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  • 1
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 176 (1973), S. 121-123 
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Total protein content of preimplantation mouse embryos was measured at 06.00 and 16.00 hours on day 5 of pregnancy (day 1, the day of vaginal plug formation). During this ten hour period, just prior to implantation, the amount of protein increased from 23 to 35 ng per blastocyst; a net increase of 52%. In contrast, protein content was unchanged in delayed implanting embryos recovered from ovariectomized animals during a comparable period on days 5 or 10 (day 5, 24 ng at 06.00 hours and 24 ng at 16.00 hours; day 10, 29 ng at 06.00 hours and 30 ng at 16.00 hours).These results demonstrate that the different uptake and incorporation of amino acids between normal and delayed implanting embryos (Weitlauf and Greenwald, '65, '68; Weitlauf, '73) reflect a difference in the actual net synthesis of protein by the two types of blastocysts.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 159 (1967), S. 249-253 
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Five-day-old mouse blastocysts were transferred into the oviducts of recipients on the second day of pregnancy. S35 methionine was then injected into the recipients and the blastocysts and native 2-celled eggs were recovered six hours later. Radioautographs reveal that the blastocysts incorporate S35 methionine while exposed to the tubal environment to the same degree that they would in the uterus. However, the 2-celled eggs in the same oviducal environment incorporate little or no methionine. It is therefore concluded that the difference in the incorporation of S35 methionine is due to maturational changes in the blastocyst rather than to a deficiency of the labelled amino acid in the tubal lumen.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 132 (1971), S. 103-107 
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The level of protein synthesis by hamster embryos in vivo, as inferred from the incorporation of 35S methionine, was determined for the four days of the preimplantation period by radioautography. The embryos incorporated 35S methionine throughout the preimplantation period. Furthermore, the hamster eggs continued to incorporate 35S methionine following ovariectomy. Therefore, the pattern of protein synthesis in hamster eggs differs from the mouse with its low levels in cleaving eggs and in blastocysts following ovariectomy and high levels in blastocysts influenced by estrogen and progesterone.This finding is of interest because unimplanted mouse embryos remain viable in ovariectomized females for many days, whereas hamster embryos rapidly degenerate. It is suggested that the inability of hamster eggs to reduce the level of protein synthesis and become “dormant” may prevent their survival if implantation is blocked by ovariectomy.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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