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  • 1
    Digitale Medien
    Digitale Medien
    DNA profiling of bloodstains on linen pretreated with remedies used for cleaning and maintaining clothes (1991)
    Springer
    International journal of legal medicine 104 (1991), S. 273-275 
    Details
    ISSN: 1437-1596
    Schlagwort(e): DNA profiling ; Bloodstains ; Stain carrier ; DNA Profiling ; Blutspuren ; Spurenträger
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Leinenstoff wurde mit 20 Mitteln, die zur Wäschereinigung und -pflege verwendet werden, in normaler und sehr hoher Konzentration behandelt, absichtlich unzureichend oder nicht gewaschen, getrocknet und mit 20 μL Blut versetz. Hohe Rückstandskonzentrationen von zwei Waschmitteln und eines Aufhellers führten unregelmäßig zu partieller und totaler Inhibition der Restriktionsspaltung. Durch einen Dialyseschritt bei der Reinigung der DNA (Gill 1987) konnten die Störungen zuverlässig verhindert werden. Hochmolekulare DNA konnte in jedem Fall gewonnen werden, Bandshifts traten nicht auf.
    Notizen: Summary Linen was pretreated with 20 remedies for cleaning and maintaining clothes and 20 μl blood was applied on each sample and dried. Restriction enzyme digest of bloodstain DNA was irregularly inhibited by highly concentrated residues of 2 detergents and a stainremover and colour-brightener. An additional dialysis step to purify DNA (Gill 1987) reliably prevented disturbance. High molecular DNA was obtained in every case and bandshifts were not observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01369584
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  • 2
    Digitale Medien
    Digitale Medien
    Location and frequency of polymorphic positions in the mtDNA control region of individuals from Germany (1998)
    Springer
    International journal of legal medicine 111 (1998), S. 67-77 
    Details
    ISSN: 1437-1596
    Schlagwort(e): Key words DNA typing ; Mitochondrial DNA ; Non-coding region ; D-loop region ; Hypervariable ; regions ; PCR ; Sequencing ; Population study
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract In order to identify polymorphic positions and to determine their frequency in the human mitochondrial D-loop containing region, the mitochondrial DNA (mtDNA) control region of 200 unrelated individuals from Germany were amplified and directly sequenced. Sequence comparison led to the identification of 190 mitochondrial lineages as defined by 202 variable positions. The most frequently occurring lineage comprised 5 individuals, whereas 186 types of D-loop sequences were observed in only one individual. Of the sequences studied 7% are not unique but show at least one counterpart with an identical haplotype. The majority (61%) of the control regions investigated showed between four and eight nucleotide positions deviating from the reference sequence. The maximum number of deviations observed in a single control region was 18. The majority of the variable positions in the D-loop region (88%) are located within three hypervariable regions. Sequence variations are caused by nucleotide substitutions, insertions or deletions. As compared to insertions and deletions, nucleotide substitutions make up the vast majority of the mutations (90%). We have predominantly found transitions (75%) and a significantly lower frequency of transversions (15%) whereas insertions (6%) as well as deletions (4%) are rather rare. Upon sequencing the mitochondrial control region from 200 German Caucasians the genetic diversity was estimated at 0.99. The probability of two randomly selected individuals from a population having identical mtDNA types is 0.6%.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004140050117
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  • 3
    Digitale Medien
    Digitale Medien
    mtDNA as a tool for identification of human remains (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 205-209 
    Details
    ISSN: 1437-1596
    Schlagwort(e): DNA typing ; Mitochondrial DNA ; Non-coding region ; D-loop region ; PCR ; Sequencing ; Identification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract Two cases are presented in order to emphasize the importance of mitochondrial DNA in forensic medicine. The first case involved a charred body which could not be identified by morphological means because of severe destruction of all tissues. The parallel use of PCR methods using genomic DNA and sequencing of the mitochondrial d-loop region produced unequivocal and reproducible results. In the second case, various parts of a highly decomposed body were investigated. The application of standard PCR methods for genomic DNA proved unsuitable to answer the question whether the body parts belonged to the same body. However, when sequencing of mitochondrial DNA segments amplified from tissue and bone samples was performed, clearly interpretable results were obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01225519
