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  • 1
    Electronic Resource
    Electronic Resource
    A HARP SEAL × HOODED SEAL HYBRID (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 13 (1997), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this study we report the first documentation of mating between harp (Phoca groenlandica) and hooded seals (Cystophora cristata). The production of this hybrid was quite unusual, being the result of a cross between parents of different genera which are morphologically dissimilar and have quite different mating behavior and dramatically different body sizes. The molecular techniques (mtDNA and macrosatellite nuclear DNA banding patterns) used in this study will undoubtedly be applied widely to many different taxa in the near future, allowing us to re-examine many suspected cases of hybridization among marine mammals and, in a larger context, the meaning of the species concept.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.1997.tb00652.x
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  • 2
    Electronic Resource
    Electronic Resource
    SEXUAL COMPOSITION and ANALYSIS OF REPRODUCTIVE FEMALES IN THE NORTH ATLANTIC RIGHT WHALE, EUBALAENA GLACIALIS, POPULATION (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 10 (1994), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To test hypotheses involving reproduction and demographics, the sex of individuals must be established, but many species of Cetacea are not obviously dimorphic. In the North Atlantic right whale, Eubalaena glacialis, population, the sex of 61 males and 55 females had been determined previously by observation of the urogenital region, and the sex of 43 more females had been inferred from repeated sightings with a calf. To confirm the sex of some of these animals and to identify the sex of mote animals, genomic DNA was isolated from skin samples of 95 individual right whales (54 from among those described above and 41 additional recognizable individuals). The DNA was surveyed using the human Y-chromosome probe pDP1007. With EcoRI-digested DNA, a clear, sex-discriminating banding pattern was apparent. This method verified the sex of all 54 animals whose sex was previously known or inferred and identified the sex of an additional 41 recognizable individuals. A total of 89 male and 111 female right whales was identified in the population. The most unbiased estimate of sex ratio available is the 36 male and 34 female calves identified by genital morphology and DNA techniques. The sex ratio of this sample does not differ significantly from unity (P = 0.811). Only 38% (58/152) of the females in the North Atlantic population are known to have been reproductively successful compared with 54% in the population of right whales in the western South Atlantic. The population growth rate reported for the North Atlantic population is only 33% of that reported for right whales in the South Atlantic. Thirteen adult North Atlantic females have been identified that have not been known to calve during the past 11 yr. These data suggest that the absence of measurable recovery may be due to a combination of fewer actively reproducing females and lower reproductive rates of some females.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.1994.tb00481.x
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  • 3
    Electronic Resource
    Electronic Resource
    DNA marker analysis detects multiple maternity and paternity in single broods of the lesser snow goose (1987)
    [s.l.] : Nature Publishing Group
    Nature 326 (1987), S. 392-394 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1 Genotypes of nest attendants and goslings of four lesser snow goose families DNA marker DQSG1-AI DQSG1-AII DQSG1-CI DQSG5-BI DQSG5-BH DQSG5-BIU DQSG6-AIS DQSG7-BI DQSG7-BII DQSG7-BIII DQSG8-BI DQSG8-BII DQSG8-DI ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/326392a0
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  • 4
    Electronic Resource
    Electronic Resource
    Translational control in the mealworm, Tenebrio molitor (1975)
    [s.l.] : Nature Publishing Group
    Nature 256 (1975), S. 734-735 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We decided to look at changes of tRNAs during the development of Tenebrio and compare them with changes observed during the development of Drosophila*'10. Transfer RNA and aminoacyl-tRNA synthetases were extracted by the methods described previously8. Aminoacylation conditions were established ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/256734a0
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  • 5
    Electronic Resource
    Electronic Resource
    An evolutionary model for the insect vitellins (1982)
    Springer
    Journal of molecular evolution 18 (1982), S. 405-413 
    Details
    ISSN: 1432-1432
    Keywords: Insect Vitellins ; Immunological crossreactivity ; Evolutionary model ; Amino acid composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Insects can be divided into three groups based on the sizes of the polypeptide constituents of their vitellogenins and vitellins. In order to determine the relationships between these groups, antisera to the vitellins of seven insects from six taxonomic orders were used to assess immunological cross-reactivity. Antigenic relatedness was observed only between vitellins from species within the same family. Amino acid compositional data for vitellins from nine species were used to assess homology by difference matrices. The SΔQ values were similar for both intra-order and inter-order comparisons and strongly suggested relatedness. The SΔn comparisons supported the immunological data that indicated that the vitellins were evolving rapidly. For most insect vitellins there are two distinct size classes of polypeptides that seem to be derived from a single asymmetric proteolytic cleavage of a precursor. We propose a model that suggests that the different size polypeptides represent distinct domains and that in the evolution of the vitellogenin genes of the Diptera and Hymenoptera there has been domain elimination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01840888
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  • 6
    Electronic Resource
    Electronic Resource
    Stimulation of extracellular protein production in Bacillus brevis 47 (1989)
    Springer
