ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Time-switched detectors
;
UV detection
;
Fluorimetric detection
;
Vitamin A
;
Vitamin E
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An HPLC method utilizing a UV and a fluorimetric detector linked in series is described. By use of a simple integrator-controlled time-switched relay, analysis of serum vitamin A and E is accomplished on the same chromatogram and at optimum sensitivity for each detector. A single internal standard (retinyl acetate) monitored only by the UV detector permits measurement of both vitamins over a wide linear range. Precision of the assays is satisfactory, both on a within-day and on a day-to-day basis. Recoveries of both vitamins are virtually 100% whilst sensitivity is 2 μg/L (retinol) and 0.05 mg/L (α-tocopherol).
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240090506
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