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  • 4
    Digitale Medien
    Digitale Medien
    Allele frequencies for the VNTR locus D17S5 (YNZ22) in Hungary (1999)
    Springer
    International journal of legal medicine 112 (1999), S. 336-336 
    Details
    ISSN: 1437-1596
    Schlagwort(e): Key words Population genetics ; AMPFLP ; D17S5 ; YNZ22 ; Hungarian population
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract A population genetic study for the locus D17S5 was carried out on Caucasoids from the Budapest area consisting of 209 unrelated individuals. In this system we identified 13 different alleles and 51 genotypes. No new alleles were found and no significant deviation from Hardy-Weinberg equilibrium was observed. The power of discrimination was estimated at 0.958, the power of exclusion at 0.60 and the observed heterozygosity at 0.80.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004140050264
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  • 5
    Digitale Medien
    Digitale Medien
    PCR typing of human semen stains after SEM-EDX examination (1999)
    Springer
    International journal of legal medicine 112 (1999), S. 376-379 
    Details
    ISSN: 1437-1596
    Schlagwort(e): Key words Semen ; Scanning electron microscopy ; Electron dispersive x-ray microanalysis ; DNA typing ; PCR
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract There is an increasing demand to use scanning electron microscopy in the forensic analysis of biological samples. Such analyses are routinely used for the investigation of blood stains, seminal stains, diatoms, residues on wounds and residues and trace elements in gunshot powder. The same samples are sometimes also required for identification via DNA analysis, e.g. blood stains, seminal stains or epidermal cells. The ionising radiation provokes damage to DNA and also to membrane and proteine structure. The question therefore arises whether the usual sequence of such an investigation, i.e. prior application of SEM and afterwards analysis of DNA, can affect the success rate of the DNA analysis. We have therefore experimentally exposed semen samples to a defined electron beam for different time intervals varying between 1 and 25 min and afterwards performed quantitative and qualitative DNA analysis. Our studies revealed that sample treatment with an electron beam does not interfere with subsequent DNA typing by various currently used forensic PCR systems.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004140050017
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  • 6
    Digitale Medien
    Digitale Medien
    Identifikation eines skelettierten Kleinkind-Schädels durch die molekularbiologische Untersuchung von Hirnresten (1998)
    Springer
    Rechtsmedizin 8 (1998), S. 109-111 
    Details
    ISSN: 1434-5196
    Schlagwort(e): Key words Identification ; Forensic osteology ; DNA typing ; PCR ; Brain ; Bone ; Schlüsselwörter Identifikation ; Forensische ; Osteologie ; DNA-Typisierung ; PCR ; Gehirn ; Knochen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Ein weitgehend skelettierter Kleinkind-Schädel konnte mittels forensisch-anthropologischer Befunderhebung klassifiziert und mittels erfolgreicher DNA-PCR-Typisierung am Hirnbrei, nicht jedoch am Schädelknochen identifiziert werden. Die vorgestellte Kasuistik belegt eindrücklich, daß eine sorgfältige forensisch-anthropologische Befunderhebung eine wesentliche Grundlage für die molekularbiologische Identifizierung an Skeletteilen bildet und daß auch an fortgeschritten fäulnisverändertem Gehirn bei feuchtem Lagerungsmilieu eine DNA-PCR-Typisierung erfolgreich sein kann.
    Notizen: Abstract The skull of a small child, which was largely devoid of soft tissue, was classified according to forensic/anthropological findings and could be identified on the basis of DNA typing by PCR using residual brain tissue, but not bone. This case illustrates the fact that careful forensic/anthropological investigation is an important basis for molecular biological identification from skeletal remains and that DNA typing by PCR may even be possible on brain tissue that has been exposed to damp conditions and is an advanced state of decomposition.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001940050041
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