 loci géniques recensés, 8 étaient monomorphes. Le taux moyen d'hétérozygotie de toutes les populations s'élevait à 1,4 %, le nombre moyen d'allèles par locus génique à 1,37. Aucune différence significative ne fut décelée entre les populations. Une discussion porte sur les mesures de gestion cynégétique du Daim.
    Notes: Zusammenfassung Um die biochemisch erfaßbare genetische Variabilität von Damwild (Dama dama) in Revieren in Nordrhein-Westfalen zu ermitteln, wurden bei insgesamt 56 Individuen sechs Isoenzymsysteme aus Lebergewebe gelelektrophoretisch analysiert. Von den erfaßten 11 Genorten waren 8 monomorph. Die durchschnittliche Heterozygotierate aller Populationen betrug 1,4 %, die durchschnittliche Anzahl Allele pro Genort 1,37. Es fanden sich keine signifikanten Unterschiede zwischen den Populationen. Maßnahmen zur jagdlichen Bewirtschaftung des Damwildes werden diskutiert.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 9 (1963), S. 689-693 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 5 (1959), S. 406-406 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 5 (1959), S. 407-407 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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