    Applied microbiology and biotechnology 31 (1989), S. 338-341 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Bacillus brevis 47 was cultivated in 2-1 fermentors to study the effect of medium supplementation on extracellular protein production. Additional polypeptone, when supplied initially or at 12 h (late exponential phase), had little stimulatory effect on extracellular protein levels, which reached 6–7 g/l after 48h. A large increase in protein production was observed, however, when polypeptone was added at 21 h (stationary phase). This addition resulted in the accumulation in the medium of 14 g/l protein after 48 h, and a total of 16 g/l when cell-bound protein was included. In all cases, glucose was consumed only very slowly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257600
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  • 7
    Electronic Resource
    Electronic Resource
    Continuous alpha-amylase production using Bacillus amyloliquefaciens adsorbed on an ion exchange resin (1988)
    Springer
    Applied microbiology and biotechnology 28 (1988), S. 8-13 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A method for the continuous production of extracellular alpha amylase by surface immobilized cells of Bacillus amyloliquefaciens NRC 2147 has been developed. A large-pore, macroreticular anionic exchange resin was capable of initially immobilizing an effective cell concentration of 17.5 g DW/1 (based on a total reactor volume of 160 ml). The reactor was operated continuously with a nutrient medium containing 15 g/l soluble starch, as well as yeast extract and salts. Aeration was achieved by sparging oxygen enriched air into the column inlet. Fermentor plugging by cells was avoided by periodically substituting the nutrient medium with medium lacking in both soluble starch and yeast extract. This fermentor was operated for over 200 h and obtained a steady state enzyme concentration of 18700 amylase activity units per litre (18.7 kU/l), and an enzyme volumetric productivity of 9700 amylase activity units per litre per hour (9.7 kU/l-h). Parallel fermentations were performed using a 2 l stirred vessel fermentor capable of operation in batch and continuous mode. All fermentation conditions employed were identical to those of the immobilized cell experiments in order to assess the performance of the immobilized cell reactor. Batch stirred tank operation yielded a maximum amylase activity of 150 kU/l and a volumetric productivity of 2.45 kU/l-h. The maximum cell concentration obtained was 5.85 g DW/l. Continuous stirred tank fermentation obtained a maximum effluent amylase activity of 6.9 kU/l and a maximum enzyme volumetric productivity of 2.73 kU/l-h. Both of these maximum values were observed at a dilution rate of 0.345 l/h. The immobilized cell reactor was observed to achieve larger volumetric productivities than either mode of stirred tank fermentation, but achieved an enzyme activity concentration lower than that of the batch stirred tank fermentor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00250489
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of an α-satellite repeated sequence from human chromosome 22 (1986)
    Springer
    Chromosoma 94 (1986), S. 228-234 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We constructed a library in λIL47.1 with DNA isolated from flow-sorted human chromosome 22. Over 50% of the recombinants contained the same highly repetitive sequence. When this sequence was used to probe Southern blots of EcoRI-digested genomic DNA, a ladder of bands with increments of about 170 bp was observed. This sequence comigrates with satellite III in Ag+/Cs2SO4 gradients and may account for at least part of the 170 bp Hae III ladder seen in isolated satellite III DNA. Partial sequence analysis revealed homology to the 171 bp monomeric repeat unit of α-R1-DNA and the X specific α-satellite consensus sequence. After low stringency in situ hybridization, silver grains were found over the centromeres of a number of chromosomes. Under high stringency conditions, however, the labeling was concentrated over the centromeric region of chromosome 22. This localization was confirmed using DNA from a panel of human/hamster cell lines which showed that the homologous 2.1 and 2.8 kb EcoR1 restriction fragments were chromosome 22 specific. These clones therefore contain chromosome 22 derived α-satellite sequences analogous to other chromosome-specific satellite sequences described previously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288497
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  • 9
    Electronic Resource
    Electronic Resource
    Linkage analysis of seven kindreds with the X-linked lymphoproliferative syndrome (XLP) confirms that the XLP locus is near DXS42 and DXS37 (1989)
    Springer
    Human genetics 〈Berlin〉 82 (1989), S. 354-358 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Analysis of seven kindreds indicates that the XLP locus exhibits 1% recombination with DXS42 (lod = 17.5) and no recombination with DXS37 (lod = 13.3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273997
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of a lymphoblastoid line deleted for lambda immunoglobulin genes (1995)
    Springer
    Immunogenetics 41 (1995), S. 359-365 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract While characterizing the cat eye syndrome (CES) supernumerary chromosome for the presence of λ immunoglobulin gene region sequences, a lymphoblastoid cell line from one CES patient was identified in which there was selection of cells deleted for some IGLC and IGLV genes. Two distinct deletions, one on each chromosome 22, were identified, presumably arising from independent somatic recombination events occuring during B-lymphocyte differentiation. The extent of the deleted region was determined using probes from the various IGLV subgroups and they each cover at least 82 kilobases. The precise definition of the deletions was not possible because of conservation of some restriction sites in the IGLV region. The cell line was used to map putative IGLV genes within the recombinant phage λVλ135 to the distal part of the IGLV gene region. Since the deletions are relatively small, the cell line will be valuable for mapping IGLV genes in the distal part of this region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00163993
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